Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 924,093 A→G D277G (GAC→GGC)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913924,0930AG100.0% 83.9 / NA 23D277G (GAC→GGC) clpAATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/12);  total (11/12)

CAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGA  >  NC_000913/923960‑924227
                                                                                                                                     |                                                                                                                                      
cAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTa                                                                                                                                   <  2:654966/139‑1 (MQ=255)
                    gATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCt                                                                                                                         <  1:308585/129‑1 (MQ=255)
                    gATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCt                                                                                                                         >  2:487954/1‑129 (MQ=255)
                    gATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCt                                                                                                                         >  2:308585/1‑129 (MQ=255)
                    gATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCt                                                                                                                         <  1:487954/129‑1 (MQ=255)
                       ggCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATgag                                                                                                            <  2:299644/139‑1 (MQ=255)
                             cTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCcac                                                                                                      <  2:219921/139‑1 (MQ=255)
                                          tctctcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTg                                                                                         >  1:457901/1‑139 (MQ=255)
                                          tctctcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTg                                                                                         <  2:457901/139‑1 (MQ=255)
                                               cGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATcc                                                                                                        <  1:499494/119‑1 (MQ=255)
                                               cGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATcc                                                                                                        >  2:499494/1‑119 (MQ=255)
                                                                  gCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAgg                                                                 <  2:139626/139‑1 (MQ=255)
                                                                       cacaAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGAt                                                            >  2:608451/1‑139 (MQ=255)
                                                                                    ggcgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTaa                                               >  1:205292/1‑139 (MQ=255)
                                                                                               aaaaCGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTg                                    <  1:88236/139‑1 (MQ=255)
                                                                                                        tAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGc                           >  2:27108/1‑139 (MQ=255)
                                                                                                        tAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGc                           >  1:47304/1‑139 (MQ=255)
                                                                                                               ttGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                           >  2:442341/1‑36 (MQ=255)
                                                                                                               ttGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                           <  1:442341/36‑1 (MQ=255)
                                                                                                               ttGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGGGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCGAATCAAACCGTTGCTCTCCAGCGGTagaa                    >  1:417724/1‑139 (MQ=255)
                                                                                                                           cTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGAt                                                                                                          >  1:73403/1‑41 (MQ=255)
                                                                                                                           cTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGAt                                                                                                          <  2:73403/41‑1 (MQ=255)
                                                                                                                                 cAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGa  <  2:46783/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
CAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGA  >  NC_000913/923960‑924227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: