| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,364,513 | T→C | R94R (CGT→CGC) | puuB → | gamma‑glutamylputrescine oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,364,513 | 0 | T | C | 95.7% | 159.6 / ‑2.4 | 47 | R94R (CGT→CGC) | puuB | gamma‑glutamylputrescine oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (19/26); total (20/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GGGCTTTGACGTAGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAG > NC_000913/1364378‑1364646 | gggCTTTGACGTAGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATg < 1:285454/139‑1 (MQ=255) tGACGTAGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGgc > 1:23877/1‑139 (MQ=255) gACGTAGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGgca < 1:600638/139‑1 (MQ=255) aGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCatg < 2:964239/139‑1 (MQ=255) aGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCatg > 1:672309/1‑139 (MQ=255) aGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCatg > 1:432545/1‑139 (MQ=255) ctcGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCg > 2:1100880/1‑139 (MQ=255) tcGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGa < 1:1094971/139‑1 (MQ=255) tcGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGa < 2:1153817/139‑1 (MQ=255) gAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAg > 2:175306/1‑139 (MQ=255) gAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAg < 1:332481/139‑1 (MQ=255) aaGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAgg < 2:23877/139‑1 (MQ=255) gCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAggtg < 1:1062415/139‑1 (MQ=255) ccTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAggtgg > 2:1176893/1‑139 (MQ=255) aCGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTgag > 2:374052/1‑139 (MQ=255) cGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTgaga < 1:1100880/139‑1 (MQ=255) gCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGat < 2:1284580/139‑1 (MQ=255) tCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGatcat > 1:90701/1‑139 (MQ=255) cTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCcgc > 1:1171821/1‑139 (MQ=255) tGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGaa < 1:734964/139‑1 (MQ=255) tGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGaa > 2:208631/1‑139 (MQ=255) gcAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATC‑TCCGCGAACGTAt < 1:374052/139‑1 (MQ=255) aGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCa < 2:548959/139‑1 (MQ=255) cGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCaaa < 1:1176893/139‑1 (MQ=255) cGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCaaa < 1:675248/139‑1 (MQ=255) cGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCaaa < 1:841849/139‑1 (MQ=255) cGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCaaa < 2:672309/139‑1 (MQ=255) cTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTAt > 2:178517/1‑116 (MQ=255) cTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTAt < 1:178517/116‑1 (MQ=255) ccTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGccc > 2:180669/1‑139 (MQ=255) ccTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGccc > 2:629053/1‑139 (MQ=255) aCAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCcgg < 2:1171821/139‑1 (MQ=255) cgcgACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAGGGTGGCGAGATCATCCGCGAACGTATCAAACGCTATCAGATTGACTGCGACTACCGCCCCGGCGGcc > 2:853658/1‑139 (MQ=255) gACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGgcagca > 2:12139/1‑58 (MQ=255) gACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGgcagca < 1:12139/58‑1 (MQ=255) gACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGt > 2:764913/1‑139 (MQ=255) aCATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGtt > 1:1130500/1‑139 (MQ=255) cATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGttt < 2:432545/139‑1 (MQ=255) tGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGat < 1:208631/139‑1 (MQ=255) cTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAg < 1:764913/139‑1 (MQ=255) ggCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAgg < 1:485465/44‑1 (MQ=255) ggCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAgg > 2:485465/1‑44 (MQ=255) gCATGGACACCGCCAGCATGGTCGGCAGCATGATGTTCGAAGGTGGTGAGATCACCCGCGAACGTATCTAACGTTATCGGATTGACTGCGGCTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATGAACAgcgcgc < 1:478169/139‑1 (MQ=255) gACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGatgaa < 1:1117552/119‑1 (MQ=255) gACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGatgaa > 2:1117552/1‑119 (MQ=255) cGCCCGTATGCTCGGCAGCATGATGTTCGAGGGTGGCGAGATCATCCGCGAACGTATCAAACGCTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTCGaa < 1:853658/139‑1 (MQ=255) gCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAag > 2:466197/1‑139 (MQ=255) | GGGCTTTGACGTAGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAG > NC_000913/1364378‑1364646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |