Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,934	A→G	I225T (ATC→ACC)	pyrC ←	dihydro‑orotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,934	0	A	G	100.0%	85.3 / NA	23	I225T (ATC→ACC)	pyrC	dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/12);  total (11/12)

AAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTG  >  NC_000913/1121799‑1122068                                                                                                                                        |                                                                                                                                       aaGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTgg                                                                                                                                     <  2:400364/139‑1 (MQ=255) aaGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGAGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTgg                                                                                                                                     <  1:483465/139‑1 (MQ=255)  agcagcCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTggg                                                                                                                                    <  2:182763/139‑1 (MQ=255)   gcagcCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAgggtgggt                                                                                                                                   >  2:120003/1‑139 (MQ=255)    cagcCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCACTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTa                                                                                                                                  <  1:464038/139‑1 (MQ=255)        ccGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACa                                                                                                                              >  2:215176/1‑139 (MQ=255)         cgcgCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACaa                                                                                                                             >  2:7897/1‑139 (MQ=255)         cgcgCAGCCACAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACaa                                                                                                                             >  1:92411/1‑139 (MQ=255)           cgcAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATa                                                                                                                           <  1:535223/139‑1 (MQ=255)                       tGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGact                                                                                                               <  1:411044/139‑2 (MQ=255)                          tctctTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGacgcac                                                                                                            >  2:32208/1‑139 (MQ=255)                                   gATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   <  2:92411/139‑1 (MQ=255)                                     tGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCa                                                                                                 >  2:194899/1‑139 (MQ=255)                                                                 cGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGAtg                                                                     <  1:120003/139‑1 (MQ=255)                                                                    ggAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGAtgctg                                                                  <  1:415174/139‑1 (MQ=255)                                                                                                  gTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCAtt                                    >  1:138325/1‑139 (MQ=255)                                                                                                            tGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACgg                          >  2:125455/1‑139 (MQ=255)                                                                                                             gCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGa                         >  1:370307/1‑139 (MQ=255)                                                                                                             gCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAAACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGa                         >  1:251560/1‑139 (MQ=255)                                                                                                                          tatTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGgcagc            <  1:7897/139‑1 (MQ=255)                                                                                                                               cGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCtt       <  1:194899/139‑1 (MQ=255)                                                                                                                                    gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCa                                                                                                 <  1:42884/44‑1 (MQ=255)                                                                                                                                    gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCa                                                                                                 >  2:42884/1‑44 (MQ=255)                                                                                                                                    gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTtggtg  >  2:585576/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       AAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTG  >  NC_000913/1121799‑1122068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑