Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 986,267 C→T G239D (GGT→GAT)  ompF ← outer membrane porin 1a (Ia,b,F)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913986,2670CT100.0% 58.6 / NA 18G239D (GGT→GAT) ompFouter membrane porin 1a (Ia,b,F)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/8);  total (10/8)

CAACTAACAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCG  >  NC_000913/986136‑986377
                                                                                                                                   |                                                                                                              
cAACTAACAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGc                                                                                                         >  2:535381/1‑139 (MQ=255)
              tCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGAGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCtt                                                                                           >  1:137960/1‑139 (MQ=255)
                        tgttgGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGtt                                                                                 >  1:395873/1‑139 (MQ=255)
                           tgGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGcc                                                                              <  2:140002/139‑1 (MQ=255)
                                        ggTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGct                                                                 <  1:619535/139‑1 (MQ=255)
                                                 aaaTTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCACTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAgg                                                        <  2:111353/139‑1 (MQ=255)
                                                  aaTTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGt                                                       <  2:290703/139‑1 (MQ=255)
                                                              aTCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAg                                           >  2:70091/1‑139 (MQ=255)
                                                                  gCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGc                                       >  1:412386/1‑139 (MQ=255)
                                                                                           gTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTAcc                                                                                    <  1:576699/69‑1 (MQ=255)
                                                                                           gTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTAcc                                                                                    >  2:576699/1‑69 (MQ=255)
                                                                                            ttCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGc             >  2:531895/1‑139 (MQ=255)
                                                                                              cGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCt           >  1:115623/1‑139 (MQ=255)
                                                                                              cGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCt           >  2:90930/1‑139 (MQ=255)
                                                                                                       gTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCttcgtattcg  <  1:531895/139‑1 (MQ=255)
                                                                                                       gTAGATGTTGTTCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCttcgtattcg  <  2:395873/139‑1 (MQ=255)
                                                                                                             gttgttCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGc                                       >  1:522531/1‑96 (MQ=255)
                                                                                                             gttgttCGCGTCGTACTTCAGATCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGc                                       <  2:522531/96‑1 (MQ=255)
                                                                                                                                   |                                                                                                              
CAACTAACAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCG  >  NC_000913/986136‑986377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: