Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,418,135 T→C *1035Q (TAA→CAA)  acrF → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,418,1350TC100.0% 385.4 / NA 102*1035Q (TAA→CAA) acrFmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/48);  total (54/48)

Only 100 of 102 total aligned reads displayed.

CGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCCCGCTCCCTTCACTATTTTTA  >  NC_000913/3418008‑3418264
                                                                                                                               |                                                                                                                                 
cGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAg                                                                                                                            <  2:1187462/135‑1 (MQ=255)
cGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAg                                                                                                                            >  1:1187462/1‑135 (MQ=255)
cGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAAc                                                                                                                        >  2:948192/1‑139 (MQ=255)
   tGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGa                                                                                                                           <  2:885109/133‑1 (MQ=255)
   tGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGa                                                                                                                           >  1:885109/1‑133 (MQ=255)
   tGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATa                                                                                                                     >  1:349276/1‑139 (MQ=255)
     ccGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATaaa                                                                                                                   >  2:9032/1‑139 (MQ=255)
       ggCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAgg                                                                                                                 >  1:49092/1‑139 (MQ=255)
       ggCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAgg                                                                                                                 >  2:1127383/1‑139 (MQ=255)
       ggCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAgg                                                                                                                 >  1:392691/1‑139 (MQ=255)
                 cgcAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       >  1:1153432/1‑139 (MQ=255)
                 cgcAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       <  2:391539/139‑1 (MQ=255)
                 cgcAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTGGTGGTGATCCGCCGTTGCGTTAAAGGACAAATCAGAAACACAAAGGCGCTTTCggg                                                                                                       <  1:916883/139‑1 (MQ=255)
                    aGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGc                                                                                                    <  1:509027/139‑1 (MQ=255)
                      aaCGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCt                                                                                                  <  1:632801/139‑1 (MQ=255)
                      aaCGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCt                                                                                                  >  2:925202/1‑139 (MQ=255)
                      aaCGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCt                                                                                                  >  2:632801/1‑139 (MQ=255)
                              gggTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTattatt                                                                                          <  2:670717/139‑1 (MQ=255)
                                  aTCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTACGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCa                                                                                      >  2:1111012/1‑139 (MQ=255)
                                    cGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       <  2:139779/120‑1 (MQ=255)
                                    cGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       >  1:139779/1‑120 (MQ=255)
                                       ggTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       >  2:368949/1‑117 (MQ=255)
                                       ggTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       <  1:368949/117‑1 (MQ=255)
                                       ggTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGaa                                                                                 >  2:1006060/1‑139 (MQ=255)
                                            tGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       <  1:175195/112‑1 (MQ=255)
                                            tGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       >  2:175195/1‑112 (MQ=255)
                                             gggAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCAt                                                                           <  2:392691/139‑1 (MQ=255)
                                             gggAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCAt                                                                           <  1:174515/139‑1 (MQ=255)
                                                 ggaATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCt                                                                                                  >  1:737185/1‑112 (MQ=255)
                                                 ggaATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCt                                                                                                  <  2:737185/112‑1 (MQ=255)
                                                     tGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATta                                                                   >  2:283377/1‑139 (MQ=255)
                                                     tGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATta                                                                   >  2:51727/1‑139 (MQ=255)
                                                      ggTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATtaa                                                                  <  2:1153432/139‑1 (MQ=255)
                                                        tctcTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATtaata                                                                <  2:161604/139‑1 (MQ=255)
                                                        tctcTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATtaata                                                                <  1:815517/139‑1 (MQ=255)
                                                                 cGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       >  2:854297/1‑91 (MQ=255)
                                                                 cGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       <  1:854297/91‑1 (MQ=255)
                                                                    tGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       >  2:249590/1‑88 (MQ=255)
                                                                    tGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       <  1:249590/88‑1 (MQ=255)
                                                                    tGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGc                                                                                                    >  1:298724/1‑91 (MQ=255)
                                                                    tGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGc                                                                                                    <  2:298724/91‑1 (MQ=255)
                                                                      cTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGgcgc                                                                                                              <  1:1099792/79‑1 (MQ=255)
                                                                      cTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGgcgc                                                                                                              >  2:1099792/1‑79 (MQ=255)
                                                                      cTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       <  2:112053/86‑1 (MQ=255)
                                                                      cTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       >  1:112053/1‑86 (MQ=255)
                                                                      cTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATtaata                                                                <  2:1131202/125‑1 (MQ=255)
                                                                      cTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATtaata                                                                >  1:1131202/1‑125 (MQ=255)
                                                                       tGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCt                                                 <  1:283377/139‑1 (MQ=255)
                                                                       tGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCt                                                 <  1:925202/139‑1 (MQ=255)
                                                                       tGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCt                                                 <  1:1127383/139‑1 (MQ=255)
                                                                         gCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCa                                               <  1:51727/139‑1 (MQ=255)
                                                                             tcttctTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCGAAACATAAAGGCGCTTTCggg                                                                                                       <  2:796173/78‑1 (MQ=255)
                                                                             tcttctTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCGAAACATAAAGGCGCTTTCggg                                                                                                       >  1:796173/1‑78 (MQ=255)
                                                                                 cttcGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTaa                                       <  1:537764/139‑1 (MQ=255)
                                                                                    cGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAg                                                                                                                  <  1:18196/61‑1 (MQ=255)
                                                                                    cGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAg                                                                                                                  >  2:18196/1‑61 (MQ=255)
                                                                                    cGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAg                                    <  1:719413/139‑1 (MQ=255)
                                                                                       aCCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCtt                                 >  2:507536/1‑139 (MQ=255)
                                                                                         cGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATaaa                                                                        >  2:1225357/1‑98 (MQ=255)
                                                                                         cGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATaaa                                                                        <  1:1225357/98‑1 (MQ=255)
                                                                                           gtgtTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       <  1:192244/65‑1 (MQ=255)
                                                                                           gtgtTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       >  2:192244/1‑65 (MQ=255)
                                                                                           gtgtTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCgg                                                                                                        >  2:323262/1‑64 (MQ=255)
                                                                                           gtgtTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCgg                                                                                                        <  1:323262/64‑1 (MQ=255)
                                                                                           gtgtTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGa                                                             <  2:806390/107‑1 (MQ=255)
                                                                                           gtgtTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGa                                                             >  1:806390/1‑107 (MQ=255)
                                                                                           gtgtTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCta                             >  1:1011756/1‑139 (MQ=255)
                                                                                           gtgtTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAACTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCta                             >  1:830629/1‑139 (MQ=255)
                                                                                            tgtTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTtattat                                                                                           <  1:450106/76‑1 (MQ=255)
                                                                                            tgtTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTtattat                                                                                           >  2:450106/1‑76 (MQ=255)
                                                                                              ttCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCt                                  >  2:706419/1‑131 (MQ=255)
                                                                                              ttCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCt                                  <  1:706419/131‑1 (MQ=255)
                                                                                              ttCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAg                          >  2:648858/1‑139 (MQ=255)
                                                                                              ttCTTTGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAg                          >  2:1122241/1‑139 (MQ=255)
                                                                                                 tttGTGGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCtt                                 >  1:962482/1‑129 (MQ=255)
                                                                                                   tgtgGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCt                                                                                                  >  1:776771/1‑62 (MQ=255)
                                                                                                   tgtgGTGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCt                                                                                                  <  2:776771/62‑1 (MQ=255)
                                                                                                   tgtgGTGATACGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCCTTCGGGTGCCTTTCTTATTTCCCGTGAAACCCATAAAAATTAATAAGATCTTCTTCTGCTAACTCTTTACAAGATTTCTATAGTTcct                     >  1:599320/1‑138 (MQ=255)
                                                                                                   tgtgGCGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAg                                                                                                                  >  2:799051/1‑46 (MQ=255)
                                                                                                   tgtgGCGATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAg                                                                                                                  <  1:799051/46‑1 (MQ=255)
                                                                                                      ggtgATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTaa                                       >  2:431556/1‑118 (MQ=255)
                                                                                                      ggtgATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTaa                                       <  1:431556/118‑1 (MQ=255)
                                                                                                      ggtgATCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCCCGCt                  >  2:1122272/1‑139 (MQ=255)
                                                                                                          aTCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTc                       >  1:1202954/1‑130 (MQ=255)
                                                                                                          aTCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTc                       <  2:1202954/130‑1 (MQ=255)
                                                                                                          aTCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCCCg                    >  2:465924/1‑133 (MQ=255)
                                                                                                          aTCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCCCg                    <  1:465924/133‑1 (MQ=255)
                                                                                                          aTCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCCCGCTCCCt              >  1:1306232/1‑139 (MQ=255)
                                                                                                           tCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTc                                                                                        <  2:449280/64‑1 (MQ=255)
                                                                                                           tCCGCCGTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTc                                                                                        >  1:449280/1‑64 (MQ=255)
                                                                                                             cgccgTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCCCGCTCCCTTCa           >  1:803431/1‑139 (MQ=255)
                                                                                                               ccgTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCt                                                 >  2:419357/1‑99 (MQ=255)
                                                                                                               ccgTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCt                                                 <  1:419357/99‑1 (MQ=255)
                                                                                                               ccgTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCCCGCTCCCTTCACt         >  1:219864/1‑139 (MQ=255)
                                                                                                               ccgTTGCTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCCCGCTCCCTTCACt         >  2:1125017/1‑139 (MQ=255)
                                                                                                                     cTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATtaa                                                                  <  1:119526/76‑1 (MQ=255)
                                                                                                                     cTTTAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATtaa                                                                  >  2:119526/1‑76 (MQ=255)
                                                                                                                      tttAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       >  1:133151/1‑38 (MQ=255)
                                                                                                                      tttAAAGGACAAATCAGAAACATAAAGGCGCTTTCggg                                                                                                       <  2:133151/38‑1 (MQ=255)
                                                                                                                      tttAAAGGACAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCCCGCTCCCTTCACTATTTTTa  <  2:238861/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                 
CGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCCCGCTCCCTTCACTATTTTTA  >  NC_000913/3418008‑3418264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: