Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,445,253 T→C Y103C (TAC→TGC)  yfcA ← TauE/TSUP family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,445,2530TC100.0% 264.5 / NA 72Y103C (TAC→TGC) yfcATauE/TSUP family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/39);  total (33/39)

ACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTG  >  NC_000913/2445125‑2445383
                                                                                                                                |                                                                                                                                  
aCGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTGAGACATCAGCCTGAACGCATTGCACcag                                                                                                                          <  2:814401/139‑1 (MQ=255)
     aaGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTg                                                                                                                     >  2:189650/1‑139 (MQ=255)
       gggTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTgcg                                                                                                                   <  1:189650/139‑1 (MQ=255)
              ccGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCa                                                                                                            <  1:470962/139‑1 (MQ=255)
               cGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCAt                                                                                                           <  1:300343/139‑1 (MQ=255)
                  aCATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGa                                                                                                        >  2:9902/1‑139 (MQ=255)
                   cATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAAATACCAGAATGGGCAAAATCTGCCGTAAGACATCCGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAg                                                                                                       <  1:494343/139‑1 (MQ=255)
                          cgcTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACaa                                                                                                >  2:678798/1‑139 (MQ=255)
                              ggCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGt                                                                                            >  1:571612/1‑139 (MQ=255)
                                cGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCa                                                                                          <  2:93485/139‑1 (MQ=255)
                                   tcttctTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATgg                                                                                       <  2:1004300/139‑1 (MQ=255)
                                        ttcACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGata                                                                                  <  1:151599/139‑1 (MQ=255)
                                             cTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAg                                                                             >  2:376011/1‑139 (MQ=255)
                                               aGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAg                                                                                                       <  2:239553/111‑1 (MQ=255)
                                               aGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAg                                                                                                       >  1:239553/1‑111 (MQ=255)
                                               aGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGtt                                                                           >  2:499228/1‑139 (MQ=255)
                                                 cTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTc                                                                         >  1:438246/1‑139 (MQ=255)
                                                   ttGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTg                                                                       <  2:1031768/139‑1 (MQ=255)
                                                        aTCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGc                                                                  <  2:489528/139‑1 (MQ=255)
                                                        aTCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGc                                                                  <  2:571612/139‑1 (MQ=255)
                                                           aGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTa                                                               >  1:573162/1‑139 (MQ=255)
                                                                     agagGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCg                                                                                     >  2:660743/1‑107 (MQ=255)
                                                                     agagGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCg                                                                                     <  1:660743/107‑1 (MQ=255)
                                                                       agGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGcc                                                                                                     <  1:491394/89‑1 (MQ=255)
                                                                       agGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGcc                                                                                                     >  2:491394/1‑89 (MQ=255)
                                                                       agGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGc                                                                  <  2:555630/124‑1 (MQ=255)
                                                                       agGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGc                                                                  >  1:555630/1‑124 (MQ=255)
                                                                        ggCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACttt                                                  >  2:265905/1‑139 (MQ=255)
                                                                         gCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAg                                                                                                       <  2:1091798/85‑1 (MQ=255)
                                                                         gCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAg                                                                                                       >  1:1091798/1‑85 (MQ=255)
                                                                          ccGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTgc                                                <  2:667261/139‑1 (MQ=255)
                                                                           cGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAg                                                                                                       >  2:41227/1‑83 (MQ=255)
                                                                           cGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAg                                                                                                       <  1:41227/83‑1 (MQ=255)
                                                                           cGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGc                                                                  >  1:256661/1‑120 (MQ=255)
                                                                           cGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGc                                                                  <  2:256661/120‑1 (MQ=255)
                                                                           cGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTgcg                                               <  2:438246/139‑1 (MQ=255)
                                                                           cGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTgcg                                               >  2:771174/1‑139 (MQ=255)
                                                                            gATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCa                                                                                          <  1:252854/95‑1 (MQ=255)
                                                                            gATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCa                                                                                          >  2:252854/1‑95 (MQ=255)
                                                                            gATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCt                                                                 >  2:435945/1‑120 (MQ=255)
                                                                            gATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCt                                                                 <  1:435945/120‑1 (MQ=255)
                                                                            gATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTgcgg                                              >  1:1211153/1‑139 (MQ=255)
                                                                              tACAAATTACCAGAATGGTCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTgcggcg                                            >  1:732351/1‑139 (MQ=255)
                                                                               aCAAATTACCAGAATGGGCAAAGTCTGCCGTAAGACACCTGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGa                                           <  1:678798/139‑1 (MQ=255)
                                                                               aCAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGa                                           <  2:470737/139‑1 (MQ=255)
                                                                                  aaTTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATa                                        <  2:732351/139‑1 (MQ=255)
                                                                                    ttACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATaaa                                      <  2:573162/139‑1 (MQ=255)
                                                                                           aaTGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGAta                               >  2:1016200/1‑139 (MQ=255)
                                                                                           aaTGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGAta                               >  2:379916/1‑139 (MQ=255)
                                                                                             tGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGAtagt                             <  1:9902/139‑1 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTgcgc                                                                                                                  >  2:30917/1‑52 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTgcgc                                                                                                                  <  1:30917/52‑1 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTgc                                                                                                                    <  2:1181942/50‑1 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTgc                                                                                                                    >  1:1181942/1‑50 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACa                                                                                                 <  1:660567/69‑1 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACa                                                                                                 >  2:660567/1‑69 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACa                                                                                                 >  2:631536/1‑69 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACa                                                                                                 <  1:631536/69‑1 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTg                                                                       >  2:1081995/1‑95 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTg                                                                       <  1:1081995/95‑1 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATa                                        >  1:791912/1‑126 (MQ=255)
                                                                                               ggCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATa                                        <  2:791912/126‑1 (MQ=255)
                                                                                                         gCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACt                                                           <  2:718238/97‑1 (MQ=255)
                                                                                                         gCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACt                                                           >  1:718238/1‑97 (MQ=255)
                                                                                                         gCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATaga                 <  1:379916/139‑1 (MQ=255)
                                                                                                                        ccTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATa                                        <  2:49586/101‑1 (MQ=255)
                                                                                                                        ccTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATa                                        >  1:49586/1‑101 (MQ=255)
                                                                                                                        ccTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTg  <  1:265905/139‑1 (MQ=255)
                                                                                                                          tGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCtt                                                                <  1:999565/75‑1 (MQ=255)
                                                                                                                          tGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCtt                                                                >  2:999565/1‑75 (MQ=255)
                                                                                                                           gAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGAta                               <  2:495775/107‑1 (MQ=255)
                                                                                                                           gAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGAta                               >  1:495775/1‑107 (MQ=255)
                                                                                                                                |                                                                                                                                  
ACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTG  >  NC_000913/2445125‑2445383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: