Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 884,506 G→A L278L (CTG→CTA)  mdfA → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913884,5060GA100.0% 229.0 / NA 65L278L (CTG→CTA) mdfAmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/33);  total (32/33)

GCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTA  >  NC_000913/884371‑884636
                                                                                                                                       |                                                                                                                                  
gCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAAcc                                                                                                                                 <  1:554860/139‑1 (MQ=255)
 ccGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCt                                                                                                                                >  2:1031164/1‑139 (MQ=255)
     tGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTcgcg                                                                                                                            <  1:1031164/139‑1 (MQ=255)
                    cccAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTc                                                                                                             <  1:735098/139‑1 (MQ=255)
                    cccAGTCGCCGACTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTc                                                                                                             >  1:486897/1‑139 (MQ=255)
                                          tACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTgg                                                                                       >  2:1130461/1‑139 (MQ=255)
                                          tACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGCTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTgg                                                                                       >  1:554169/1‑139 (MQ=255)
                                           aCCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGc                                                                                      >  1:781496/1‑139 (MQ=255)
                                             cGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCg                                                                                    >  1:243162/1‑139 (MQ=255)
                                              ggCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGa                                                                                   >  1:61570/1‑139 (MQ=255)
                                               gCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCg                                                                                                            >  1:1154562/1‑113 (MQ=255)
                                               gCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCg                                                                                                            <  2:1154562/113‑1 (MQ=255)
                                               gCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATg                                                                                                <  1:1269848/125‑1 (MQ=255)
                                               gCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATg                                                                                                >  2:1269848/1‑125 (MQ=255)
                                                cGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGc                                                                                                           >  1:1089897/1‑113 (MQ=255)
                                                cGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGc                                                                                                           <  2:1089897/113‑1 (MQ=255)
                                                    cAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGa                                                                             <  1:939962/139‑1 (MQ=255)
                                                    cAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGa                                                                             >  2:939962/1‑139 (MQ=255)
                                                     aGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGAt                                                                            <  1:906325/139‑1 (MQ=255)
                                                     aGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGAt                                                                            <  1:1051839/139‑1 (MQ=255)
                                                      gTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGAtt                                                                           <  2:243162/139‑1 (MQ=255)
                                                              gcTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTAtt                                                                   >  2:206854/1‑139 (MQ=255)
                                                               cTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGcgccg                                                                                                                         <  2:764571/84‑1 (MQ=255)
                                                               cTATGAATATGACTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGcgccg                                                                                                                         >  1:764571/1‑84 (MQ=255)
                                                                 aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGcgccg                                                                                                                         >  1:556132/1‑82 (MQ=255)
                                                                 aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGcgccg                                                                                                                         <  2:556132/82‑1 (MQ=255)
                                                                 aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCg                                                                                                                    >  1:53216/1‑87 (MQ=255)
                                                                 aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCg                                                                                                                    <  2:53216/87‑1 (MQ=255)
                                                                 aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTgg                                                                                       >  1:192704/1‑116 (MQ=255)
                                                                 aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTgg                                                                                       <  2:192704/116‑1 (MQ=255)
                                                                 aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGt                                                                >  2:596623/1‑139 (MQ=255)
                                                                 aTGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGt                                                                <  1:267187/139‑1 (MQ=255)
                                                                         ggCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCg                                                                                                            >  2:648813/1‑87 (MQ=255)
                                                                         ggCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCg                                                                                                            <  1:648813/87‑1 (MQ=255)
                                                                         ggCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGctgctg                                                        >  1:1043165/1‑139 (MQ=255)
                                                                           cTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGAt                                                                            >  2:1148820/1‑117 (MQ=255)
                                                                           cTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGAt                                                                            <  1:1148820/117‑1 (MQ=255)
                                                                            ttGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCgg                                                     <  2:61570/139‑1 (MQ=255)
                                                                             tgctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGc                                                    <  1:320982/139‑1 (MQ=255)
                                                                              gctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCg                                                                                                            >  2:1291261/1‑82 (MQ=255)
                                                                              gctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCg                                                                                                            <  1:1291261/82‑1 (MQ=255)
                                                                              gctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCa                                                   <  1:1130461/139‑1 (MQ=255)
                                                                              gctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCa                                                   >  2:831482/1‑139 (MQ=255)
                                                                              gctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCa                                                   >  2:1103138/1‑139 (MQ=255)
                                                                              gctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCa                                                   >  1:620800/1‑139 (MQ=255)
                                                                              gctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCa                                                   >  1:571293/1‑139 (MQ=255)
                                                                              gctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCa                                                   <  2:571293/139‑1 (MQ=255)
                                                                              gctgcAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCa                                                   <  2:486897/139‑1 (MQ=255)
                                                                                             tttCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCg                                    <  2:620800/139‑1 (MQ=255)
                                                                                               tCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCa                                  <  2:1043165/139‑1 (MQ=255)
                                                                                                cGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCAc                                 >  2:970172/1‑139 (MQ=255)
                                                                                                  gggCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCAcgc                               <  2:781496/139‑1 (MQ=255)
                                                                                                   ggCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCAcgcg                              <  1:446852/139‑1 (MQ=255)
                                                                                                      gTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGt                                                                                                               <  2:69705/55‑1 (MQ=255)
                                                                                                      gTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGt                                                                                                               >  1:69705/1‑55 (MQ=255)
                                                                                                             gCGGGTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGACAACGGTTATCTCATCGCACGCGTATTTATGGa                    <  1:1220717/139‑1 (MQ=255)
                                                                                                               gggTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCg                                    <  1:237671/121‑1 (MQ=255)
                                                                                                               gggTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCg                                    >  2:237671/1‑121 (MQ=255)
                                                                                                                 gTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGc                                                                                                           <  2:285341/48‑1 (MQ=255)
                                                                                                                 gTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGc                                                                                                           >  1:285341/1‑48 (MQ=255)
                                                                                                                 gTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTAt                                           <  2:583188/112‑1 (MQ=255)
                                                                                                                 gTAACTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTAt                                           >  1:583188/1‑112 (MQ=255)
                                                                                                                    aCTTGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGc             <  1:970172/139‑1 (MQ=255)
                                                                                                                      ttGCTGTTAGCGCGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCg           >  1:729469/1‑139 (MQ=255)
                                                                                                                               gcgcGTCTAACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTa  >  1:179888/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                  
GCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTA  >  NC_000913/884371‑884636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑