Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,607,066	A→G	V212A (GTT→GCT)	rsmC ←	16S rRNA m(2)G1207 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,607,066	0	A	G	100.0%	73.9 / NA	21	V212A (GTT→GCT)	rsmC	16S rRNA m(2)G1207 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/12);  total (9/12)

CGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTACCGACATCCAGACCGTCGCGGC  >  NC_000913/4606932‑4607171                                                                                                                                       |                                                                                                          cGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGa                                                                                                       >  1:862744/1‑139 (MQ=255)         aCGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGc                                                                                               >  1:345045/1‑139 (MQ=255)          cGTTGCTGGCTAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGcc                                                                                              <  1:295676/139‑1 (MQ=255)          cGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGcc                                                                                              <  1:372719/139‑1 (MQ=255)           gTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGCGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGccc                                                                                             >  1:146086/1‑139 (MQ=255)            ttGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCcg                                                                                            >  2:1007871/1‑139 (MQ=255)               cTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCgcgc                                                                                         <  2:345045/139‑1 (MQ=255)                  gCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTGCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCcac                                                                                      <  2:482432/139‑1 (MQ=255)                     aaGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCTCGCCACAGc                                                                                   >  1:656528/1‑139 (MQ=255)                                 tCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCACCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCa                                                                       >  1:441783/1‑139 (MQ=255)                                  cAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCAc                                                                      <  2:862943/139‑1 (MQ=255)                                                 cAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTTCCTTTcctttg                                                       <  1:644926/139‑6 (MQ=255)                                                 cAAGTGTTGCGCGGC‑GGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGc                                                      <  2:292211/139‑1 (MQ=255)                                                              gctggctTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAg                                                  <  1:764548/131‑1 (MQ=255)                                                              gctggctTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAg                                                  >  2:764548/1‑131 (MQ=255)                                                               ctggctTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCg                                         >  1:105692/1‑139 (MQ=255)                                                                    ttCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGagc                                    <  1:1007871/139‑1 (MQ=255)                                                                                ggCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTa                        <  2:105692/139‑1 (MQ=255)                                                                                  cAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTAcc                      <  2:441783/139‑1 (MQ=255)                                                                                  cAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTAcc                      <  2:59216/139‑1 (MQ=255)                                                                                                      agaCGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTACCGACATCCAGACCGTCGCGGc  >  2:549333/1‑139 (MQ=255)                                                                                                                                       |                                                                                                          CGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTACCGACATCCAGACCGTCGCGGC  >  NC_000913/4606932‑4607171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑