| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,806,042 | (C)6→7 | coding (42/759 nt) | ydiY ← | acid‑inducible putative outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,806,036 | 1 | . | C | 94.2% | 174.3 / 2.5 | 52 | coding (48/759 nt) | ydiY | acid‑inducible putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base C (25/24); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑C‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGG > NC_000913/1805905‑1806169 | cTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑c‑c‑ccccc < 1:1082359/139‑2 (MQ=255)cTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑c‑c‑ccccc < 1:135941/139‑2 (MQ=255)cTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑c‑c‑ccccc > 2:1082359/1‑138 (MQ=255) ttttCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAGACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑c‑c‑ccccc < 1:186847/138‑2 (MQ=255) ttttCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑c‑c‑cccccg < 1:564048/139‑3 (MQ=255) ttttCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑c‑c‑ccccc > 2:186847/1‑137 (MQ=255) tttCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑c‑c‑cccccgc < 1:668142/139‑4 (MQ=255) cTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑C‑C‑CCCCGCCAACATAACGAt < 2:1216998/139‑1 (MQ=255) aTTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATgg > 2:639813/1‑139 (MQ=255) aTTGTGCAAGATATCCTGCATTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATgg > 2:17065/1‑139 (MQ=255) tgCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAgg > 1:1274486/1‑139 (MQ=255) tgCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAgg > 1:552773/1‑139 (MQ=255) tgCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAgg > 1:493315/1‑139 (MQ=255) cAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa < 1:259885/139‑1 (MQ=255) cAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa > 1:686842/1‑139 (MQ=255) cAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa < 2:676387/139‑1 (MQ=255) ccTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa > 1:933542/1‑131 (MQ=255) ccTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa < 2:933542/131‑1 (MQ=255) ccTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTc > 1:1198914/1‑139 (MQ=255) tGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCaa < 2:686842/139‑1 (MQ=255) gTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATg < 2:1230832/97‑1 (MQ=255) gTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATg > 1:1230832/1‑97 (MQ=255) gTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAg > 2:91261/1‑139 (MQ=255) ttCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGc > 1:518764/1‑139 (MQ=255) cAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCtt < 2:1071788/139‑1 (MQ=255) tCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCaa < 2:552773/139‑1 (MQ=255) cGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACg > 1:4661/1‑102 (MQ=255) cGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACg < 2:4661/102‑1 (MQ=255) gAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAaga < 1:407192/139‑1 (MQ=255) aaaGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa < 2:310841/110‑1 (MQ=255) aaaGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa > 1:310841/1‑110 (MQ=255) aaaGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAagag > 1:960360/1‑139 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTac < 2:1198914/139‑1 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTac < 2:365834/139‑1 (MQ=255) ttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTacaa < 2:1108281/139‑1 (MQ=255) gcgcTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTAcaacaa < 1:91261/139‑1 (MQ=255) gcTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACaaaa > 1:354656/1‑139 (MQ=255) cAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAaga < 1:550851/139‑1 (MQ=255) tcCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATcg < 2:1274486/139‑1 (MQ=255) ccATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTac < 1:1210382/118‑1 (MQ=255) ccATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTac > 2:1210382/1‑118 (MQ=255) ccATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATcgc > 2:801514/1‑139 (MQ=255) gACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCtt > 1:322055/1‑94 (MQ=255) gACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCtt < 2:322055/94‑1 (MQ=255) gACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTc > 1:546413/1‑139 (MQ=255) cAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑c‑c‑ccccc < 2:402395/54‑2 (MQ=255) cAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑c‑c‑ccccc > 1:402395/1‑53 (MQ=255) tAAAAACGGTATCATCGGCAGCTGCATTCAGTGACGCAAACAAGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGc < 1:928322/87‑1 (MQ=255) tAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa < 2:1088828/73‑1 (MQ=255) tAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa > 1:1088828/1‑73 (MQ=255) tAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGc > 2:928322/1‑87 (MQ=255) tAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCa < 1:899876/91‑1 (MQ=255) tAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCa > 2:899876/1‑91 (MQ=255) ggCAGCTGCATTCAGGGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATc < 1:724084/139‑1 (MQ=255) aTTCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAg < 2:354656/139‑1 (MQ=255) aTTCAGTGACGCAAACATG‑C‑CGCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAg < 2:420480/139‑1 (MQ=255) ttCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa < 2:424152/47‑1 (MQ=255) ttCAGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGaa > 1:424152/1‑47 (MQ=255) ctCAGTGACGCAAACATG‑CTC‑CCCCGCCAACATAACGATGGCAGGAACTGGCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGc < 1:1172978/138‑1 (MQ=255) aGTGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTc > 1:1053134/1‑139 (MQ=255) tGACGCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTccc > 1:604433/1‑139 (MQ=255) gCAAACATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGa < 2:731415/139‑1 (MQ=255) aCATGCC‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAgg > 1:1038042/1‑139 (MQ=255) | CTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑C‑C‑CCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGG > NC_000913/1805905‑1806169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |