Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,240,094 T→C A85A (GCA→GCG)  mglB ← methyl‑galactoside transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,240,0940TC100.0% 243.1 / NA 64A85A (GCA→GCG) mglBmethyl‑galactoside transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/30);  total (34/30)

GTAGGCTTTGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGTGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACA  >  NC_000913/2239962‑2240226
                                                                                                                                    |                                                                                                                                    
gtagGCTTTGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTc                                                                                                                                >  2:1165760/1‑139 (MQ=255)
    gCTTTGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCAccc                                                                                                                            >  1:107471/1‑139 (MQ=255)
        tGTCGTAGCTATCCAGCGCCTTACGAGATGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCttt                                                                                                                        >  2:132027/1‑139 (MQ=255)
        tGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCttt                                                                                                                        >  2:581069/1‑139 (MQ=255)
         gTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTc                                                                                                                       <  2:431367/139‑1 (MQ=255)
                cTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCa                                                                                                                <  2:107471/139‑1 (MQ=255)
                   tCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATa                                                                                                             >  1:769261/1‑139 (MQ=255)
                      aGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGt                                                                                                          <  1:909099/139‑1 (MQ=255)
                          ccTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGAt                                                                                                      >  2:405882/1‑139 (MQ=255)
                          ccTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGAt                                                                                                      >  2:1199044/1‑139 (MQ=255)
                                  gaCGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGt                                                                                              <  1:1144532/139‑1 (MQ=255)
                                  gaCGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGt                                                                                              <  1:581069/139‑1 (MQ=255)
                                     ggTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGa                                                                                                       >  1:1263516/1‑127 (MQ=255)
                                     ggTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGa                                                                                                       <  2:1263516/127‑1 (MQ=255)
                                           ttgttgAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTgg                                                                                     <  1:405882/139‑1 (MQ=255)
                                           ttgttgAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTgg                                                                                     <  2:802954/139‑1 (MQ=255)
                                            tgttgAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGa                                                                                    >  2:1007766/1‑139 (MQ=255)
                                                 aagaaAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGc                                                                                         >  1:359279/1‑129 (MQ=255)
                                                 aagaaAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGc                                                                                         <  2:359279/129‑1 (MQ=255)
                                                 aagaaAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGt                                                                               <  1:132027/139‑1 (MQ=255)
                                                           ccGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTgaga                                                                     >  1:713663/1‑139 (MQ=255)
                                                             ggCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAAt                                                                   <  2:769261/139‑1 (MQ=255)
                                                                       gCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATcag                                                         <  1:658124/139‑1 (MQ=255)
                                                                             gcgcTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTg                                                   <  2:725172/139‑1 (MQ=255)
                                                                                         tCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGtt                                                                                             >  2:1304678/1‑85 (MQ=255)
                                                                                         tCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGtt                                                                                             <  1:1304678/85‑1 (MQ=255)
                                                                                           aCCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCgg                                     <  1:63628/139‑1 (MQ=255)
                                                                                             cGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCt                                   >  1:1126473/1‑139 (MQ=255)
                                                                                             cGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCt                                   >  2:453285/1‑139 (MQ=255)
                                                                                             cGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCt                                   >  1:65288/1‑139 (MQ=255)
                                                                                              gTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCtt                                  >  2:819958/1‑139 (MQ=255)
                                                                                               tACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCa                                                                                                                <  1:828485/60‑1 (MQ=255)
                                                                                               tACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCa                                                                                                                >  2:828485/1‑60 (MQ=255)
                                                                                               tACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCttt                                 >  1:376361/1‑139 (MQ=255)
                                                                                               tACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCttt                                 >  2:1223933/1‑139 (MQ=255)
                                                                                               tACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCttt                                 >  2:1166269/1‑139 (MQ=255)
                                                                                                aCCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCAGCAGCAGCTGAACATCTGGCGCGGCTTTc                                <  2:78172/139‑1 (MQ=255)
                                                                                                 cccGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCGCCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCg                               >  2:859625/1‑139 (MQ=255)
                                                                                                 cccGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACg                                                                                                           <  2:284730/63‑1 (MQ=255)
                                                                                                 cccGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACg                                                                                                           >  1:284730/1‑63 (MQ=255)
                                                                                                 cccGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATtcatc                                                           <  2:463394/111‑1 (MQ=255)
                                                                                                 cccGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATtcatc                                                           >  1:463394/1‑111 (MQ=255)
                                                                                                     cAGCTGCCGGGTCAATCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGt                                                                               <  1:1239233/87‑1 (MQ=255)
                                                                                                     cAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGt                                                                               >  2:1239233/1‑87 (MQ=255)
                                                                                                       gCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCtt                         <  1:819958/139‑1 (MQ=255)
                                                                                                       gCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCtt                         >  2:707771/1‑139 (MQ=255)
                                                                                                         tGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGTGCGGCTTTCGCATCTTGc                       >  1:463269/1‑139 (MQ=255)
                                                                                                          gCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCt                      >  2:112744/1‑139 (MQ=255)
                                                                                                           ccGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATtcatc                                                           <  1:1269550/101‑1 (MQ=255)
                                                                                                           ccGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATtcatc                                                           >  2:1269550/1‑101 (MQ=255)
                                                                                                           ccGGGTCAACCAGGTCGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTc                     >  1:879634/1‑139 (MQ=255)
                                                                                                            cGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGc                                                                                                                     <  2:58832/42‑1 (MQ=255)
                                                                                                            cGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGc                                                                                                                     >  1:58832/1‑42 (MQ=255)
                                                                                                             gggTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCaa                   <  1:255367/139‑1 (MQ=255)
                                                                                                                  aaCCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGcc              <  2:376361/139‑1 (MQ=255)
                                                                                                                  aaCCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGcc              <  1:379100/139‑1 (MQ=255)
                                                                                                                       ggTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTgcg         <  1:1270842/139‑1 (MQ=255)
                                                                                                                         ttGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCa       <  1:1199044/139‑1 (MQ=255)
                                                                                                                            aTTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTg                        <  1:1242005/119‑1 (MQ=255)
                                                                                                                            aTTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTg                        >  2:1242005/1‑119 (MQ=255)
                                                                                                                             ttGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTAc   >  1:664602/1‑139 (MQ=255)
                                                                                                                              tGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACa  >  2:497853/1‑139 (MQ=255)
                                                                                                                              tGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACa  >  1:1221767/1‑139 (MQ=255)
                                                                                                                              tGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACa  <  1:497853/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
GTAGGCTTTGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGTGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACA  >  NC_000913/2239962‑2240226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑