Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,808	A→G	intergenic (‑40/+26)	pyrE ← / ← rph	orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,808	0	A	G	100.0%	195.1 / NA	54	intergenic (‑40/+26)	pyrE/rph	orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/29);  total (25/29)

AGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGC  >  NC_000913/3815672‑3815929                                                                                                                                         |                                                                                                                          aGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAggg                                                                                                                         <  2:989667/139‑1 (MQ=255)  ggggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGc                                                                                                                        <  2:279949/139‑1 (MQ=255)             gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGt                                                                                                             <  1:790231/139‑1 (MQ=255)                         gCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAAt                                                                                                 >  2:118660/1‑139 (MQ=255)                             aaaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGt                                                                                             >  2:1119478/1‑139 (MQ=255)                               aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGttt                                                                                           >  1:1265506/1‑139 (MQ=255)                                 tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                         <  1:816206/139‑1 (MQ=255)                                    ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGc                                                                                                                        <  2:769028/105‑1 (MQ=255)                                    ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGc                                                                                                                        >  1:769028/1‑105 (MQ=255)                                    ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAg                                                                                      >  1:15153/1‑139 (MQ=255)                                      aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                    <  2:1185904/139‑1 (MQ=255)                                      aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                    <  2:15153/139‑1 (MQ=255)                                        aCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                  <  1:1287876/139‑1 (MQ=255)                                           ttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGc                                                                                                                        <  1:719433/98‑1 (MQ=255)                                           ttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGc                                                                                                                        >  2:719433/1‑98 (MQ=255)                                             aaCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCtt                                                                             >  2:1042186/1‑139 (MQ=255)                                                   tgcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                       <  1:118660/139‑1 (MQ=255)                                                              cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGt                                                                                                             <  1:1107712/90‑1 (MQ=255)                                                              cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGt                                                                                                             >  2:1107712/1‑90 (MQ=255)                                                              cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATgg                                                            <  1:1119478/139‑1 (MQ=255)                                                              cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATgg                                                            >  1:595206/1‑139 (MQ=255)                                                               gcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGga                                                           >  2:840911/1‑139 (MQ=255)                                                                  aaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                    >  2:303483/1‑111 (MQ=255)                                                                  aaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                    <  1:303483/111‑1 (MQ=255)                                                                           aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCt                                               <  2:384993/139‑1 (MQ=255)                                                                           aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCt                                               <  2:595206/139‑1 (MQ=255)                                                                            aCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTc                                              <  1:382540/139‑1 (MQ=255)                                                                              tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                       <  2:20930/112‑1 (MQ=255)                                                                              tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                       >  1:20930/1‑112 (MQ=255)                                                                              tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                                            >  1:214281/1‑139 (MQ=255)                                                                                gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGc                                          <  1:920279/139‑1 (MQ=255)                                                                                 cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCggg                                           <  2:757607/137‑1 (MQ=255)                                                                                 cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCggg                                           >  1:757607/1‑137 (MQ=255)                                                                                 cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGc                                          >  2:275223/1‑138 (MQ=255)                                                                                 cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGc                                          <  1:275223/138‑1 (MQ=255)                                                                                   cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                  >  1:1058506/1‑96 (MQ=255)                                                                                   cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                  <  2:1058506/96‑1 (MQ=255)                                                                                          ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaac                                <  2:737580/139‑1 (MQ=255)                                                                                             ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACTAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAg                             <  1:840911/139‑1 (MQ=255)                                                                                                aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCg                                                                                                           >  2:967011/1‑58 (MQ=255)                                                                                                aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCg                                                                                                           <  1:967011/58‑1 (MQ=255)                                                                                                aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                          >  2:1193019/1‑139 (MQ=255)                                                                                                aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                          >  1:1282707/1‑139 (MQ=255)                                                                                                aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                          >  1:1299679/1‑139 (MQ=255)                                                                                                aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGA‑TCGATTCCCCTCGGGCCag                                       >  1:1117209/1‑125 (MQ=255)                                                                                                aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGA‑TCGATTCCCCTCGGGCCag                                       <  2:1117209/125‑1 (MQ=255)                                                                                                  gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg                        <  1:169458/139‑1 (MQ=255)                                                                                                       cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGct                   <  2:985113/139‑1 (MQ=255)                                                                                                            ctcATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                                    >  2:242641/1‑85 (MQ=255)                                                                                                            ctcATCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                                    <  1:242641/85‑1 (MQ=255)                                                                                                               aTCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                                    >  2:438718/1‑82 (MQ=255)                                                                                                               aTCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                                    <  1:438718/82‑1 (MQ=255)                                                                                                               aTCTTACTTTTCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg           >  1:936026/1‑139 (MQ=255)                                                                                                                      ttttCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACg    >  2:919188/1‑139 (MQ=255)                                                                                                                        ttCTACAGACAAAAAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc  <  2:1299679/139‑1 (MQ=255)                                                                                                                                  aaaaaaagGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                     <  2:1055181/110‑1 (MQ=255)                                                                                                                                  aaaaaaagGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                     >  1:1055181/1‑110 (MQ=255)                                                                                                                                         |                                                                                                                          AGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGC  >  NC_000913/3815672‑3815929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑