Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,219,119	A→G	Y175C (TAC→TGC)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,219,119	0	A	G	100.0%	174.6 / NA	46	Y175C (TAC→TGC)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/23);  total (23/23)

GCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCC  >  NC_000913/4218984‑4219246                                                                                                                                        |                                                                                                                                gccgcTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCcattgcat                                                                                                                              <  2:904044/139‑1 (MQ=255)   cgcTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCcattgcatta                                                                                                                            >  1:460112/1‑139 (MQ=255)               aTTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGa                                                                                                                >  1:1008105/1‑139 (MQ=255)               aTTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGa                                                                                                                >  1:717431/1‑139 (MQ=255)               aTTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGa                                                                                                                >  2:63747/1‑139 (MQ=255)                   cccgcccgCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGa                                                                                                            <  1:125732/139‑1 (MQ=255)                        ccgcTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGc                                                                                                       <  2:460112/139‑1 (MQ=255)                           cTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTgg                                                                                                    >  2:955797/1‑139 (MQ=255)                           cTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTgg                                                                                                    <  2:273630/139‑1 (MQ=255)                                  aaGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGgacaga                                                                                             <  2:717431/139‑1 (MQ=255)                                  aaGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGgacaga                                                                                             <  1:996775/139‑1 (MQ=255)                                    gACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGgacagaca                                                                                           <  1:125587/139‑1 (MQ=255)                                        ttCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGTCAAc                                                                                         >  2:574570/1‑137 (MQ=255)                                        ttCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGTCAAc                                                                                         <  1:574570/137‑1 (MQ=255)                                        ttCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCt                                                                                       >  1:170576/1‑139 (MQ=255)                                              ccGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGa                                                                                 <  1:504590/139‑1 (MQ=255)                                                 aTCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGaa                                                                                <  2:656929/137‑1 (MQ=255)                                                 aTCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGaa                                                                                >  1:656929/1‑137 (MQ=255)                                                                ctctACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGc                                                               >  1:869191/1‑139 (MQ=255)                                                                  ctACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGcga                                                             >  2:581262/1‑139 (MQ=255)                                                                  ctACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGcga                                                             >  2:1034992/1‑139 (MQ=255)                                                                      tGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGcgaacga                                                         <  1:955797/139‑1 (MQ=255)                                                                       gATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGcgaacgaa                                                        >  2:514003/1‑139 (MQ=255)                                                                        aTGCGCGTTATCAGCGACCTACCGCTGTGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCgaacgaac                                                       >  2:388744/1‑139 (MQ=255)                                                                           cgcgTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGt                                                    >  1:90583/1‑139 (MQ=255)                                                                             cgTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGttt                                                  >  1:560097/1‑139 (MQ=255)                                                                                  tCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCg                                             <  2:414258/139‑1 (MQ=255)                                                                                        aCCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATaa                                       >  1:35258/1‑139 (MQ=255)                                                                                            aCCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAg                                                                              >  1:416327/1‑96 (MQ=255)                                                                                            aCCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAg                                                                              <  2:416327/96‑1 (MQ=255)                                                                                                   cgcctgcgcTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCt                                                                                                      <  1:967285/65‑1 (MQ=255)                                                                                                   cgcctgcgcTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCt                                                                                                      >  2:967285/1‑65 (MQ=255)                                                                                                    gcctgcgcTGGCAGAATAAAAGCCGTGACATCCATTGCA‑TATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCctggctg                          >  2:196779/1‑139 (MQ=255)                                                                                                           cTGGCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGc                    >  1:972713/1‑139 (MQ=255)                                                                                                             ggCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCaa                  <  2:170576/139‑1 (MQ=255)                                                                                                              gCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCaaa                 <  2:90583/139‑1 (MQ=255)                                                                                                              gCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCaaa                 <  1:134183/139‑1 (MQ=255)                                                                                                              gCAGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCaaa                 <  1:174934/139‑1 (MQ=255)                                                                                                                aGAATAAAAGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACt               <  1:1034992/139‑1 (MQ=255)                                                                                                                     aaaaGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATca          <  2:560097/139‑1 (MQ=255)                                                                                                                     aaaaGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATca          <  2:453420/139‑1 (MQ=255)                                                                                                                       aaGCCGTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATcaca        <  2:972713/139‑1 (MQ=255)                                                                                                                            gTGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTc   >  1:99263/1‑139 (MQ=255)                                                                                                                             tGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTcc  >  2:39491/1‑139 (MQ=255)                                                                                                                             tGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTcc  <  1:527774/139‑1 (MQ=255)                                                                                                                             tGACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTcc  >  2:9783/1‑139 (MQ=255)                                                                                                                              gACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGa                                                                                 >  2:351173/1‑59 (MQ=255)                                                                                                                              gACATCCATTGCATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGa                                                                                 <  1:351173/59‑1 (MQ=255)                                                                                                                                        |                                                                                                                                GCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCC  >  NC_000913/4218984‑4219246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑