Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	61,437	C→T	V610M (GTG→ATG)	rapA ←	RNA polymerase remodeling/recycling factor ATPase, RNA polymerase‑associated, ATP‑dependent RNA translocase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	61,437	0	C	T	100.0%	154.6 / NA	45	V610M (GTG→ATG)	rapA	RNA polymerase remodeling/recycling factor ATPase, RNA polymerase‑associated, ATP‑dependent RNA translocase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/21);  total (24/21)

ATCAGATCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGAT  >  NC_000913/61313‑61572                                                                                                                             |                                                                                                                                        aTCAGATCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAatat                                                                                                                           >  1:327052/1‑139 (MQ=255)      aTCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTgc                                                                                                                      >  1:472014/1‑139 (MQ=255)        cGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTgcgc                                                                                                                    >  2:515537/1‑139 (MQ=255)        cGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTgcgc                                                                                                                    >  2:465199/1‑139 (MQ=255)            gTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTgg                                                                                                                <  1:448084/139‑1 (MQ=255)             tataCGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGc                                                                                                               <  1:91595/139‑1 (MQ=255)                aCGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGa                                                                                                            >  1:464085/1‑139 (MQ=255)                    tATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                      <  1:14291/109‑1 (MQ=255)                    tATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                      >  2:14291/1‑109 (MQ=255)                     aTCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGa                                                                                                       >  1:493209/1‑139 (MQ=255)                          aaaTAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAg                                                                                                  <  2:245540/139‑1 (MQ=255)                            aTAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGTCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAgac                                                                                                <  1:465199/139‑1 (MQ=255)                            aTAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAgac                                                                                                <  1:515537/139‑1 (MQ=255)                            aTAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAgac                                                                                                <  2:57841/139‑1 (MQ=255)                                tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                      >  2:12029/1‑97 (MQ=255)                                tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                      >  2:233520/1‑97 (MQ=255)                                tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                      <  1:12029/97‑1 (MQ=255)                                tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATg                                                                                                                                      <  1:233520/97‑1 (MQ=255)                                tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGAcc                                                                                            >  1:346904/1‑139 (MQ=255)                                tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGAcc                                                                                            >  1:273616/1‑139 (MQ=255)                                tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGAcc                                                                                            >  2:464732/1‑139 (MQ=255)                                tGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGAcc                                                                                            >  2:138059/1‑139 (MQ=255)                                  cGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAg                                                                                                  >  1:1548/1‑131 (MQ=255)                                  cGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAg                                                                                                  <  2:1548/131‑1 (MQ=255)                                         tCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACgctg                                                                                   <  2:273616/139‑1 (MQ=255)                                               aGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAg                                                                             <  2:358232/139‑1 (MQ=255)                                                       tCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACgctgc                                                                                  <  1:309557/126‑1 (MQ=255)                                                       tCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACgctgc                                                                                  >  2:309557/1‑126 (MQ=255)                                                                tCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATg                                                            >  2:596590/1‑139 (MQ=255)                                                                     aCCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAgg                                                       <  2:472014/139‑1 (MQ=255)                                                                        ttCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCa                                                    <  2:464085/139‑1 (MQ=255)                                                                         tCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCaa                                                   >  2:79825/1‑139 (MQ=255)                                                                             gATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACAt                                               <  2:346904/139‑1 (MQ=255)                                                                                        aCCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGtggc                                    <  1:596590/139‑1 (MQ=255)                                                                                                 gACTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACt                           <  1:138059/139‑1 (MQ=255)                                                                                                  aCTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACgc                                                                                     >  2:244767/1‑80 (MQ=255)                                                                                                  aCTGAGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACgc                                                                                     <  1:244767/80‑1 (MQ=255)                                                                                                      aGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAg                      >  2:35464/1‑139 (MQ=255)                                                                                                      aGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAg                      >  1:514713/1‑139 (MQ=255)                                                                                                      aGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAg                      >  2:638336/1‑139 (MQ=255)                                                                                                      aGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAg                      >  1:116548/1‑139 (MQ=255)                                                                                                      aGCGGTTTTCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTCGCGAACTGGAAg                      >  1:141984/1‑139 (MQ=255)                                                                                                           ttttCTCCAGATAAGGCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACg                 <  2:514713/139‑1 (MQ=255)                                                                                                                         ggCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTCGCGAACTGGAAGTTACGTCCTTCAGAACCGa   <  2:141984/139‑1 (MQ=255)                                                                                                                          gCATATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGAt  >  2:128256/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                        ATCAGATCGTTGTATACGCTATCGTAAATAGTGCGTCCGGTCGGGCAGGTGTGCTCAAATGCATCCAGACCTTCGTGATACCAGCGCACCAGCACCGACTGAGCGGTTTTCTCCAGATAAGGCACATGGATCTGAATATCGTGCGCCTGGCCGATACGATCCAGACGACCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGAT  >  NC_000913/61313‑61572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑