Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,661,308 T→C R251R (CGT→CGC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,661,3080TC100.0% 121.9 / NA 33R251R (CGT→CGC) ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/18);  total (15/18)

TCGGTGCCAATGATGGCAACAGCCCGGATGATATCGCCAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGTTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGATTGCCTGGGTACTGATTACT  >  NC_000913/1661175‑1661440
                                                                                                                                     |                                                                                                                                    
tCGGTGCCAATGATGGCAACAGCCCGGATGATATCGCCAATACGAAACTGGTCGTTATGTTGGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCaaa                                                                                                                                 >  2:133338/1‑139 (MQ=255)
tCGGTGCCAATGATGGCAACAGCCCGGATGATATCGCCAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCaaa                                                                                                                                 >  2:200029/1‑139 (MQ=255)
 cGGTGCCAATGATGGCAACAGCCCGGATGATATCGCCAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAac                                                                                                                                >  2:305291/1‑139 (MQ=255)
  ggTGCCAATGATGGCAACAGCCCGGATGATATCGCCAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAacg                                                                                                                               <  2:414337/139‑1 (MQ=255)
   gTGCCAATGATGGCAACAGCCCGGATGATATCGCCAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAacgc                                                                                                                              <  2:573382/139‑1 (MQ=255)
    tGCTAATGATGGCAACAGCCCGGATGATATCGCCAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGCGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAacgca                                                                                                                             <  1:582347/139‑1 (MQ=255)
     gCCAATGATGGCAACAGCCCGGATGATATCGCCAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAacgcac                                                                                                                            >  1:78584/1‑139 (MQ=255)
                       ccGGATGATATCGCCAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATc                                                                                                          <  1:276528/139‑1 (MQ=255)
                          gatgatATCGCCAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCAc                                                                                                       >  1:444288/1‑139 (MQ=255)
                                    ccAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGa                                                                                             <  2:366745/139‑1 (MQ=255)
                                      aaTACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGaca                                                                                           <  2:530177/139‑1 (MQ=255)
                                                    cGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGt                                                                             <  2:78584/139‑1 (MQ=255)
                                                                          gCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCAc                                                                                                       <  2:9788/91‑1 (MQ=255)
                                                                          gCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCAc                                                                                                       >  1:9788/1‑91 (MQ=255)
                                                                          gCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTc                                                       >  2:614245/1‑139 (MQ=255)
                                                                             gAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGcc                                                    <  2:65434/139‑1 (MQ=255)
                                                                                       tGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGa                                          <  1:26794/139‑1 (MQ=255)
                                                                                            ggcggTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGgcg                                     >  1:231954/1‑139 (MQ=255)
                                                                                              cggTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCa                                   <  1:305291/139‑1 (MQ=255)
                                                                                              cggTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCa                                   <  1:200029/139‑1 (MQ=255)
                                                                                                gtggtgTCACTTACTACGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACt                                 >  1:14730/1‑139 (MQ=255)
                                                                                                           tactacGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGa                      >  2:452196/1‑139 (MQ=255)
                                                                                                                cGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGa                      >  2:191854/1‑134 (MQ=255)
                                                                                                                cGTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGa                      <  1:191854/134‑1 (MQ=255)
                                                                                                                 gTCGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGATTGCCt                <  1:133338/139‑1 (MQ=255)
                                                                                                                   cGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCAc                                                                                                       <  1:355468/50‑1 (MQ=255)
                                                                                                                   cGAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCAc                                                                                                       >  2:355468/1‑50 (MQ=255)
                                                                                                                    gAGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGATTGCCTggg             >  1:220127/1‑139 (MQ=255)
                                                                                                                     aGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGATTGCCTGGGt            >  1:208788/1‑139 (MQ=255)
                                                                                                                     aGCAAGCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGATTGCCTGGGt            >  2:23395/1‑139 (MQ=255)
                                                                                                                          gCCCGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGATTGCCTGGGTACTGa       <  1:614245/139‑1 (MQ=255)
                                                                                                                           cccGCGAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGATTGCCTGGGTACTGAt      <  2:444288/139‑1 (MQ=255)
                                                                                                                               cgAACGCTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGATTGCCTGGGTACTGATTACt  <  2:208788/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TCGGTGCCAATGATGGCAACAGCCCGGATGATATCGCCAATACGAAACTGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGCGGTGGTGTCACTTACTACGTCGAGCAAGCCCGCGAACGTTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACTGCTGCCGGGCGTGAAGATGAATGGCTGCCCATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGATTGCCTGGGTACTGATTACT  >  NC_000913/1661175‑1661440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑