Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,763,869 | (T)7→8 | coding (1285/1617 nt) | yfjL ← | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,763,862 | 1 | . | T | 95.3% | 214.7 / 1.6 | 64 | coding (1292/1617 nt) | yfjL | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
Reads supporting (aligned to +/- strand): ref base . (0/3); new base T (29/32); total (29/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.45e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TAGATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑T‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCAC > NC_000913/2763732‑2763988 | tagATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑t‑tt‑tt‑ttt < 1:896321/139‑2 (MQ=255) tAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTgcgcg < 1:652180/139‑1 (MQ=255) agagTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTgcgcgc > 2:1155939/1‑139 (MQ=255) agTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTgcgcgc < 2:467015/137‑1 (MQ=255) agTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTgcgcgc > 1:467015/1‑137 (MQ=255) agTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑t‑tt‑tt‑tttgc > 2:1126293/1‑130 (MQ=255) agTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑t‑tt‑tt‑tttgc < 1:1126293/133‑4 (MQ=255) tCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTa > 1:353309/1‑139 (MQ=255) tAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCg > 2:253986/1‑139 (MQ=255) cccACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTTAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCTAGGA‑TATT‑TT‑TTGCGCGCGGTATCGATg < 2:816721/139‑1 (MQ=255) cACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCa > 1:169543/1‑139 (MQ=255) cACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGAATCGATGCa > 1:776327/1‑139 (MQ=255) gaacaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGaa > 1:739876/1‑138 (MQ=255) gaacaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGaa < 2:739876/138‑1 (MQ=255) aacaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTgcgcg > 1:882924/1‑117 (MQ=255) aacaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTAt < 1:487148/123‑1 (MQ=255) aacaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTAt > 2:487148/1‑123 (MQ=255) aacaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑T‑TTGTT‑TTgcgcg < 2:882924/117‑1 (MQ=255) acaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATg < 1:551535/139‑1 (MQ=255) caAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGt < 1:253986/139‑1 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCCATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 1:1227105/139‑1 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 1:991167/139‑1 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 2:900721/139‑1 (MQ=255) aCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCt > 1:1184944/1‑139 (MQ=255) tGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGt < 2:555652/139‑1 (MQ=255) aaaCGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTAc < 2:391256/139‑1 (MQ=255) tCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGt > 1:526802/1‑125 (MQ=255) tCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGt < 2:526802/125‑1 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAtct > 2:348927/1‑139 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGAATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAtct > 1:941987/1‑139 (MQ=255) gAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCgg < 2:634670/139‑1 (MQ=255) ttGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCt > 1:639916/1‑139 (MQ=255) ggCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 1:328686/105‑1 (MQ=255) ggCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTcc > 2:328686/1‑105 (MQ=255) tGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACgg < 2:1087977/139‑1 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 2:1087898/139‑1 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 2:1184944/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:754761/139‑1 (MQ=255) tttCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAatat < 1:818224/139‑1 (MQ=255) aTGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCAcc > 1:1316361/1‑139 (MQ=255) gTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCaa < 2:639916/139‑1 (MQ=255) tCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑T‑TT‑TTATTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAAc < 1:934090/139‑1 (MQ=255) tATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTcc > 1:480082/1‑60 (MQ=255) tATCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 2:480082/60‑1 (MQ=255) aTCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAAt > 1:309087/1‑100 (MQ=255) aTCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAAt < 2:309087/100‑1 (MQ=255) aTCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAg > 2:138559/1‑139 (MQ=255) tCGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGc > 2:1070845/1‑139 (MQ=255) cGTCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCt > 1:375859/1‑139 (MQ=255) tCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc > 2:438979/1‑93 (MQ=255) tCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 1:438979/93‑1 (MQ=255) tCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCaaa < 1:513547/134‑1 (MQ=255) tCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCaaa > 2:513547/1‑134 (MQ=255) tCTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGa > 1:60894/1‑139 (MQ=255) cTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGNAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGAt < 2:702822/139‑1 (MQ=255) cTTTAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGAt < 2:941987/139‑1 (MQ=255) tttAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATc < 1:663853/139‑1 (MQ=255) tttAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATc < 2:375859/139‑1 (MQ=255) tAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAAc > 2:399006/1‑60 (MQ=255) tAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAAc < 1:399006/60‑1 (MQ=255) tAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 1:911384/90‑1 (MQ=255) tAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa > 2:911384/1‑90 (MQ=255) tAATGTCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCAc > 2:485902/1‑139 (MQ=255) tCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa > 2:1284583/1‑85 (MQ=255) tCGAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 1:1284583/85‑1 (MQ=255) gAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATc < 2:231533/130‑1 (MQ=255) gAGGATT‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATc > 1:231533/1‑130 (MQ=255) | TAGATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑T‑TT‑TT‑TTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCAC > NC_000913/2763732‑2763988 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |