Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,482,862	A→G	L160L (TTA→CTA)	emrK ←	multidrug resistance efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,482,862	0	A	G	100.0%	116.8 / NA	32	L160L (TTA→CTA)	emrK	multidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

GCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTAT  >  NC_000913/2482726‑2482996                                                                                                                                         |                                                                                                                                       gcCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCg                                                                                                                                      <  1:259880/139‑1 (MQ=255) gcCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCg                                                                                                                                      <  1:286544/139‑1 (MQ=255)           cttcttTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGt                                                                                                                            <  2:541308/139‑1 (MQ=255)               tttAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGc                                                                                                                        >  2:31882/1‑139 (MQ=255)                      gCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCg                                                                                                                 <  2:247592/139‑1 (MQ=255)                        aTCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGtt                                                                                                               >  1:422099/1‑139 (MQ=255)                                                                   ttCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGt                                                                    >  1:250857/1‑139 (MQ=255)                                                                             aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGt                                                                                                                >  1:315485/1‑85 (MQ=255)                                                                             aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGt                                                                                                                <  2:315485/85‑1 (MQ=255)                                                                             aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCg                                                                         >  1:276613/1‑124 (MQ=255)                                                                             aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCg                                                                         <  2:276613/124‑1 (MQ=255)                                                                             aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGa                                                                        <  2:528141/125‑1 (MQ=255)                                                                             aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGa                                                                        >  1:528141/1‑125 (MQ=255)                                                                             aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAAtcttc                                                          >  1:244391/1‑139 (MQ=255)                                                                             aaGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAAtcttc                                                          >  2:37881/1‑139 (MQ=255)                                                                                                  ccTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAAt                                     <  2:250857/139‑1 (MQ=255)                                                                                                    tGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATAc                                   >  1:514368/1‑139 (MQ=255)                                                                                                           cGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGAttgt                                                >  2:114691/1‑119 (MQ=255)                                                                                                           cGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGAttgt                                                <  1:114691/119‑1 (MQ=255)                                                                                                           cGGCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGa                            >  2:354848/1‑139 (MQ=255)                                                                                                            ggCATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGaa                           <  1:53626/139‑1 (MQ=255)                                                                                                              cATTCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGc                         <  2:514368/139‑1 (MQ=255)                                                                                                                ttCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTtata                                                                <  2:300977/98‑1 (MQ=255)                                                                                                                ttCAATGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTtata                                                                >  1:300977/1‑98 (MQ=255)                                                                                                                     tGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCg                  <  2:556497/139‑1 (MQ=255)                                                                                                                     tGCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCg                  >  1:556497/1‑139 (MQ=255)                                                                                                                      gCCGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCgg                 <  1:354848/139‑1 (MQ=255)                                                                                                                        cGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCa               <  2:172326/139‑1 (MQ=255)                                                                                                                        cGCTTTGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCa               >  2:119860/1‑139 (MQ=255)                                                                                                                           tttGCTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTg            >  2:407184/1‑139 (MQ=255)                                                                                                                               cTACTTATTAGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTAtt        >  1:339103/1‑139 (MQ=255)                                                                                                                                     attaGCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTAt  <  1:213205/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GCCAACCAGGCTTCTTTAGTTGCATCCGCCGCTTCAACGACTTGTGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTAT  >  NC_000913/2482726‑2482996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑