Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,616,355	T→C	V198A (GTC→GCC)	yneK →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,616,355	0	T	C	100.0%	295.8 / NA	78	V198A (GTC→GCC)	yneK	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/34);  total (44/34)

CAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGTCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAA  >  NC_000913/1616237‑1616489                                                                                                                       |                                                                                                                                         cAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTcgcg                                                                                                                      >  1:864227/1‑139 (MQ=255) cAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTcgcg                                                                                                                      <  1:266343/139‑1 (MQ=255) cAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTcgcg                                                                                                                      >  2:1012680/1‑139 (MQ=255)    tttCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTa                                                                                                                   >  1:421916/1‑139 (MQ=255)            gATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTc                                                                                                           >  1:989321/1‑139 (MQ=255)             aTCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCa                                                                                                          <  2:421916/139‑1 (MQ=255)               cAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATa                                                                                                        <  2:726635/139‑1 (MQ=255)                       gtgtTCCCATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCg                                                                                                >  2:164524/1‑139 (MQ=255)                            cccATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTccccc                                                                                                 <  1:418739/133‑1 (MQ=255)                            cccATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTccccc                                                                                                 >  2:418739/1‑133 (MQ=255)                            cccATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTAt                                                                                           >  1:1000834/1‑139 (MQ=255)                            cccATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTAt                                                                                           >  2:463615/1‑139 (MQ=255)                            cccATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGACTAt                                                                                           >  1:273546/1‑139 (MQ=255)                               aTCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGa                                                                                        >  1:641100/1‑139 (MQ=255)                                tCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGaa                                                                                       >  2:1220652/1‑139 (MQ=255)                                tCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGaa                                                                                       <  2:307713/139‑1 (MQ=255)                                tCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGaa                                                                                       <  1:1012680/139‑1 (MQ=255)                                    aaGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑c                                                                                 >  1:461002/1‑139 (MQ=255)                                         cgcATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTg                                                                             <  2:155534/138‑1 (MQ=255)                                         cgcATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTg                                                                             >  1:155534/1‑138 (MQ=255)                                           cATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAgg                                                                          >  1:251066/1‑139 (MQ=255)                                            aTACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGt                                                                         >  1:949460/1‑139 (MQ=255)                                             tACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGtt                                                                        <  2:251066/139‑1 (MQ=255)                                                 ccacATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTcaca                                                                    >  2:971503/1‑139 (MQ=255)                                                   acaTATTGAACCCCTGCCTTCGGATCACCATACCACTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGa                                                                  >  1:308288/1‑139 (MQ=255)                                                         tGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTc                                                            >  2:609452/1‑139 (MQ=255)                                                         tGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTc                                                            <  1:1220652/139‑1 (MQ=255)                                                         tGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTc                                                            >  2:547006/1‑139 (MQ=255)                                                           aaCCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTcccc                                                                                                  >  1:68697/1‑101 (MQ=255)                                                           aaCCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTcccc                                                                                                  <  2:68697/101‑1 (MQ=255)                                                            aCCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGaa                                                                                       <  2:380708/111‑1 (MQ=255)                                                            aCCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGaa                                                                                       >  1:380708/1‑111 (MQ=255)                                                            aCCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTc                                                         >  1:91729/1‑139 (MQ=255)                                                              cccTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTCTGAGGTTCACAGATGGTTCGTc                                                         >  2:748382/1‑139 (MQ=255)                                                               ccTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTcc                                                                                                    <  2:274502/95‑1 (MQ=255)                                                               ccTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTcc                                                                                                    >  1:274502/1‑95 (MQ=255)                                                                cTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTAtctct                                                                                                                          <  1:945492/72‑1 (MQ=255)                                                                cTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTAtctct                                                                                                                          >  2:945492/1‑72 (MQ=255)                                                                cTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACt                                                                                                      >  2:637542/1‑92 (MQ=255)                                                                cTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACt                                                                                                      <  1:637542/92‑1 (MQ=255)                                                                cTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATa                                                     >  1:60782/1‑139 (MQ=255)                                                                 tGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTAtc                                                                                                                             >  1:926225/1‑68 (MQ=255)                                                                 tGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTAtc                                                                                                                             <  2:926225/68‑1 (MQ=255)                                                                 tGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATaa                                                    <  2:60782/139‑1 (MQ=255)                                                                  gCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATa                                                                                                        >  2:793575/1‑88 (MQ=255)                                                                  gCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATa                                                                                                        <  1:793575/88‑1 (MQ=255)                                                                      tCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTTCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATc                                               <  2:1144484/139‑1 (MQ=255)                                                                      tCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATc                                               <  2:1000834/139‑1 (MQ=255)                                                                                aTACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGAt                                     <  2:691844/139‑1 (MQ=255)                                                                                  aCCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAAc                                          <  2:981307/132‑1 (MQ=255)                                                                                  aCCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAAc                                          >  1:981307/1‑132 (MQ=255)                                                                                    cGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGt                                                                                                                    >  2:165858/1‑58 (MQ=255)                                                                                    cGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGt                                                                                                                    <  1:165858/58‑1 (MQ=255)                                                                                     gCTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCg                                <  2:949460/139‑1 (MQ=255)                                                                                      cTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGTTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATaa                                                    <  1:858560/118‑1 (MQ=255)                                                                                      cTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTccccc                                                                                                 <  2:1054502/75‑1 (MQ=255)                                                                                      cTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTccccc                                                                                                 >  1:1054502/1‑75 (MQ=255)                                                                                      cTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGt                                                          >  2:58095/1‑112 (MQ=255)                                                                                      cTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGt                                                          <  1:58095/112‑1 (MQ=255)                                                                                      cTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATaa                                                    >  2:858560/1‑118 (MQ=255)                                                                                      cTGCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGc                               >  1:552262/1‑139 (MQ=255)                                                                                        gCAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTCATTTTGTCATACTCCCCCGATTATGGAATTA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAGAGATCCTAACTTGATCACCGCCa                             >  1:866851/1‑139 (MQ=255)                                                                                         cAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCAt                            >  1:903351/1‑139 (MQ=255)                                                                                         cAGATTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCAt                            >  1:622155/1‑139 (MQ=255)                                                                                            aTTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGaa                                                                                       >  1:1117859/1‑79 (MQ=255)                                                                                            aTTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGaa                                                                                       <  2:1117859/79‑1 (MQ=255)                                                                                            aTTACTTACGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATtct                         <  2:641100/139‑1 (MQ=255)                                                                                              tacttacGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCg                       <  2:461002/139‑1 (MQ=255)                                                                                                   acGCCAGTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATa                  >  2:268992/1‑139 (MQ=255)                                                                                                         gTTTGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGAc            <  1:609452/139‑1 (MQ=255)                                                                                                          tttGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTcccc                                                                                                  >  1:833871/1‑54 (MQ=255)                                                                                                          tttGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTcccc                                                                                                  <  2:833871/54‑1 (MQ=255)                                                                                                          tttGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGaa                                                                                       >  1:585619/1‑65 (MQ=255)                                                                                                          tttGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGaa                                                                                       <  2:585619/65‑1 (MQ=255)                                                                                                          tttGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGtt                                                             <  2:415787/89‑1 (MQ=255)                                                                                                          tttGATTTGCTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGtt                                                             >  1:415787/1‑89 (MQ=255)                                                                                                                   cTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTaa  >  1:392072/1‑139 (MQ=255)                                                                                                                   cTTGCCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTaa  <  1:164524/139‑1 (MQ=255)                                                                                                                       |                                                                                                                                         CAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCCCTGCCTTCGGATCACCATACCGCTGCAGATTACTTACGCCAGTTTGATTTGCTTGTCCTGAATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAA‑‑CTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAA  >  NC_000913/1616237‑1616489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑