Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,656,658	G→A	A159T (GCC→ACC)	speG →	spermidine N(1)‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,656,658	0	G	A	100.0%	142.6 / NA	42	A159T (GCC→ACC)	speG	spermidine N(1)‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/22);  total (20/22)

TCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGA  >  NC_000913/1656525‑1656772                                                                                                                                      |                                                                                                                   tctcAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCAtt                                                                                                               >  1:577006/1‑139 (MQ=255)     aaTCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCa                                                                                                           <  1:263704/139‑1 (MQ=255)                      tCTGATCGTTGATAAAGAGAATGACAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCAt                                                                                          <  2:367218/139‑1 (MQ=255)                          aTCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCAt                                                                                          <  2:452819/135‑1 (MQ=255)                          aTCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCAt                                                                                          >  1:452819/1‑135 (MQ=255)                              ttGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGTAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCAtt                                                                                                               <  1:158438/110‑1 (MQ=255)                              ttGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATCTCGTAATACCAtt                                                                                                               <  1:437739/110‑1 (MQ=255)                              ttGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCAtt                                                                                                               >  2:158438/1‑110 (MQ=255)                              ttGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCAtt                                                                                                               >  2:437739/1‑110 (MQ=255)                              ttGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCt                                                                                  >  2:53693/1‑139 (MQ=255)                              ttGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCt                                                                                  >  2:122898/1‑139 (MQ=255)                                        gaATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTcc                                                                                               <  1:473957/116‑1 (MQ=255)                                        gaATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTcc                                                                                               >  2:473957/1‑116 (MQ=255)                                         aaTGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCa                                                                                                                 >  1:189095/1‑97 (MQ=255)                                         aaTGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCa                                                                                                                 <  2:189095/97‑1 (MQ=255)                                         aaTGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGc                                                                                                            >  1:587826/1‑102 (MQ=255)                                         aaTGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGc                                                                                                            <  2:587826/102‑1 (MQ=255)                                         aaTGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCAt                                                                                                          <  1:38056/104‑1 (MQ=255)                                         aaTGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCAt                                                                                                          >  2:38056/1‑104 (MQ=255)                                         aaTGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCAt                                                                                          >  2:37220/1‑120 (MQ=255)                                         aaTGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCAt                                                                                          <  1:37220/120‑1 (MQ=255)                                                 aGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCAt                                                                                                          >  2:174230/1‑96 (MQ=255)                                                 aGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCAt                                                                                                          <  1:174230/96‑1 (MQ=255)                                                   cGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGc                                                                                                            <  2:448933/92‑1 (MQ=255)                                                   cGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGc                                                                                                            >  1:448933/1‑92 (MQ=255)                                                                     aGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCg                                                                                                             <  2:558107/73‑1 (MQ=255)                                                                     aGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCg                                                                                                             >  1:558107/1‑73 (MQ=255)                                                                        ttGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGAc                                        <  2:577006/139‑1 (MQ=255)                                                                                tCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATTTGTATATTCCAGCATCAGTATCTGGCa                                                                              <  1:392698/93‑1 (MQ=255)                                                                                tCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTggaa                                                                              >  2:392698/1‑91 (MQ=255)                                                                                                    gCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCg                                                                                                             <  2:368878/42‑1 (MQ=255)                                                                                                    gCACGAGTTCTTTATTAATGGTCAATATCGTAATACCATTCg                                                                                                             >  1:368878/1‑42 (MQ=255)                                                                                                             cTTTATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTg   >  2:355787/1‑139 (MQ=255)                                                                                                              tttATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGa  <  2:571966/139‑1 (MQ=255)                                                                                                              tttATTAATGGTCAATATCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGa  <  1:122898/139‑1 (MQ=255)                                                                                                              tttATTAATGGTCAATATCGTAATACCACTCGCATGTGTATATTCCACCATCGGTGTCGGGCACAGCACAAAACACCGGGTCAGACGCTCCTGCAGCCGTCCGCGACATAGCATTAATAATAATCGATGGCTTTTTTGa  <  1:431722/139‑1 (MQ=255)                                                                                                                            atatCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGa                                                        <  1:654920/71‑1 (MQ=255)                                                                                                                            atatCGTAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGa                                                        >  2:654920/1‑71 (MQ=255)                                                                                                                                  tAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAatcgatc            >  1:591463/1‑109 (MQ=255)                                                                                                                                  tAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAatcgatc            <  2:591463/109‑1 (MQ=255)                                                                                                                                  tAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGa  >  1:171214/1‑119 (MQ=255)                                                                                                                                  tAATACCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGa  <  2:171214/119‑1 (MQ=255)                                                                                                                                      |                                                                                                                   TCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGA  >  NC_000913/1656525‑1656772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑