Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,967,135	(C)5→6	coding (303/390 nt)	cheY ←	chemotaxis regulator transmitting signal to flagellar motor component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,967,130	1	.	C	100.0%	56.6 / NA	16	A103G (GCC→GGC)	cheY	chemotaxis regulator transmitting signal to flagellar motor component
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (6/10);  total (6/10)

CCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGG‑CCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCAT  >  NC_000913/1966994‑1967247                                                                                                                                          |                                                                                                                      ccAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGG‑cc                                                                                                                     >  2:165078/1‑139 (MQ=255) ccAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATACTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGATAAATGGCTTCACCACATAGCCACTGG‑cc                                                                                                                     >  2:445329/1‑139 (MQ=255)  cAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGG‑ccc                                                                                                                    <  1:21776/139‑1 (MQ=255)             tCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTgcgc                                                                                                          <  2:80186/139‑1 (MQ=255)                        tttGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAatga                                                                                               <  1:14952/139‑1 (MQ=255)                              tgatGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTtct                                                                                         <  2:276971/139‑1 (MQ=255)                                               tCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCa                                                                        >  1:307394/1‑139 (MQ=255)                                                cGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAg                                                                       <  1:165078/139‑1 (MQ=255)                                                  cATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTc                                                                     >  1:48982/1‑139 (MQ=255)                                                  cATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTc                                                                     >  1:422034/1‑139 (MQ=255)                                                    tCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTctct                                                                                       <  2:199456/119‑1 (MQ=255)                                                    tCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTctct                                                                                       >  1:199456/1‑119 (MQ=255)                                                               ccAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCAtt                                                               <  1:635688/132‑1 (MQ=255)                                                               ccAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCAtt                                                               >  2:635688/1‑132 (MQ=255)                                                                                ttgttgAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATcgcg                                       <  1:445329/139‑1 (MQ=255)                                                                                                       cgcgGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAg                <  2:422034/139‑1 (MQ=255)                                                                                                       cgcgGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAg                <  2:48982/139‑1 (MQ=255)                                                                                                                     gCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGccgt  <  2:307394/139‑3 (MQ=255)                                                                                                                     gCTTCACCACATAGCCACTGGCCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGccat  >  1:275351/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                      CCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGG‑CCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCAT  >  NC_000913/1966994‑1967247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑