Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,044,785	A→G	V340A (GTT→GCT)	gfcE ←	putative O‑antigen capsule outer membrane auxillary protein export channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,044,785	0	A	G	100.0%	179.0 / NA	49	V340A (GTT→GCT)	gfcE	putative O‑antigen capsule outer membrane auxillary protein export channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/25);  total (24/25)

GATTGAGTTAAATTTTAGTTGGGCCATGTTTGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAA  >  NC_000913/1044650‑1044909                                                                                                                                        |                                                                                                                             gATTGAGTTAAATTTTAGTTGGGCCATGTTTGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCaa                                                                                                                           <  2:682438/139‑1 (MQ=255)     gAGTTAAATTTTAGTTGGGCCATGTTTGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTc                                                                                                                       >  1:1240048/1‑139 (MQ=255)           aaTTTTAGTTGGGCCATGTTTGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAgg                                                                                                                 >  2:285429/1‑139 (MQ=255)                           tGTTTGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttcc                                                                                                 <  1:1248137/139‑1 (MQ=255)                              ttGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              <  2:1030858/139‑1 (MQ=255)                                     cACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTa                                                                                       >  2:1308815/1‑139 (MQ=255)                                      aCCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTAt                                                                                      >  2:1010449/1‑139 (MQ=255)                                       ccGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATc                                                                                     >  2:931058/1‑139 (MQ=255)                                       ccGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATc                                                                                     >  2:83503/1‑139 (MQ=255)                                           gAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTg                                                                                 <  1:831608/139‑1 (MQ=255)                                                ctctGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGAt                                                                            <  2:1308438/139‑1 (MQ=255)                                                 tctGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATg                                                                           >  1:738337/1‑139 (MQ=255)                                                  ctGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGc                                                                          <  1:1010449/139‑1 (MQ=255)                                                         gTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTc                                                                   >  2:763572/1‑139 (MQ=255)                                                            aTGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCa                                                                <  2:1240048/139‑1 (MQ=255)                                                             tGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              <  1:316944/108‑1 (MQ=255)                                                             tGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              >  2:316944/1‑108 (MQ=255)                                                                 ccccGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAg                                                           <  1:83503/139‑1 (MQ=255)                                                                   ccGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCt                                                         >  2:432634/1‑139 (MQ=255)                                                                       aaaTCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATa                                                     <  1:1308815/139‑1 (MQ=255)                                                                            gtcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAatat                                                >  1:814031/1‑139 (MQ=255)                                                                            gtcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAatat                                                >  2:1014595/1‑139 (MQ=255)                                                                            gtcgGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAatat                                                >  2:317695/1‑139 (MQ=255)                                                                                  aCCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGa                                          <  2:166941/139‑1 (MQ=255)                                                                                          agaAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCa                                  <  1:195966/139‑1 (MQ=255)                                                                                                cacaCGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGc                            <  2:814031/139‑1 (MQ=255)                                                                                                cacaCGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGc                            <  2:460765/139‑1 (MQ=255)                                                                                                    cGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              >  2:1140080/1‑69 (MQ=255)                                                                                                    cGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              <  1:1140080/69‑1 (MQ=255)                                                                                                     ggTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTg                                              <  2:637474/116‑1 (MQ=255)                                                                                                     ggTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTg                                              >  1:637474/1‑116 (MQ=255)                                                                                                      gTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              >  2:640199/1‑67 (MQ=255)                                                                                                      gTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              <  1:640199/67‑1 (MQ=255)                                                                                                      gTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGc                                                                          <  1:561176/87‑1 (MQ=255)                                                                                                      gTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGc                                                                          >  2:561176/1‑87 (MQ=255)                                                                                                          cAACGAGCCTGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCg                  <  2:1112768/139‑1 (MQ=255)                                                                                                          cAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTc                                                             >  1:727709/1‑96 (MQ=255)                                                                                                          cAACGAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTc                                                             <  2:727709/96‑1 (MQ=255)                                                                                                              gAGCCAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGc              <  2:313790/139‑1 (MQ=255)                                                                                                                 ccAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              >  2:368208/1‑56 (MQ=255)                                                                                                                 ccAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              <  2:497734/56‑1 (MQ=255)                                                                                                                 ccAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              >  1:497734/1‑56 (MQ=255)                                                                                                                 ccAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAAttccgtt                                                                                              <  1:368208/56‑1 (MQ=255)                                                                                                                 ccAGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACg           >  1:333069/1‑139 (MQ=255)                                                                                                                   aGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTc                                                             <  1:179765/87‑1 (MQ=255)                                                                                                                   aGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTc                                                             >  2:179765/1‑87 (MQ=255)                                                                                                                   aGCGGCGCGGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGaa         <  2:333069/139‑1 (MQ=255)                                                                                                                        cgcgGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACa    >  1:958501/1‑139 (MQ=255)                                                                                                                          cgGTAGTGACATAAGCAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACaaa  >  2:398380/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                             GATTGAGTTAAATTTTAGTTGGGCCATGTTTGAATCCACCGTGAAGTCTCTGTCAGGTCATGCACCCCGGAAATCGTCGGTACCAGCAGAGAAATCACACGGTTCCAACGAGCCAGCGGCGCGGTAGTGACATAAACAATGTCGTAAGGTTCAAGCTGGAATTCCGTTCCGAGTATCATTGCCGATGCGTCCTTCGCATTCAGCTGATAAATATTGGCGATTTTGCCATTCTGCTTGTTTTGCGTTGCACGAATAACAAA  >  NC_000913/1044650‑1044909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑