Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 281,050 (G)5→6 coding (934/1155 nt) yagA ← CP4‑6 prophage, putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,0451.G100.0% 95.4 / NA 27P313P (CCG→CCCyagACP4‑6 prophage, putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (13/14);  total (13/14)

GTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGT  >  NC_000913/280916‑281177
                                                                                                                                  |                                                                                                                                    
gTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCTCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCggggggcgt                                                                                                                             <  1:433144/139‑5 (MQ=255)
        gCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGc                                                                                                                      >  1:92346/1‑139 (MQ=255)
                          cccGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTg                                                                                                    <  1:173756/139‑1 (MQ=255)
                              ttCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACgg                                                                                                >  2:509142/1‑139 (MQ=255)
                                        aaCGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTgtgt                                                                                                                         <  2:80997/104‑1 (MQ=255)
                                        aaCGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTgtgt                                                                                                                         >  1:80997/1‑104 (MQ=255)
                                               tGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCg                                                                               >  2:527188/1‑139 (MQ=255)
                                                gCCTGCGCTCAGACTTACCCCTTTCCCGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCgg                                                                              <  1:37674/139‑1 (MQ=255)
                                                gCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCgg                                                                              <  1:509142/139‑1 (MQ=255)
                                                               tACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAg                                                               <  1:527188/139‑1 (MQ=255)
                                                                aCCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGcc                                                                                 >  2:5623/1‑120 (MQ=255)
                                                                aCCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGcc                                                                                 <  1:5623/120‑1 (MQ=255)
                                                                aCCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgc                                                              >  1:316685/1‑139 (MQ=255)
                                                                      ttCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAg                                                                                   <  1:327045/112‑1 (MQ=255)
                                                                      ttCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAg                                                                                   >  2:327045/1‑112 (MQ=255)
                                                                      ttCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCg                                                        >  1:649675/1‑139 (MQ=255)
                                                                               aGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGt                                               >  2:555930/1‑139 (MQ=255)
                                                                                  tttCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTATTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa                                            <  1:269484/139‑1 (MQ=255)
                                                                                  tttCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa                                            <  2:92346/139‑1 (MQ=255)
                                                                                  tttCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa                                            <  2:217538/139‑1 (MQ=255)
                                                                                    tCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGc                                                                                                                      <  2:272935/63‑1 (MQ=255)
                                                                                    tCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGc                                                                                                                      >  1:272935/1‑63 (MQ=255)
                                                                                                               cACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGcc                                                                                 <  1:638704/73‑1 (MQ=255)
                                                                                                               cACCCCTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGcc                                                                                 >  2:638704/1‑73 (MQ=255)
                                                                                                                   ccTTCATCGTATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGgcg           <  1:555930/139‑1 (MQ=255)
                                                                                                                            tATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAcc                                <  2:221113/109‑1 (MQ=255)
                                                                                                                            tATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAcc                                >  1:221113/1‑109 (MQ=255)
                                                                                                                            tATTCCGGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGt  >  2:250364/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                    
GTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGT  >  NC_000913/280916‑281177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: