| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,845,226 | G→A | P416S (CCA→TCA) | hycE ← | hydrogenase 3, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,845,226 | 0 | G | A | 93.8% | 96.2 / ‑1.0 | 32 | P416S (CCA→TCA) | hycE | hydrogenase 3, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (19/11); total (20/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
CTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGC > NC_000913/2845091‑2845359 | cTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGacag < 2:733359/139‑1 (MQ=255) ccaccaTCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGAcagcag > 2:408038/1‑139 (MQ=255) tCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATa > 1:734938/1‑139 (MQ=255) aGTGGGCCACCCGGCAGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGc > 2:119880/1‑139 (MQ=255) gCCACCCGGCAGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACa < 1:598289/139‑1 (MQ=255) ccACCCGGCAGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACaa > 1:169490/1‑139 (MQ=255) ccGGCAGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACgg > 2:655070/1‑139 (MQ=255) aGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGAt < 2:169490/139‑1 (MQ=255) aGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGAt < 1:488681/139‑1 (MQ=255) ccGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTAt > 2:1309547/1‑139 (MQ=255) tAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCAc > 1:1150243/1‑139 (MQ=255) aGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACg > 1:1137382/1‑139 (MQ=255) tGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAGCGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGAcc > 1:571240/1‑139 (MQ=255) cgcgGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACaaa > 1:784231/1‑99 (MQ=255) cgcgGTAGAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACaaa < 2:784231/99‑1 (MQ=255) cGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGcc > 2:634269/1‑139 (MQ=255) gTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACa < 1:534887/82‑1 (MQ=255) gTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACa > 2:534887/1‑82 (MQ=255) gTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCg > 1:589856/1‑139 (MQ=255) gTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCg > 1:101906/1‑139 (MQ=255) cACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTg < 1:408038/139‑1 (MQ=255) tttCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTGAAg > 1:916263/1‑139 (MQ=255) aCGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGa > 2:1053897/1‑100 (MQ=255) aCGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGa < 1:1053897/100‑1 (MQ=255) cGCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTc < 2:1137382/139‑1 (MQ=255) gCAGCCCTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTcc > 1:409953/1‑139 (MQ=255) cTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAgacga < 2:1019257/139‑1 (MQ=255) cTGCTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAgacga < 1:655070/139‑1 (MQ=255) cTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGgcgc > 1:489836/1‑56 (MQ=255) cTCGCTGTGGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGgcgc < 2:489836/56‑1 (MQ=255) tgtgGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGc > 1:419750/1‑90 (MQ=255) tgtgGACTTCCATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGc < 2:419750/90‑1 (MQ=255) cATTGACAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTgcgc > 1:311131/1‑139 (MQ=255) | CTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGTCGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGC > NC_000913/2845091‑2845359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |