| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,606,215 | (G)8→9 | noncoding (72/87 nt) | leuP ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,207 | 1 | . | G | 94.4% | 176.3 / 2.9 | 54 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (21/30); total (22/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01 | |||||||||||
AAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC > NC_000913/4606071‑4606341 | aaGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg > 2:846083/1‑139 (MQ=255) aTAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggg < 2:412384/139‑1 (MQ=255) tAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggggg > 2:141333/1‑139 (MQ=255) tAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggggg < 1:1309578/139‑1 (MQ=255) aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggggg > 1:751043/1‑139 (MQ=255) aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACAAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggggg > 2:1281426/1‑139 (MQ=255) aTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggggggg < 1:1232267/139‑1 (MQ=255) tGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggggggga > 1:363202/1‑137 (MQ=255) gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTACCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggggggggactt > 1:476459/1‑133 (MQ=255) cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGa > 1:1016462/1‑139 (MQ=255) cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGa < 1:65933/139‑1 (MQ=255) cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGa > 2:945309/1‑139 (MQ=255) aGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAAc > 1:921957/1‑139 (MQ=255) gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAAccccc < 2:1016462/139‑1 (MQ=255) gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAAccccc < 1:141333/139‑1 (MQ=255) ggACTTGAACCCCCACGTCCGTAAGGACACTAACAACTGAAGCTAGCGCGTCTACCAATTCCGGCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTTGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACg < 1:692247/139‑1 (MQ=25) gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTa > 2:1088031/1‑139 (MQ=255) aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAg < 2:676728/139‑1 (MQ=255) ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGaa < 2:226306/139‑1 (MQ=255) ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCGTCGCACAGTGGTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGaa < 1:654359/139‑1 (MQ=25) aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt < 2:751043/139‑1 (MQ=255) aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCAACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt < 1:498313/139‑1 (MQ=255) gTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa < 2:363202/139‑1 (MQ=255) gTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa < 2:63939/139‑1 (MQ=255) gTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAATCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa < 2:1245663/139‑1 (MQ=255) tCCCTAAGGAAAATAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAATCATTTTACTTTTTTTGGTAATGCCTCGTTTTGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa < 1:1281426/139‑1 (MQ=25) tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt > 2:1100925/1‑139 (MQ=255) acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa > 2:371073/1‑139 (MQ=255) acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGaa < 1:631050/99‑1 (MQ=255) acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGaa > 2:631050/1‑99 (MQ=255) acacCTGAAGCTAACGCGTCAACCGATTCCGCCACGTTCGCACAGTCATCTTACTTGTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg < 1:672951/139‑1 (MQ=25) cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg < 1:846083/139‑1 (MQ=255) ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt > 1:732248/1‑139 (MQ=255) aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa > 2:479758/1‑139 (MQ=255) cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggggg < 2:683025/75‑1 (MQ=255) cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggggg > 1:683025/1‑75 (MQ=255) gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:488294/139‑1 (MQ=255) gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGTTATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa > 1:978138/1‑139 (MQ=255) cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg > 2:136964/1‑119 (MQ=255) cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg < 1:136964/119‑1 (MQ=255) aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 2:996773/139‑1 (MQ=255) ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc < 2:978138/139‑1 (MQ=255) ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc > 1:1034765/1‑139 (MQ=255) ggcACCTTCGCACCGTCAACTTACTTTTTATGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg < 1:1243192/137‑1 (MQ=18) cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat < 1:1088031/139‑1 (MQ=255) gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc < 1:479758/139‑1 (MQ=255) gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc > 1:217867/1‑139 (MQ=255) tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGt > 2:258886/1‑67 (MQ=255) tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGt < 1:258886/67‑1 (MQ=255) tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTCACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc < 2:887713/129‑1 (MQ=255) tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc > 1:887713/1‑129 (MQ=255) cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt > 1:1069713/1‑139 (MQ=255) aCTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt < 1:371073/139‑1 (MQ=255) cTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttttt > 1:705533/1‑139 (MQ=255) cTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttttt > 1:553048/1‑139 (MQ=255) ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAAccccc < 2:128495/50‑1 (MQ=25) ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAAccccc > 1:128495/1‑50 (MQ=25) ttttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg > 1:1043822/1‑81 (MQ=255) ttttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg < 2:1043822/81‑1 (MQ=255) ttttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAACAACGAAGTTTTTgg > 1:311/1‑139 (MQ=18) ttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc < 1:302804/80‑1 (MQ=255) ttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc > 2:302804/1‑80 (MQ=255) cGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc < 2:476459/139‑1 (MQ=255) gTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACtt < 2:1069713/139‑1 (MQ=255) ttGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa < 2:217867/139‑1 (MQ=255) gTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAAccc > 1:445217/1‑139 (MQ=255) tGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc < 2:732248/139‑1 (MQ=255) | AAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC > NC_000913/4606071‑4606341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |