Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,247	G→A	R128C (CGT→TGT)	sapD ←	antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,247	0	G	A	100.0%	196.1 / NA	55	R128C (CGT→TGT)	sapD	antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/26);  total (29/26)

TGCAATGGCTATCATCACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGTTCCTGGAAAATCATCGACACGTTATGGCCAACCAGTTTGCG  >  NC_000913/1353113‑1353379                                                                                                                                       |                                                                                                                                     tGCAATGGCTATCATCACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGc                                                                                                                                  >  2:306907/1‑139 (MQ=255)   cAATGGCTATCATCACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGcca                                                                                                                                >  2:758303/1‑139 (MQ=255)           atcatcACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGc                                                                                                                        >  1:684165/1‑139 (MQ=255)           atcatcACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGc                                                                                                                        >  2:397835/1‑139 (MQ=255)                 aCTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTtat                                                                                                                  <  2:742666/139‑1 (MQ=255)                          cATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAgg                                                                                                         <  2:1173561/139‑1 (MQ=255)                               aCCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTggg                                                                                                    <  2:1019385/139‑1 (MQ=255)                                     ggTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGttt                                                                                              >  2:216613/1‑139 (MQ=255)                                     ggTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGttt                                                                                              >  1:615494/1‑139 (MQ=255)                                           cTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATc                                                                                        >  2:1014172/1‑139 (MQ=255)                                           cTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATc                                                                                        <  2:1053112/139‑1 (MQ=255)                                                aGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTg                                                                                   >  2:1159087/1‑139 (MQ=255)                                                aGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTg                                                                                   >  1:60216/1‑139 (MQ=255)                                                 gggAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTtat                                                                                                                  >  1:362860/1‑107 (MQ=255)                                                 gggAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTtat                                                                                                                  <  2:362860/107‑1 (MQ=255)                                                     aaaCTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCaa                                                                                                                            <  2:799275/93‑1 (MQ=255)                                                     aaaCTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCaa                                                                                                                            >  1:799275/1‑93 (MQ=255)                                                     aaaCTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTggcggc                                                                              >  1:155350/1‑139 (MQ=255)                                                        cTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCca                                                                           <  1:758303/139‑1 (MQ=255)                                                         tGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCcac                                                                          <  2:336330/139‑1 (MQ=255)                                                         tGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCcac                                                                          <  1:306907/139‑1 (MQ=255)                                                          gcgcATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTta                                                                                                                   <  1:1304774/97‑1 (MQ=255)                                                          gcgcATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTta                                                                                                                   >  2:1304774/1‑97 (MQ=255)                                                            gcatcgcatcTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACg                                                                       >  1:259638/1‑139 (MQ=255)                                                                cgcatcTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGt                                                                                                >  1:1146017/1‑110 (MQ=255)                                                                cgcatcTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGt                                                                                                <  2:1146017/110‑1 (MQ=255)                                                                cgcatcTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCcac                                                                          >  2:584631/1‑132 (MQ=255)                                                                cgcatcTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCcac                                                                          <  1:584631/132‑1 (MQ=255)                                                                  catcTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGa                                                                 <  2:259638/139‑1 (MQ=255)                                                                       ttGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGt                                                            <  2:1190333/139‑1 (MQ=255)                                                                        tgtgATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTtat                                                                                                                  >  2:187286/1‑84 (MQ=255)                                                                        tgtgATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTtat                                                                                                                  <  1:187286/84‑1 (MQ=255)                                                                          tgATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGccacca                                                                                                                             <  2:570532/71‑1 (MQ=255)                                                                          tgATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGccacca                                                                                                                             >  1:570532/1‑71 (MQ=255)                                                                                 tAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTta                                                                                                                   <  1:71414/74‑1 (MQ=255)                                                                                 tAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTta                                                                                                                   >  2:71414/1‑74 (MQ=255)                                                                                    tCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTg                                               >  2:387253/1‑139 (MQ=255)                                                                                      cccACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCg                                             <  1:66580/139‑1 (MQ=255)                                                                                       ccgcgtggggcagcaGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCgg                                            >  2:536645/10‑139 (MQ=255)                                                                                         aCGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCcac                                                                          >  1:1152857/1‑107 (MQ=255)                                                                                         aCGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCcac                                                                          <  2:1152857/107‑1 (MQ=255)                                                                                         aCGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGtt                                          >  2:1221601/1‑139 (MQ=255)                                                                                         aCGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGtt                                          >  2:588680/1‑139 (MQ=255)                                                                                          cgcgGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCaa                                                                                                                            >  1:828533/1‑56 (MQ=255)                                                                                          cgcgGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCaa                                                                                                                            <  2:828533/56‑1 (MQ=255)                                                                                                         cAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTggcg                                                                                >  1:742653/1‑85 (MQ=255)                                                                                                         cAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTggcg                                                                                <  2:742653/85‑1 (MQ=255)                                                                                                         cAATGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGTTCCTGGAAAATCATCGa                          <  2:598255/139‑1 (MQ=255)                                                                                                          aaTGGCACGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGTTCCTGGAAAATCATCGac                         <  1:536645/139‑1 (MQ=255)                                                                                                                aCGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGTTCCTGGAAAATCATCGACACGTTa                   <  2:615494/139‑1 (MQ=255)                                                                                                                 cGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGTTCCTGGAAAATCATCGACACGTTAt                  >  2:1158046/1‑139 (MQ=255)                                                                                                                 cGGCGTTTGCGCCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGTTCCTGGAAAATCATCGACACGTTAt                  >  1:1242098/1‑139 (MQ=255)                                                                                                                         gcgcCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGTTCCTGGAAAatcat                             >  2:1186115/1‑120 (MQ=255)                                                                                                                         gcgcCAGCCAAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGTTCCTGGAAAatcat                             <  1:1186115/120‑1 (MQ=255)                                                                                                                                 caAAACACTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGTTCCTGGAAAATCATCGACACGTTATGGCCAACCAGTTTgcg  <  1:1158046/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     TGCAATGGCTATCATCACTTTCTGACATTCACCTTCGGTCAACTCATAGGGAAAACTGCGCATCGCATCTTTGTGATCTTTAATCCCCACGCGGTGCAGCAGTTCAATGGCACGGCGTTTGCGCCAGCCAAAACGCTGCCACCAACGGCCTTTATAGGTCCAGGCTGGGATGTTTTGCATCAACTGGCGGCCCACACGTTCTGAAGGGTCAAGACACGACTGCGGTTCCTGGAAAATCATCGACACGTTATGGCCAACCAGTTTGCG  >  NC_000913/1353113‑1353379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑