| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 380,022 | (G)10→11 | intergenic (‑141/+47) | frmR ← / ← yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 380,012 | 1 | . | G | 75.5% | 144.6 / 39.8 | 53 | intergenic (‑131/+57) | frmR/yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (8/5); new base G (29/11); total (37/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGT > NC_000913/379875‑380133 | tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑g > 2:1127017/1‑139 (MQ=255) cTCATCATCTGCATGCAAAATACTATAGGGGGGTATTCTATATGTCAATGCATCCCCCCCTATAGTATATAACACGGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑gggggg‑gg‑gggctaa < 2:550306/139‑6 (MQ=255) tgtaaTTTACTAAAGGGGGGTATTCTATATGTCAATACATACCCCCCTATAGTATATAACACTGGAAAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAgg < 1:462074/136‑1 (MQ=255) tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAgg > 1:1169247/1‑139 (MQ=255) tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAgg > 1:361338/1‑139 (MQ=255) gcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTAGATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGGCGGAAAACAAAGAGG‑A‑AAGGGGGGGG‑GG‑GGCTAATCGGCAGGGAAgg < 1:1127017/139‑1 (MQ=255) taGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑gggggg‑g > 1:741512/1‑106 (MQ=255) taGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAAAAAAGAGG‑A‑AA‑‑gggggg‑g < 2:741512/106‑1 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg > 2:916353/1‑139 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg > 2:18109/1‑139 (MQ=255) ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGCCCGCCCCGGATAgcgggc > 2:1242941/1‑139 (MQ=255) tATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGTA‑AG‑‑GGGGGG‑GG‑GGGTAATGGGGAGGAAGGGCGGCCCCggtaaacggccgg > 1:1155738/1‑128 (MQ=255) tATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg > 2:1247263/1‑139 (MQ=255) tATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg > 2:430147/1‑139 (MQ=255) gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAGGGGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCGGGATAGCGGGCGGCAGAAGGaa > 1:88267/1‑139 (MQ=255) gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAGGGGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa > 1:1050208/1‑139 (MQ=255) cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGAAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg > 1:939144/1‑139 (MQ=255) aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa < 2:939144/139‑1 (MQ=255) tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCGGGGAAGGCCGCCCCGGAAGGGGGGCGGCGGAAGGAATCAGaa > 1:422445/1‑139 (MQ=255) tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa > 2:679550/1‑139 (MQ=255) tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa > 1:296246/1‑139 (MQ=255) tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg > 1:52707/1‑139 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGGCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt > 1:818346/1‑139 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGGGGCAGAGGGAATCAGAATTTCCAGGTCAGACGGGCTGCa > 2:28041/1‑139 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg > 1:978064/1‑129 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGGAAGACGGGCTGCa > 1:455608/1‑139 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GGTGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg < 2:978064/129‑1 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAGGGGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa > 1:561086/1‑139 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa < 1:1253270/128‑1 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa > 2:1253270/1‑128 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg > 2:1060044/1‑139 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAgg > 2:519523/1‑79 (MQ=255) aacTGGAAAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAgg < 1:519523/78‑1 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑ACAG‑‑GGGGGG‑GG‑GGCCAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGGGGCAGAAGGTATCAGAATTTCAAGGTCAGACGGGGTGCAAGTTg > 2:843098/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg > 2:1015694/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGAAAGCGGGCGGCAGGAGGAATAAGAATTTCCAGGTCAGACGGGCGGCAAGTTg > 2:1187234/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg > 2:291080/1‑123 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc > 2:230869/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGAGGTAAGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATAGGCAGGAGAAGCGGGCCCGGATGACGGGCGGCAGAAGGAAAGAAAATTTGAAGGACAGGCgggaggagagaaa > 1:859/1‑129 (MQ=255) tGGAAAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg < 1:291080/123‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGTTTGTTAAAAAAAGAGG‑G‑AA‑‑GGGGGGCGG‑GACCAATCGGCAGGGGATGCTGCCCCGGATAGCGGGCGGCAGAAGGAAACAGAATTTCCAGGTCAGACCGGCTGCAAGTTGCa < 1:843098/139‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa < 1:18109/139‑1 (MQ=255) aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCAAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAGGTTGCAGAc > 1:1267299/1‑139 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg > 1:612548/1‑139 (MQ=255) tttATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa < 1:699175/139‑1 (MQ=255) gTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa > 1:1191209/1‑123 (MQ=255) gTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa < 2:1191209/123‑1 (MQ=255) gTGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCgg > 2:1243323/1‑139 (MQ=255) tGAATGTGGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATC‑GAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGtt > 2:840991/1‑139 (MQ=255) ggAATGTGGGCGGAAAAAAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt < 2:422445/138‑1 (MQ=255) gtgGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg < 2:1169247/139‑1 (MQ=255) gtgGTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg < 1:1243323/139‑1 (MQ=255) gTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa > 2:529666/1‑82 (MQ=255) gTCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa < 1:529666/82‑1 (MQ=255) tCGGAAAACAAAGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCGGCATGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTc > 2:370275/1‑139 (MQ=255) cGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGt > 2:1116330/1‑139 (MQ=255) aaaGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGACCCGGATAGCGGGCGGCAGAAGGAATCAGa > 2:610827/1‑72 (MQ=255) aaaGAGG‑A‑AAG‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGACCCGGATAGCGGGCGGCAGAAGGAATCAGa < 1:610827/72‑1 (MQ=255) | TGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGG‑A‑AA‑‑GGGGGG‑GG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGT > NC_000913/379875‑380133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |