Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,116,482 T→C Y34C (TAC→TGC)  lpxL ← lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,116,4820TC95.0% 132.7 / ‑1.7 40Y34C (TAC→TGC) lpxLlauryl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (19/19);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

CATTTTACGGCGTTCTTGTTCGCTCATTTCCGGGAAGCACAGTTCCAGGTTGCGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCA  >  NC_000913/1116349‑1116612
                                                                                                                                     |                                                                                                                                  
caTTTTACGGCGTTCTTGTTCGCTCATTTCCGGGAAGCACAGTTCCAGGTTGCGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGc                                                                                                                               >  2:422030/1‑139 (MQ=255)
     tACGGCGTTCTTGTTCGCTCATTTCCGGGAAGCACAGTTCCAGGTTGCGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTg                                                                                                                          <  1:623353/139‑1 (MQ=255)
     tACGGCGTTCTTGTTCGCTCATTTCCGGGAAGCACAGTTCCAGGTTGCGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTg                                                                                                                          <  1:609399/139‑1 (MQ=255)
           gTTCTTGTTCGCTCATTTCCGGGAAGCACAGTTCCAGGTTGCGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGAc                                                                                                                    >  2:650873/1‑139 (MQ=255)
                         aTTTCCGGGAAGCACAGTTCCAGGTTGCGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGc                                                                                                      <  1:422030/139‑1 (MQ=255)
                                        aGTTCCAGGTTGCGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAgg                                                                                       >  1:109923/1‑139 (MQ=255)
                                        aGTTCCAGGTTGCGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAgg                                                                                       <  1:282235/139‑1 (MQ=255)
                                               ggTTGCGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCa                                                                                >  2:442947/1‑139 (MQ=255)
                                                    cGATGCACTATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGATAACCAATAAc                                                                           >  2:721093/1‑139 (MQ=255)
                                                    cGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAAcc                                                                                          >  1:240877/1‑124 (MQ=255)
                                                    cGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAAcc                                                                                          <  2:240877/124‑1 (MQ=255)
                                                    cGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAAc                                                                           >  2:1108303/1‑139 (MQ=255)
                                                    cGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCCAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAAc                                                                           >  2:164981/1‑139 (MQ=255)
                                                    cGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAATTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAAc                                                                           <  1:721093/139‑1 (MQ=255)
                                                      aTGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGa                                                                                                                     <  1:1032460/95‑1 (MQ=255)
                                                      aTGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGa                                                                                                                     >  2:1032460/1‑95 (MQ=255)
                                                         cacaATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATa                                                                                                 <  2:835479/112‑1 (MQ=255)
                                                         cacaATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATa                                                                                                 >  1:835479/1‑112 (MQ=255)
                                                         cacaATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGa                                                                      >  2:555956/1‑139 (MQ=255)
                                                              tttttGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAg                                                                 <  1:900142/139‑1 (MQ=255)
                                                                tttGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCa                                                               <  2:1076579/139‑1 (MQ=255)
                                                                   gcgcGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATg                                                                    <  1:776524/131‑1 (MQ=255)
                                                                   gcgcGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATg                                                                    >  2:776524/1‑131 (MQ=255)
                                                                      cgtcgtTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCgg                                                         >  2:449599/1‑139 (MQ=255)
                                                                        tcgtTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTg                                                       >  1:166338/1‑139 (MQ=255)
                                                                              cATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAAc                                                 <  2:109923/139‑1 (MQ=255)
                                                                                   aaCGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTggg                                            >  2:1069012/1‑139 (MQ=255)
                                                                                    aCGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGt                                           <  1:650873/139‑1 (MQ=255)
                                                                                       tAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGa                                        <  1:747943/139‑1 (MQ=255)
                                                                                        aaCGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAAATGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGAt                                       <  1:164981/139‑1 (MQ=255)
                                                                                             cAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTc                                  >  1:483251/1‑139 (MQ=255)
                                                                                                             caACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGtttt                  >  2:154800/1‑139 (MQ=255)
                                                                                                             caACCGAGGCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGtttt                  >  1:91807/1‑139 (MQ=255)
                                                                                                                    ggCGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCt           >  1:30418/1‑139 (MQ=255)
                                                                                                                      cGGTAGATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAAACCTGTTTTTCAACCTAt         <  1:449599/139‑1 (MQ=255)
                                                                                                                           gATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCggg    <  2:30418/139‑1 (MQ=255)
                                                                                                                           gATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCggg    <  2:166338/139‑1 (MQ=255)
                                                                                                                           gATAACCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCggg    <  1:555956/139‑1 (MQ=255)
                                                                                                                               aCCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCa  >  2:407834/1‑137 (MQ=255)
                                                                                                                               aCCGGGCAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCa  <  1:407834/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                  
CATTTTACGGCGTTCTTGTTCGCTCATTTCCGGGAAGCACAGTTCCAGGTTGCGATGCACAATTTTTGCGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCA  >  NC_000913/1116349‑1116612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑