Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,366,113	A→G	S221P (TCC→CCC)	dcuA ←	C4‑dicarboxylate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,366,113	0	A	G	100.0%	256.5 / NA	69	S221P (TCC→CCC)	dcuA	C4‑dicarboxylate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/33);  total (36/33)

TGCGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGAAGGACATCACCAGAACCGCCAGCA  >  NC_000913/4365991‑4366247                                                                                                                           |                                                                                                                                       tGCGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAAt                                                                                                                            >  1:722390/1‑135 (MQ=255) tGCGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAAt                                                                                                                            <  2:722390/135‑1 (MQ=255)  gCGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCt                                                                                                                       >  2:507578/1‑139 (MQ=255)   cGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCtt                                                                                                                      >  1:1106455/1‑139 (MQ=255)   cGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCtt                                                                                                                      >  1:816389/1‑139 (MQ=255)   cGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCtt                                                                                                                      >  2:600171/1‑139 (MQ=255)   cGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCtt                                                                                                                      >  2:457408/1‑139 (MQ=255)         tggtgTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAAt                                                                                                                            <  1:265749/127‑1 (MQ=255)         tggtgTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAAt                                                                                                                            >  2:265749/1‑127 (MQ=255)            tgtTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACcgcg                                                                                                             <  2:829733/139‑1 (MQ=255)             gtTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCgcgc                                                                                                            >  1:474665/1‑139 (MQ=255)             gtTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCgcgc                                                                                                            >  2:995880/1‑139 (MQ=255)              ttCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCa                                                                                                           >  2:97925/1‑139 (MQ=255)                  tcaGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTc                                                                                                       >  2:1190343/1‑139 (MQ=255)                         tttttCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAgg                                                                                                <  2:474665/139‑1 (MQ=255)                           tttCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGcc                                                                                              <  1:383040/139‑1 (MQ=255)                           tttCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGcc                                                                                              <  1:651895/139‑1 (MQ=255)                                     aCCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAAt                                                                                                                            <  2:182311/99‑1 (MQ=255)                                     aCCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAAt                                                                                                                            >  1:182311/1‑99 (MQ=255)                                     aCCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGa                                                                                    >  1:969503/1‑139 (MQ=255)                                      cccATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGAc                                                                                   >  2:518412/1‑139 (MQ=255)                                         aTGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGc                                                                                               >  2:179554/1‑124 (MQ=255)                                         aTGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGc                                                                                               <  1:179554/124‑1 (MQ=255)                                           gCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTg                                                                              >  1:1132714/1‑139 (MQ=255)                                             ttGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCg                                                                            <  1:1028955/139‑1 (MQ=255)                                                     ttGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCa                                                                                                  <  2:834482/109‑1 (MQ=255)                                                     ttGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCa                                                                                                  >  1:834482/1‑109 (MQ=255)                                                     ttGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCg                                                                    >  1:1170374/1‑139 (MQ=255)                                                     ttGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCg                                                                    >  1:405213/1‑139 (MQ=255)                                                     ttGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCg                                                                    >  2:1172481/1‑139 (MQ=255)                                                      tgatgaTTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCgg                                                                   <  1:1124573/139‑1 (MQ=255)                                                           atTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCg                                                                    >  2:645864/1‑133 (MQ=255)                                                           atTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCg                                                                    <  1:645864/133‑1 (MQ=255)                                                               cATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGTACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCaga                                                          >  1:928699/1‑139 (MQ=255)                                                                  aGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATC‑GCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCa                                                                                      <  1:924409/107‑1 (MQ=255)                                                                  aGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATC‑GCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCa                                                                                      >  2:924409/1‑107 (MQ=255)                                                                    aTAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGtt                                                     >  1:507667/1‑139 (MQ=255)                                                                    aTAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGtt                                                     <  2:405213/139‑1 (MQ=255)                                                                       aCCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACcg                                                                                                               <  2:429322/78‑1 (MQ=255)                                                                       aCCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACcg                                                                                                               >  1:429322/1‑78 (MQ=255)                                                                       aCCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTCGCGATAAATCGGATCGTCAGAGAGCTTgg                                                  >  1:853450/1‑139 (MQ=255)                                                                         cACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAg                                                <  1:457408/139‑1 (MQ=255)                                                                         cACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAg                                                <  1:168530/139‑1 (MQ=255)                                                                           cGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAAt                                                                      <  1:685941/115‑1 (MQ=255)                                                                           cGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGa                                                                  <  1:706117/119‑1 (MQ=255)                                                                           cGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGa                                                                  >  2:706117/1‑119 (MQ=255)                                                                           cGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGtt                                              <  2:969503/139‑1 (MQ=255)                                                                           cGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGtt                                              <  1:1189675/139‑1 (MQ=255)                                                                           cGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATATGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAAt                                                                      >  2:685941/1‑115 (MQ=255)                                                                              cAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGc                                                                             <  2:307723/105‑1 (MQ=255)                                                                              cAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGc                                                                             >  1:307723/1‑105 (MQ=255)                                                                              cAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGaa                                           >  1:1094272/1‑139 (MQ=255)                                                                              cAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGaa                                           <  2:928699/139‑1 (MQ=255)                                                                              cAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGaa                                           <  1:995880/139‑1 (MQ=255)                                                                                     gCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCaga                                                          >  2:302336/1‑117 (MQ=255)                                                                                     gCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCaga                                                          <  1:302336/117‑1 (MQ=255)                                                                                     gCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATa                                    <  1:1114466/139‑1 (MQ=255)                                                                                       ccAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGt                                  <  1:97925/139‑1 (MQ=255)                                                                                           cagGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATa                                    <  1:989098/133‑1 (MQ=255)                                                                                           cagGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATa                                    >  2:989098/1‑133 (MQ=255)                                                                                           cagGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGAcc                              <  2:1132714/139‑1 (MQ=255)                                                                                           cagGAACAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGAcc                              >  2:347177/1‑139 (MQ=255)                                                                                                aCAGCCAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCaggaa                         >  1:535283/1‑139 (MQ=255)                                                                                                    ccAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATa                                    <  1:797799/124‑1 (MQ=255)                                                                                                    ccAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATa                                    >  2:797799/1‑124 (MQ=255)                                                                                                        acggacgTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTg                                             >  1:1192345/1‑111 (MQ=255)                                                                                                        acggacgTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTg                                             <  2:1192345/111‑1 (MQ=255)                                                                                                                     cACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGAAGGACATCACCAGAACCGCcag    <  2:595654/139‑1 (MQ=255)                                                                                                                       ccGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGAAGGACATCACCAGAACCGCcagca  <  1:1172481/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                       TGCGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGAAGGACATCACCAGAACCGCCAGCA  >  NC_000913/4365991‑4366247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑