| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,022,508 | A→G | V459A (GTG→GCG) | yccS ← | putative transporter, FUSC superfamily inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,022,508 | 0 | A | G | 97.1% | 251.8 / ‑2.1 | 71 | V459A (GTG→GCG) | yccS | putative transporter, FUSC superfamily inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (29/39); total (31/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01 | |||||||||||
TTCACCCAGTAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGA > NC_000913/1022372‑1022642 | ttCACCCAGTAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGcgca > 1:821746/1‑139 (MQ=255) ccAGTAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACca < 1:360026/139‑1 (MQ=255) gTAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACcacag < 2:478222/139‑1 (MQ=255) caGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAgg < 1:687009/132‑1 (MQ=255) caGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAgg > 2:687009/1‑132 (MQ=255) gTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAAt > 1:1231974/1‑139 (MQ=255) cACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGcc > 1:195682/1‑132 (MQ=255) cACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGcc < 2:195682/132‑1 (MQ=255) cACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCg < 1:973148/139‑1 (MQ=255) cACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCg < 1:495644/139‑1 (MQ=255) cACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCg < 1:1101939/139‑1 (MQ=255) ccAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGAt < 1:300489/139‑1 (MQ=255) aaaGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACcacagca < 2:425603/117‑1 (MQ=255) aaaGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACcacagca > 1:425603/1‑117 (MQ=255) aaaGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGAtacc > 1:909862/1‑139 (MQ=255) aGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGAtaccta > 1:603613/1‑139 (MQ=255) gtgtGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGAtacctac < 1:737309/139‑1 (MQ=255) gtgtGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGAtacctac < 2:512264/139‑1 (MQ=255) atgaACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCg < 2:577660/139‑1 (MQ=255) atgaACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCg > 1:1032594/1‑139 (MQ=255) tgaACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGt < 1:435147/139‑1 (MQ=255) aTCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCaa < 1:1216924/139‑1 (MQ=255) aTCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCaa < 1:1144368/139‑1 (MQ=255) aTCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGAACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGACAATGGCGATACCTACCAGCGTACCaa < 2:796994/139‑1 (MQ=255) cGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAg < 2:1071883/129‑1 (MQ=255) cGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAg > 1:1071883/1‑129 (MQ=255) ttGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGa > 1:998546/1‑104 (MQ=255) ttGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGa < 2:998546/104‑1 (MQ=255) aTGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCtt > 1:1315755/1‑139 (MQ=255) gCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAAc > 1:135100/1‑84 (MQ=255) gCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAAc < 2:135100/84‑1 (MQ=255) cGTATTGCACGATACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCAAGTGATTGCCCAAACCACAGCACAGGAATGACAATGGCGATACCTACCAGCGTACCAATAATCCTTATCtt > 1:965713/1‑139 (MQ=255) gCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCg < 1:289452/139‑1 (MQ=255) gTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGAtacctacc > 1:1254889/1‑106 (MQ=255) gTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGAtacctacc < 2:1254889/106‑1 (MQ=255) tACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGcc < 2:2283/88‑1 (MQ=255) tACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGcc > 1:2283/1‑88 (MQ=255) tACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGgcg > 2:321976/1‑139 (MQ=255) cGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGcgcg < 1:249095/139‑1 (MQ=255) cGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGcgcg < 1:146386/139‑1 (MQ=255) gAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTg < 2:540826/139‑1 (MQ=255) cAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGAGGCGtt > 1:411345/1‑139 (MQ=255) aaaaaaGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGcc > 2:565803/1‑78 (MQ=255) aaaaaaGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGcc < 1:565803/78‑1 (MQ=255) aaaaaaGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATgg < 1:1160927/83‑1 (MQ=255) aaaaaaGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATgg > 2:1160927/1‑83 (MQ=255) aaaaaaGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTta > 1:1062319/1‑139 (MQ=255) aaaaaaGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTta > 1:933270/1‑139 (MQ=255) agCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTtg > 1:1105960/1‑139 (MQ=255) cACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACca > 2:614093/1‑54 (MQ=255) cACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACca < 1:614093/54‑1 (MQ=255) gCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTtggctgg < 1:999864/139‑1 (MQ=255) cGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTg > 1:284511/1‑119 (MQ=255) cGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTg < 2:284511/119‑1 (MQ=255) cGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCa > 2:4073/1‑139 (MQ=255) gcagcaCCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACt > 1:50024/1‑139 (MQ=255) cagcaCCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACtt < 2:1105960/139‑1 (MQ=255) caCCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCg > 1:622968/1‑68 (MQ=255) caCCAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCg < 2:622968/68‑1 (MQ=255) ccAGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAg > 1:1232448/1‑139 (MQ=255) aGCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCa < 2:1231974/139‑1 (MQ=255) gCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCg < 2:436565/50‑1 (MQ=255) gCTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCg > 1:436565/1‑50 (MQ=255) cTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAg > 1:264864/1‑135 (MQ=255) cTGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAg < 2:264864/135‑1 (MQ=255) tGCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGa < 1:321976/139‑1 (MQ=255) gCCCTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGAt > 1:112091/1‑139 (MQ=255) ccTTCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATcc < 2:249402/139‑1 (MQ=255) ttCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCaa < 2:821746/139‑1 (MQ=255) tCCAGTGATGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAAt < 2:603613/139‑1 (MQ=255) tGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGa < 2:555018/139‑1 (MQ=255) tGGCGCAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGa > 1:206679/1‑139 (MQ=255) | TTCACCCAGTAAGTTAAAACACAGTAGCACCAAAAGTGTGATGAACATCGTTGCATGAGCGTATTGCACGTTACGGAAGGCAAAAAAGAGCACGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGA > NC_000913/1022372‑1022642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |