Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,035,437	T→C	K282R (AAG→AGG)	prfB ←	peptide chain release factor RF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,035,437	0	T	C	100.0%	199.4 / NA	54	K282R (AAG→AGG)	prfB	peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/29);  total (25/29)

TAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGAT  >  NC_000913/3035305‑3035569                                                                                                                                     |                                                                                                                                     tAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCt                                                                                                                                >  1:1200146/1‑139 (MQ=255) tAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCt                                                                                                                                >  2:256852/1‑139 (MQ=255)    gAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTggg                                                                                                                             >  1:344760/1‑139 (MQ=255)        gAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACg                                                                                                                         >  1:915580/1‑139 (MQ=255)          aTCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGt                                                                                                                       >  1:259954/1‑139 (MQ=255)              ggCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGtt                                                                                                                   <  2:259954/139‑1 (MQ=255)                cTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCt                                                                                                                 >  2:908484/1‑139 (MQ=255)                    cccAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCa                                                                                                             <  1:440806/139‑1 (MQ=255)                            aTGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCa                                                                                                     <  1:304577/139‑1 (MQ=255)                             tGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCAc                                                                                                    >  2:542371/1‑139 (MQ=255)                             tGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCAc                                                                                                    >  1:427349/1‑139 (MQ=255)                                  gATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATcc                                                                                               <  2:915580/139‑1 (MQ=255)                                    tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCg                                                                                             <  1:256852/139‑1 (MQ=255)                                     ttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTTCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGGACGGTCGTTCTGGCACTGGGGGACGATCCCgg                                                                                            <  1:904478/139‑1 (MQ=255)                                      tGTTATCTTCCATCGCCTGTTTCTCGTCATTTTTCTTCTGCATCTCCAATTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGt                                                                                           <  1:1155004/139‑1 (MQ=255)                                         tATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCgg                                                                                        <  2:25093/139‑1 (MQ=255)                                         tATCTTCCATCGCCTGTCTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTCGCACGGGGTCACGATCCAGGTCgg                                                                                        >  1:1175457/1‑139 (MQ=255)                                                cATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                 <  1:748337/139‑1 (MQ=255)                                                cATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                 <  1:1066385/139‑1 (MQ=255)                                                     ccTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGt                                                                                           >  2:739111/1‑124 (MQ=255)                                                     ccTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGt                                                                                           <  1:739111/124‑1 (MQ=255)                                                                      ttcttcTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCgg                                                           >  1:1213798/1‑139 (MQ=255)                                                                         ttctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTAc                                                        <  2:1200146/139‑1 (MQ=255)                                                                           ctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACgg                                                      <  2:344760/139‑1 (MQ=255)                                                                             gCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGtt                                                    <  2:427349/139‑1 (MQ=255)                                                                                            aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCggg                                                                                       >  1:316951/1‑89 (MQ=255)                                                                                            aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCggg                                                                                       <  2:316951/89‑1 (MQ=255)                                                                                            aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcc                                     >  1:556789/1‑139 (MQ=255)                                                                                            aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcc                                     <  2:1175457/139‑1 (MQ=255)                                                                                                  tCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGcc                               >  2:916131/1‑139 (MQ=255)                                                                                                   cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACt                                                                                                           >  2:167612/1‑62 (MQ=255)                                                                                                   cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACt                                                                                                           <  1:167612/62‑1 (MQ=255)                                                                                                   cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCg                              >  1:580143/1‑139 (MQ=255)                                                                                                   cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCg                              >  2:486839/1‑139 (MQ=255)                                                                                                   cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCg                              >  1:305726/1‑139 (MQ=255)                                                                                                    gCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCgg                             >  1:1206293/1‑139 (MQ=255)                                                                                                       ttCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACg                          <  2:556789/139‑1 (MQ=255)                                                                                                         cATCTGCTTCATGGCCTGATTTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGAATGTGGGTAATACGCACCGAAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTg                        <  2:580143/139‑1 (MQ=255)                                                                                                         cATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTg                        <  1:106866/139‑1 (MQ=255)                                                                                                               cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcc                                     <  1:216733/120‑1 (MQ=255)                                                                                                               cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcc                                     >  2:216733/1‑120 (MQ=255)                                                                                                                ttCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaaa                 >  1:469603/1‑139 (MQ=255)                                                                                                                ttCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaaa                 <  2:729398/139‑1 (MQ=255)                                                                                                                     ggCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCa            >  2:1019484/1‑139 (MQ=255)                                                                                                                     ggCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCa            <  2:690030/139‑1 (MQ=255)                                                                                                                         tGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAt                                                                                     >  1:637817/1‑62 (MQ=255)                                                                                                                         tGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAt                                                                                     <  2:637817/62‑1 (MQ=255)                                                                                                                          gATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCa                                                                                                             >  1:1148856/1‑37 (MQ=255)                                                                                                                          gATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCa                                                                                                             <  2:1148856/37‑1 (MQ=255)                                                                                                                          gATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAc                           <  2:33730/119‑1 (MQ=255)                                                                                                                          gATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAc                           >  1:33730/1‑119 (MQ=255)                                                                                                                          gATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATgcg       <  1:916131/139‑1 (MQ=255)                                                                                                                             cTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAg    <  1:1099520/139‑1 (MQ=255)                                                                                                                               ttGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTACTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGAt  <  1:873735/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     TAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGAT  >  NC_000913/3035305‑3035569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑