Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,757,108	T→C	*471Q (TAA→CAA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,757,108	0	T	C	100.0%	83.6 / NA	23	*471Q (TAA→CAA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/11);  total (12/11)

TTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTAT  >  NC_000913/1756982‑1757229                                                                                                                               |                                                                                                                          ttCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCtttt                                                                                                               >  2:2168/1‑139 (MQ=255)     aCCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACTTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTACCACCGCAGCTGTTCACGCCCTGCAATATTGCTTTTGTGa                                                                                                           <  1:290420/139‑1 (MQ=255)        gTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGaatt                                                                                                        <  1:78057/139‑1 (MQ=255)           tGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGaattaat                                                                                                     <  1:175806/139‑1 (MQ=255)                   gAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTatat                                                                                             >  1:417160/1‑139 (MQ=255)                       tGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGaa                                                                                         <  2:429190/139‑1 (MQ=255)                              gCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGaattaat                                                                                                     <  2:567880/120‑1 (MQ=255)                              gCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGaattaat                                                                                                     >  1:567880/1‑120 (MQ=255)                                                             gTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGACTTAATTTGTATATCGAAGCGCCCTGATGGGCGATTTTTTTATTTAATCGATAAcc                                                   >  1:223739/1‑139 (MQ=255)                                                             gTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAAcc                                                   >  2:478756/1‑139 (MQ=255)                                                                         tCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATaaa                                       >  1:42445/1‑139 (MQ=255)                                                                                         cGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGAttt                       <  2:42445/139‑1 (MQ=255)                                                                                          gAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTc                      <  1:478756/139‑1 (MQ=255)                                                                                          gAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTc                      >  2:33707/1‑139 (MQ=255)                                                                                              ggCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAGTTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACta                  <  2:417160/139‑1 (MQ=255)                                                                                                          actgCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCACTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACt      <  1:33707/136‑1 (MQ=255)                                                                                                              cATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTAt  >  2:33685/1‑139 (MQ=255)                                                                                                              cATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTAt  >  1:178476/1‑139 (MQ=255)                                                                                                              cATCTGTTCACGTCCTGCAATATTGCTATTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGAATTTCACTATATAATCTCACTTTAt  >  2:425403/1‑139 (MQ=255)                                                                                                                    ttCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGa                                                                                          <  1:239050/45‑1 (MQ=255)                                                                                                                    ttCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGa                                                                                          >  2:239050/1‑45 (MQ=255)                                                                                                                         gTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgcg                                                                          >  1:370542/1‑56 (MQ=255)                                                                                                                         gTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgcg                                                                          <  2:370542/56‑1 (MQ=255)                                                                                                                               |                                                                                                                          TTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTAT  >  NC_000913/1756982‑1757229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑