Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,736,687 T→C V181A (GTG→GCG)  bamD → BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,736,6870TC100.0% 162.6 / NA 44V181A (GTG→GCG) bamDBamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/25);  total (19/25)

TCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGC  >  NC_000913/2736553‑2736821
                                                                                                                                      |                                                                                                                                      
tcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    <  2:1061446/139‑1 (MQ=255)
tcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    <  2:978238/139‑1 (MQ=255)
  cTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGa                                                                                                                                  <  1:630079/139‑1 (MQ=255)
   tCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAg                                                                                                                                 <  1:304736/139‑1 (MQ=255)
   tCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAg                                                                                                                                 <  1:791999/139‑1 (MQ=255)
                    cGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTgg                                                                                                                >  2:167195/1‑139 (MQ=255)
                              cTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGa                                                                                                                                  <  2:917926/111‑1 (MQ=255)
                              cTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGa                                                                                                                                  >  1:917926/1‑111 (MQ=255)
                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    <  1:1221748/108‑1 (MQ=255)
                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    >  2:1221748/1‑108 (MQ=255)
                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTg                                                                                                     <  1:124372/139‑1 (MQ=255)
                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTg                                                                                                     <  2:723306/139‑1 (MQ=255)
                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTg                                                                                                     <  2:1086120/139‑1 (MQ=255)
                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTg                                                                                                     <  1:167195/139‑1 (MQ=255)
                                           gTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAAcc                                                                                         >  1:903789/1‑139 (MQ=255)
                                            tGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACcg                                                                                        >  1:511655/1‑139 (MQ=255)
                                               gcgGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATACGAATACTCCGCGGcc                                                                                                                                    >  1:939568/1‑92 (MQ=255)
                                               gcgGCTATCCGAACAGTCAGTACACCACAGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    <  2:939568/92‑1 (MQ=255)
                                                   cTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGaa                                                                                 >  1:254777/1‑139 (MQ=255)
                                                               tcagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTgcgc                                                                     <  2:903789/139‑1 (MQ=255)
                                                                cagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTa                                                                                                                               <  1:735779/80‑1 (MQ=255)
                                                                cagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTa                                                                                                                               >  2:735779/1‑80 (MQ=255)
                                                                 agtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    <  1:55699/74‑1 (MQ=255)
                                                                 agtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    >  2:55699/1‑74 (MQ=255)
                                                                   tACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACt                                                                 <  2:47680/139‑1 (MQ=255)
                                                                        caccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCgg                                                                                                                                      <  2:300176/65‑1 (MQ=255)
                                                                        caccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCgg                                                                                                                                      >  1:300176/1‑65 (MQ=255)
                                                                        caccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCg                                                            <  2:681322/139‑1 (MQ=255)
                                                                        caccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCg                                                            <  2:254777/139‑1 (MQ=255)
                                                                             aTGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGa                                                                                                                                  <  2:1141357/64‑1 (MQ=255)
                                                                             aTGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGa                                                                                                                                  >  1:1141357/1‑64 (MQ=255)
                                                                             aTGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGt                                                                                                                  >  2:1166756/1‑80 (MQ=255)
                                                                             aTGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGt                                                                                                                  <  1:1166756/80‑1 (MQ=255)
                                                                                           cTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAATGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTg                                         >  1:857814/1‑139 (MQ=255)
                                                                                            tGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGa                                        >  1:610927/1‑139 (MQ=255)
                                                                                                      gAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCg                              <  2:511655/139‑1 (MQ=255)
                                                                                                         aGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAcc                                                      <  2:18385/112‑1 (MQ=255)
                                                                                                         aGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAcc                                                      >  1:18385/1‑112 (MQ=255)
                                                                                                             cGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGa                                                          <  2:876231/104‑1 (MQ=255)
                                                                                                             cGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGa                                                          >  1:876231/1‑104 (MQ=255)
                                                                                                                     gAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCa               <  2:610927/139‑1 (MQ=255)
                                                                                                                                 cTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagatg   >  1:658231/1‑139 (MQ=255)
                                                                                                                                  tCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagatgc  >  2:25112/1‑139 (MQ=255)
                                                                                                                                   ccGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCAt              >  1:1280695/1‑126 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGC  >  NC_000913/2736553‑2736821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑