Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,625,476	T→C	L635L (TTA→TTG)	dcp ←	dipeptidyl carboxypeptidase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,625,476	0	T	C	100.0%	238.8 / NA	63	L635L (TTA→TTG)	dcp	dipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/33);  total (30/33)

GTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAACTGCTGCGATAGCGTGGCGGTATA  >  NC_000913/1625341‑1625611                                                                                                                                        |                                                                                                                                        gTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAAt                                                                                                                                      >  2:632674/1‑139 (MQ=255)  ttCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATc                                                                                                                                     >  1:910147/1‑139 (MQ=255)  ttCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATc                                                                                                                                     >  1:267804/1‑139 (MQ=255)    cAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGTGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCg                                                                                                                                   >  1:105550/1‑139 (MQ=255)    cAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCg                                                                                                                                   <  2:910147/139‑1 (MQ=255)    cAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCg                                                                                                                                   >  1:1110481/1‑139 (MQ=255)    cAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCg                                                                                                                                   <  2:668223/139‑1 (MQ=255)      aGCAACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGcc                                                                                                                                 <  2:853944/139‑1 (MQ=255)       gCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGccc                                                                                                                                <  2:267804/139‑1 (MQ=255)          aCGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTg                                                                                                                              <  1:1148371/138‑1 (MQ=255)          aCGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTg                                                                                                                              >  2:1148371/1‑138 (MQ=255)              tGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTcaa                                                                                                                         <  1:73153/139‑1 (MQ=255)                   cagcaTCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAAc                                                                                                                    <  2:915464/139‑1 (MQ=255)                   cagcaTCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAAc                                                                                                                    <  1:813401/139‑1 (MQ=255)                   cagcaTCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAAc                                                                                                                    >  2:680150/1‑139 (MQ=255)                                  cTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACcatca                                                                                                     <  1:680150/139‑1 (MQ=255)                                          ccTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCaa                                                                                             >  1:1020635/1‑139 (MQ=255)                                          ccTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCaa                                                                                             >  2:902148/1‑139 (MQ=255)                                           cTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGcc                                                                                                                                 >  1:1261315/1‑102 (MQ=255)                                           cTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGcc                                                                                                                                 <  2:1261315/102‑1 (MQ=255)                                           cTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAAc                                                                                            >  1:508293/1‑139 (MQ=255)                                            ttACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACa                                                                                           >  1:556635/1‑139 (MQ=255)                                             tACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACcatcat                                                                                                    >  2:1176373/1‑129 (MQ=255)                                             tACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACcatcat                                                                                                    <  1:1176373/129‑1 (MQ=255)                                                    ccATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGc                                                                                                >  2:357514/1‑126 (MQ=255)                                                    ccATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGc                                                                                                <  1:357514/126‑1 (MQ=255)                                                     cATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTcc                                                                                  >  1:1157444/1‑139 (MQ=255)                                                          tCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGa                                                                             <  2:105550/139‑1 (MQ=255)                                                           cGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGAt                                                                            <  1:552051/139‑1 (MQ=255)                                                           cGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGAt                                                                            <  1:517920/139‑1 (MQ=255)                                                                        ccAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATaa                                                                          <  2:852857/128‑1 (MQ=255)                                                                        ccAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATaa                                                                          >  1:852857/1‑128 (MQ=255)                                                                        ccAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAAcc                                                               >  1:1309123/1‑139 (MQ=255)                                                                          aGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTg                                                             <  1:389393/139‑1 (MQ=255)                                                                                 cGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCAGTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCata                                                      >  1:797660/1‑139 (MQ=255)                                                                                        aCCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTg                                                                                       <  2:854135/99‑1 (MQ=255)                                                                                        aCCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTg                                                                                       >  1:854135/1‑99 (MQ=255)                                                                                          ctctGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGccacc                                             <  2:511892/139‑1 (MQ=255)                                                                                                aaaGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAat                                       <  2:1020635/139‑1 (MQ=255)                                                                                                    gATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGcc                                                                                                                                 >  2:610001/1‑45 (MQ=255)                                                                                                    gATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGcc                                                                                                                                 <  1:610001/45‑1 (MQ=255)                                                                                                    gATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGc                                                 >  1:4170/1‑125 (MQ=255)                                                                                                    gATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGc                                                 <  2:4170/125‑1 (MQ=255)                                                                                                    gATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAata                                      <  1:359799/136‑1 (MQ=255)                                                                                                    gATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAata                                      >  2:359799/1‑136 (MQ=255)                                                                                                    gATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATgg                                   >  1:1132016/1‑139 (MQ=255)                                                                                                    gATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTACTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATgg                                   >  1:92639/1‑139 (MQ=255)                                                                                                     aTCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATggg                                  <  2:508293/139‑1 (MQ=255)                                                                                                     aTCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATggg                                  <  1:308946/139‑1 (MQ=255)                                                                                                     aTCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATggg                                  <  1:73961/139‑1 (MQ=255)                                                                                                       cGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGc                                                                                                >  2:866935/1‑75 (MQ=255)                                                                                                       cGCCTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGc                                                                                                <  1:866935/75‑1 (MQ=255)                                                                                                         ccTCGCGAAAACGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGaa                              >  1:412640/1‑139 (MQ=255)                                                                                                                aaaaCGCAGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAActg                       <  2:556635/139‑1 (MQ=255)                                                                                                                       aGCCCATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAACTGCTGCGAt                >  1:1070668/1‑139 (MQ=255)                                                                                                                         cccATTTTCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAACTGCTGCGATAg              <  2:1157444/139‑1 (MQ=255)                                                                                                                              tttCACGCGTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAACTGCTGCGATAGCGTgg         <  1:4656/139‑1 (MQ=255)                                                                                                                                   cgcgTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGTAATAACTGCTGCGATAGCGTGGCg       <  2:804743/136‑1 (MQ=255)                                                                                                                                   cgcgTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAAc                         <  2:481365/118‑1 (MQ=255)                                                                                                                                   cgcgTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAAc                         >  1:481365/1‑118 (MQ=255)                                                                                                                                   cgcgTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAACTGCTGCGATAGCGTGGCg       >  1:804743/1‑136 (MQ=255)                                                                                                                                    gcgTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAACTGCTGCGATAGCGTGGCGGtat   <  2:797660/139‑1 (MQ=255)                                                                                                                                     cgTCAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAACTGCTGCGATAGCGTGGCGGtata  >  1:1024609/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        GTTCAAGCCACGATGTTGCAGCATCGGCATAATCTTAGGTGCCTTACCGCGCCATTGTCGATACAGGCGTTCCAGATCTTCGCTGTTACCTCTGGAAAGGATCGCCTCGCGAAAACGCAGCCCATTTTCACGCGTTAATCCGCCCTGCTCAACAAACCACTGATAACCATCATCGGCCAACATTTGCGTCCACAGATAAGCGTAATAACCTGCAGCATATCCGCCACCAAAAATATGGGCGAAATAACTGCTGCGATAGCGTGGCGGTATA  >  NC_000913/1625341‑1625611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑