Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,675,350	A→G	G337G (GGT→GGC)	pntB ←	pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,675,350	0	A	G	100.0%	167.7 / NA	45	G337G (GGT→GGC)	pntB	pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/19);  total (26/19)

TCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCT  >  NC_000913/1675219‑1675470                                                                                                                                    |                                                                                                                         tcAGCAAAGTCATCATTTATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAg                                                                                                                   <  1:240568/139‑1 (MQ=255)   aGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAgcg                                                                                                                 >  2:1210679/1‑139 (MQ=255)   aGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAgcg                                                                                                                 <  2:122019/139‑1 (MQ=255)        aGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCaa                                                                                                            >  2:165082/1‑139 (MQ=255)           catcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAAttt                                                                                                         >  2:598884/1‑139 (MQ=255)           catcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAAttt                                                                                                         <  1:1210679/139‑1 (MQ=255)                      tcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAAttt                                                                                              <  1:299533/139‑1 (MQ=255)                        gTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCa                                                                                            >  2:965203/1‑139 (MQ=255)                        gTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCa                                                                                            >  1:1056806/1‑139 (MQ=255)                        gTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCa                                                                                            >  2:512583/1‑139 (MQ=255)                        gTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCa                                                                                            >  2:387707/1‑139 (MQ=255)                            aTTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGa                                                                                        <  1:1299768/139‑1 (MQ=255)                                   gCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCg                                                                                         >  1:1185899/1‑131 (MQ=255)                                   gCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCg                                                                                         <  2:1185899/131‑1 (MQ=255)                                         tGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                           >  2:162534/1‑123 (MQ=255)                                         tGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                           <  1:162534/123‑1 (MQ=255)                                               aCGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcgc                                                                     <  1:165082/139‑1 (MQ=255)                                                  gTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAACCGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGAc                                                                  >  1:709651/1‑139 (MQ=255)                                                   tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                           >  1:527029/1‑113 (MQ=255)                                                   tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                           <  2:527029/113‑1 (MQ=255)                                                   tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                 >  2:1074366/1‑139 (MQ=255)                                                     cTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGTCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGc                                                               >  2:1122158/1‑139 (MQ=255)                                                             tCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                       >  1:221135/1‑139 (MQ=255)                                                             tCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                       >  2:165851/1‑139 (MQ=255)                                                               agccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAc                                                     >  2:423105/1‑139 (MQ=255)                                                               agccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAc                                                     >  1:657773/1‑139 (MQ=255)                                                                             tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGAt                                       <  1:423105/139‑1 (MQ=255)                                                                               atatGTCCAGGCAAACGCCCCGCGACCGGGTGGATTCCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAccc                                                   <  1:953490/125‑1 (MQ=255)                                                                               atatGTCCAGGCAAACGCCCCGCGACCGGGTGGATTCCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAccc                                                   >  2:953490/1‑125 (MQ=255)                                                                                     ccAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAg                                                                                                                   <  2:711537/55‑1 (MQ=255)                                                                                     ccAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAg                                                                                                                   >  1:711537/1‑55 (MQ=255)                                                                                         gCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                 <  1:415792/117‑1 (MQ=255)                                                                                         gCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                 >  2:415792/1‑117 (MQ=255)                                                                                              cGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtttt                      >  2:725800/1‑139 (MQ=255)                                                                                              cGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtttt                      >  2:1060772/1‑139 (MQ=255)                                                                                               gCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttttt                     <  1:165851/139‑1 (MQ=255)                                                                                                              ggATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGt      <  1:1074366/139‑1 (MQ=255)                                                                                                                aTACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtct    <  1:725800/139‑1 (MQ=255)                                                                                                                  aCCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTctct  >  2:866408/1‑139 (MQ=255)                                                                                                                      aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                 <  2:1274116/88‑1 (MQ=255)                                                                                                                      aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                 >  1:1274116/1‑88 (MQ=255)                                                                                                                           cacaTTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                           <  1:837848/41‑1 (MQ=255)                                                                                                                           cacaTTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAccc                                                   >  1:748254/1‑81 (MQ=255)                                                                                                                           cacaTTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAccc                                                   <  2:748254/81‑1 (MQ=255)                                                                                                                           cacaTTAATGCCACGAGCGCGCAATTTCTCAGTAATGTCAg                                                                                           >  2:837848/1‑41 (MQ=255)                                                                                                                                    |                                                                                                                         TCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCT  >  NC_000913/1675219‑1675470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑