| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 92,846 | C→T | G478G (GGC→GGT) | ftsI → | transpeptidase involved in septal peptidoglycan synthesis, penicillin‑binding protein 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 92,846 | 0 | C | T | 100.0% | 90.2 / NA | 27 | G478G (GGC→GGT) | ftsI | transpeptidase involved in septal peptidoglycan synthesis, penicillin‑binding protein 3 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/15); total (12/15) | |||||||||||
ACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGC > NC_000913/92712‑92974 | aCTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAggtggcg < 2:22690/139‑1 (MQ=255) ggCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAggcgg < 1:512105/139‑1 (MQ=255) ttATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGAtt < 2:235528/139‑1 (MQ=255) tATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTa > 2:491271/1‑139 (MQ=255) aTTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtatcg > 1:513857/1‑139 (MQ=255) cAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCAtt < 2:256636/139‑1 (MQ=255) cAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCAtt < 2:513857/139‑1 (MQ=255) gACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCg > 2:485136/1‑139 (MQ=255) ggTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAgg < 1:613273/139‑1 (MQ=255) aCGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAggtggcgg > 2:57/1‑72 (MQ=255) aCGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAggtggcgg < 1:57/72‑1 (MQ=255) tCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTCTCGCCATTAAAACCGGTACCGCGAAACAGGTCGGGCCGGACGGACGCTACa < 2:403152/139‑1 (MQ=255) cATTGTCCGCACTGTGGTGCATATTATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATc < 2:295847/139‑1 (MQ=255) tGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCACTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaat < 2:412907/139‑1 (MQ=255) gTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaata < 1:491271/139‑1 (MQ=255) gtggtgCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTg > 2:264838/1‑139 (MQ=255) tggtgCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaataa > 1:522562/1‑130 (MQ=255) tggtgCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaataa < 2:522562/130‑1 (MQ=255) tggtgCATATGATGGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGc > 1:317519/1‑139 (MQ=255) gatgGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCTTTAAAACCGGTACCGCGAAAAAgg < 1:446001/92‑1 (MQ=255) gatgGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAgg > 2:446001/1‑92 (MQ=255) gatgGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAgg > 2:841/1‑92 (MQ=255) gatgGAAAGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAgg < 1:841/92‑1 (MQ=255) aGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaat > 2:167444/1‑111 (MQ=255) aGCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaat < 1:167444/111‑1 (MQ=255) gCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCg > 2:16652/1‑129 (MQ=255) gCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCg < 1:16652/129‑1 (MQ=255) gCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCa > 2:192987/1‑131 (MQ=255) gCGTGGCGCTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCa < 1:192987/131‑1 (MQ=255) cgcTACCAGGTGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGc > 2:570090/1‑139 (MQ=255) | ACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGC > NC_000913/92712‑92974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |