Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 199,912 G→A G662D (GGC→GAC)  bamA → BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913199,9120GA100.0% 301.4 / NA 84G662D (GGC→GAC) bamABamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/49);  total (35/49)

TTATGTGCCGATCGATGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGAAAGACCTGTGTAAATCGGATGATGCTG  >  NC_000913/199778‑200043
                                                                                                                                      |                                                                                                                                   
ttATGTGCCGATCGATGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTc                                                                                                                                 <  1:501022/139‑1 (MQ=255)
 tATGTGCCGATCGATGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTcc                                                                                                                                <  2:117590/139‑1 (MQ=255)
   tgtgCCGATCGATGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAg                                                                                                                              <  2:762773/139‑1 (MQ=255)
   tgtgCCGATCGATGACGATCACAAATCGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTCATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAg                                                                                                                              <  1:836654/139‑1 (MQ=255)
          atcgatGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATa                                                                                                                       >  1:841987/1‑139 (MQ=255)
          atcgatGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATa                                                                                                                       >  1:379474/1‑139 (MQ=255)
                    aTCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTcc                                                                                                             <  2:1118599/139‑1 (MQ=255)
                      cacaAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGa                                                                                                           <  1:1018772/139‑1 (MQ=255)
                       acaAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGaa                                                                                                          <  2:253820/139‑1 (MQ=255)
                               gttgttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGttt                                                                                                  >  1:158843/1‑139 (MQ=255)
                                ttgttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCaa                                                                                                                         >  1:54767/1‑115 (MQ=255)
                                ttgttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCaa                                                                                                                         <  2:54767/115‑1 (MQ=255)
                                ttgttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTa                                                                                                 >  2:22957/1‑139 (MQ=255)
                                   ttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTa                                                                                                 <  2:1214502/136‑1 (MQ=255)
                                   ttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTa                                                                                                 >  1:1214502/1‑136 (MQ=255)
                                   ttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACtt                                                                                              >  1:246944/1‑139 (MQ=255)
                                   ttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACtt                                                                                              >  1:1159978/1‑139 (MQ=255)
                                     cTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTcc                                                                                            >  1:233581/1‑139 (MQ=255)
                                     cTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTcc                                                                                            <  1:351982/139‑1 (MQ=255)
                                     cTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTcc                                                                                            <  1:1229186/139‑1 (MQ=255)
                                     cTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTcc                                                                                            <  2:604436/139‑1 (MQ=255)
                                       caccataaCCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCg                                                                                          >  2:385333/8‑139 (MQ=255)
                                        gggCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGc                                                                                         >  2:95862/1‑139 (MQ=255)
                                           cGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATc                                                                                      <  1:95862/139‑1 (MQ=255)
                                             tACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTc                                                                                                                                 >  2:509447/1‑94 (MQ=255)
                                             tACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTc                                                                                                                                 <  1:509447/94‑1 (MQ=255)
                                             tACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAg                                                                                    <  1:302382/139‑1 (MQ=255)
                                             tACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAg                                                                                    <  2:233581/139‑1 (MQ=255)
                                             tACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAg                                                                                    >  1:1310908/1‑139 (MQ=255)
                                              aCCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAgg                                                                                   <  2:567923/139‑1 (MQ=255)
                                              aCCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAgg                                                                                   <  1:1028574/139‑1 (MQ=255)
                                               cccGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGc                                                                                  <  1:617886/139‑1 (MQ=255)
                                               cccGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGc                                                                                  <  2:158843/139‑1 (MQ=255)
                                               cccGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGc                                                                                  <  2:618934/139‑1 (MQ=255)
                                                ccGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGcc                                                                                 <  1:406784/139‑1 (MQ=255)
                                                  gCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAg                                                                               <  2:1159978/139‑1 (MQ=255)
                                                                gATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTcc                                                                                            >  2:904984/1‑112 (MQ=255)
                                                                gATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTcc                                                                                            <  1:904984/112‑1 (MQ=255)
                                                                gATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAg                                                                               <  2:411583/125‑1 (MQ=255)
                                                                gATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAg                                                                               >  1:411583/1‑125 (MQ=255)
                                                                 aTGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGa                                                                >  1:52189/1‑139 (MQ=255)
                                                                   ggTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACt                                                              <  2:534994/139‑1 (MQ=255)
                                                                   ggTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACt                                                              <  1:403093/139‑1 (MQ=255)
                                                                      ttAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATg                                                           >  1:211562/1‑139 (MQ=255)
                                                                      ttACGCCGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTACGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATg                                                           >  1:1223968/1‑139 (MQ=255)
                                                                        aGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGc                                                                                  <  2:1212781/114‑1 (MQ=255)
                                                                        aGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGc                                                                                  >  1:1212781/1‑114 (MQ=255)
                                                                         ggcggcAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAg                                                                                                                              >  2:669059/1‑69 (MQ=255)
                                                                         ggcggcAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAg                                                                                                                              <  1:669059/69‑1 (MQ=255)
                                                                              cAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGaa                                                   >  1:218467/1‑139 (MQ=255)
                                                                               aaaGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAg                                                                                                                              >  2:187905/1‑63 (MQ=255)
                                                                               aaaGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAg                                                                                                                              <  1:187905/63‑1 (MQ=255)
                                                                               aaaGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACt                                                              <  1:953478/127‑1 (MQ=255)
                                                                               aaaGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACt                                                              >  2:953478/1‑127 (MQ=255)
                                                                                  gagaTGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAAtgtg                                               <  1:341157/139‑1 (MQ=255)
                                                                                  gagaTGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAAtgtg                                               <  1:385333/139‑1 (MQ=255)
                                                                                  gagaTGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAAtgtg                                               <  2:179363/139‑1 (MQ=255)
                                                                                            tCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAgg                                                                                   <  2:1150739/93‑1 (MQ=255)
                                                                                            tCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAgg                                                                                   >  1:1150739/1‑93 (MQ=255)
                                                                                             cTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGcc                                                                                 <  2:772487/94‑1 (MQ=255)
                                                                                             cTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGcc                                                                                 >  1:772487/1‑94 (MQ=255)
                                                                                               aCGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACt                                                              <  2:534181/111‑1 (MQ=255)
                                                                                               aCGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACt                                                              >  1:534181/1‑111 (MQ=255)
                                                                                                cGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGgc                                 >  1:597670/1‑139 (MQ=255)
                                                                                                    aaCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGa                             <  1:928580/139‑1 (MQ=255)
                                                                                                     aCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTc                                                                                                                                 <  1:805019/38‑1 (MQ=255)
                                                                                                     aCTTCTACGCCGGTGGTTCCAGCAACGTGCGTGACTTc                                                                                                                                 >  2:805019/1‑38 (MQ=255)
                                                                                                      cTTCTATGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGaaa                           <  2:218467/139‑1 (MQ=255)
                                                                                                            tGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAg                                                                                                        <  2:274539/56‑1 (MQ=255)
                                                                                                            tGCCGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAg                                                                                                        >  1:274539/1‑56 (MQ=255)
                                                                                                              ccGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGttt                                                                                                  <  1:1128894/60‑1 (MQ=255)
                                                                                                              ccGGTGGTTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGttt                                                                                                  >  2:1128894/1‑60 (MQ=255)
                                                                                                                 gtggtTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGAAAGAc                        >  2:643193/1‑131 (MQ=255)
                                                                                                                 gtggtTCCAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGAAAGAc                        <  1:643193/131‑1 (MQ=255)
                                                                                                                 gtggtTCCAGCACCGAGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCAGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGAAAGACCTGTGTaa                >  1:880398/1‑139 (MQ=255)
                                                                                                                        cAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGc                                                                                  <  2:659696/66‑1 (MQ=255)
                                                                                                                        cAGCACCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGc                                                                                  >  1:659696/1‑66 (MQ=255)
                                                                                                                            aCCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACt                                                              <  2:164632/82‑1 (MQ=255)
                                                                                                                            aCCGTGCGTGACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACt                                                              >  1:164632/1‑82 (MQ=255)
                                                                                                                               gtgcgtgACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGAAAGACCTGTGTAAATCGGATGATGCTg  <  2:716344/139‑1 (MQ=255)
                                                                                                                               gtgcgtgACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGAAAGACCTGTGTAAATCGGATGATGCTg  <  2:246944/139‑1 (MQ=255)
                                                                                                                               gtgcgtgACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGAAAGACCTGTGTAAATCGGATGATGCTg  <  2:364438/139‑1 (MQ=255)
                                                                                                                                tgcgtgACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGa                                                                <  2:147518/76‑1 (MQ=255)
                                                                                                                                tgcgtgACTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGa                                                                >  1:147518/1‑76 (MQ=255)
                                                                                                                                      |                                                                                                                                   
TTATGTGCCGATCGATGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTAATTATGATCCGGACTATGATTACGAATGTGCGACTCAGGACGGCGCGAAAGACCTGTGTAAATCGGATGATGCTG  >  NC_000913/199778‑200043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: