Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	776,722	C→T	A127A (GCC→GCT)	tolA →	membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	776,722	0	C	T	100.0%	42.3 / NA	14	A127A (GCC→GCT)	tolA	membrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/9);  total (5/9)

ATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGC  >  NC_000913/776588‑776805                                                                                                                                       |                                                                                    aTGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCTGAGt                                                                                 <  1:131349/139‑1 (MQ=255)      ggAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCTGAGTTAAAg                                                                            <  2:335462/139‑1 (MQ=255)          cagcagGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGTAGGCTGAAGAAGCCGCAAAACAGGCAGAGTTAAAGgaga                                                                        >  1:543625/1‑139 (MQ=255)                                            cGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCggcggc                                      <  2:292171/139‑1 (MQ=255)                                             ggCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCa                                     <  1:507210/139‑1 (MQ=255)                                                                  gCAACGTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGaa                <  2:543625/139‑1 (MQ=255)                                                                                agCGGTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGc  >  2:502559/1‑139 (MQ=255)                                                                                    gTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCa             <  1:55535/124‑1 (MQ=255)                                                                                    gTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCa             >  2:55535/1‑124 (MQ=255)                                                                                                           aGCAGGCTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGcc                   >  1:327286/1‑95 (MQ=255)                                                                                                           aGCAGGCTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGcc                   <  2:327286/95‑1 (MQ=255)                                                                                                                 cTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCa                                     >  1:503408/1‑71 (MQ=255)                                                                                                                 cTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCa                                     <  2:503408/71‑1 (MQ=255)                                                                                                                 cTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCg                         >  1:635360/1‑83 (MQ=255)                                                                                                                 cTGAAGAAGCCGCAAAACAGGCTGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCg                         <  2:635360/83‑1 (MQ=255)                                                                                                                                       |                                                                                    ATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAGGCTGAAGAAGCCGCAAAACAGGCCGAGTTAAAGCAGAAGCAAGCTGAAGAGGCGGCAGCGAAAGCGGCGGCAGATGCTAAAGCGAAGGCCGAAGCAGATGCTAAAGC  >  NC_000913/776588‑776805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑