Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	884,506	G→A	L278L (CTG→CTA)	mdfA →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	884,506	0	G	A	100.0%	192.0 / NA	54	L278L (CTG→CTA)	mdfA	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/27);  total (27/27)

TGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTAT  >  NC_000913/884376‑884641                                                                                                                                   |                                                                                                                                        tGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTcgcg                                                                                                                                 <  2:969533/139‑1 (MQ=255)  gCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCgcgc                                                                                                                                >  1:272313/1‑139 (MQ=255)  gCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCgcgc                                                                                                                                >  2:481272/1‑139 (MQ=255)    tGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGcgccg                                                                                                                              <  2:909509/139‑1 (MQ=255)     ggCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGcgccgc                                                                                                                             >  1:807001/1‑139 (MQ=255)       cGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCAc                                                                                                                           <  2:272313/139‑1 (MQ=255)            aTCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTAc                                                                                                                      <  1:948147/139‑1 (MQ=255)                cccAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTc                                                                                                                  <  1:1101905/139‑1 (MQ=255)                           aTTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGAttatta                                                                                                       >  2:713864/1‑139 (MQ=255)                                 atcatTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAAcc                                                                                                                                      <  1:117160/102‑1 (MQ=255)                                 atcatTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAAcc                                                                                                                                      >  2:117160/1‑102 (MQ=255)                                   catTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGggcggc                                                                                               <  1:1004369/139‑1 (MQ=255)                                      tACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTgg                                                                                            >  2:504138/1‑139 (MQ=255)                                      tACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTgg                                                                                            >  1:650372/1‑139 (MQ=255)                                      tACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTgg                                                                                            <  2:1012633/139‑1 (MQ=255)                                         cGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCg                                                                                         <  1:481272/139‑1 (MQ=255)                                         cGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCg                                                                                         >  2:166826/1‑139 (MQ=255)                                         cGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCg                                                                                         >  1:1099104/1‑139 (MQ=255)                                              aGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTAt                                                                                    >  1:136895/1‑139 (MQ=255)                                                cAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGa                                                                                  <  1:478120/139‑1 (MQ=255)                                                cAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGa                                                                                  >  2:736804/1‑139 (MQ=255)                                                           cTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGatt                                                                                                           <  1:385449/103‑1 (MQ=255)                                                           cTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGatt                                                                                                           >  2:385449/1‑103 (MQ=255)                                                             aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGt                                                                     >  1:902154/1‑139 (MQ=255)                                                                     ggCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTc                                                                                                                  <  1:403405/86‑1 (MQ=255)                                                                     ggCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTc                                                                                                                  >  2:403405/1‑86 (MQ=255)                                                                       cTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCg                                                           >  1:849463/1‑139 (MQ=255)                                                                         tgctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGc                                                         <  1:166826/139‑1 (MQ=255)                                                                         tgctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGc                                                         >  1:191489/1‑139 (MQ=255)                                                                                        ttttCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAAcc                                                                                                                                      <  2:819668/47‑1 (MQ=255)                                                                                        ttttCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAAcc                                                                                                                                      >  1:819668/1‑47 (MQ=255)                                                                                           tCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCa                                       <  2:749363/139‑1 (MQ=255)                                                                                              gggCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCAcgc                                    <  1:504138/139‑1 (MQ=255)                                                                                              gggCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCAcgc                                    <  2:454165/139‑1 (MQ=255)                                                                                               ggCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCAcgcg                                   <  1:713864/139‑1 (MQ=255)                                                                                                   ttAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCg                                                                                                                 >  2:122480/1‑57 (MQ=255)                                                                                                   ttAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCg                                                                                                                 <  1:122480/57‑1 (MQ=255)                                                                                                   ttAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTAtt                               >  1:516037/1‑139 (MQ=255)                                                                                                             gTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGAc                     >  2:264951/1‑139 (MQ=255)                                                                                                             gTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGAc                     >  1:585299/1‑139 (MQ=255)                                                                                                             gTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGAc                     >  1:711681/1‑139 (MQ=255)                                                                                                               aaCTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTg                   <  1:264951/139‑1 (MQ=255)                                                                                                                aCTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGc                  <  2:807001/139‑1 (MQ=255)                                                                                                                aCTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGc                  >  1:420046/1‑139 (MQ=255)                                                                                                                 cTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGcc                 <  2:585299/139‑1 (MQ=255)                                                                                                                      tGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGtt            <  1:306821/139‑1 (MQ=255)                                                                                                                      tGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGtt            <  1:732620/139‑1 (MQ=255)                                                                                                                          aGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTa           <  2:45160/136‑1 (MQ=255)                                                                                                                          aGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTa           >  1:45160/1‑136 (MQ=255)                                                                                                                          aGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGt        >  2:1005845/1‑139 (MQ=255)                                                                                                                            cgcgTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTa           <  2:1044365/134‑1 (MQ=255)                                                                                                                            cgcgTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTa           >  1:1044365/1‑134 (MQ=255)                                                                                                                                tCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTAt  <  2:725303/139‑1 (MQ=255)                                                                                                                                tCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTAt  >  2:775351/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                        TGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTAT  >  NC_000913/884376‑884641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑