Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,634,449 G→A R73H (CGC→CAC)  xseA → exonuclease VII, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,634,4490GA100.0% 97.2 / NA 30R73H (CGC→CAC) xseAexonuclease VII, large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/15);  total (15/15)

GGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGAAGGGCTGCTGCAACAGAAGTA  >  NC_000913/2634318‑2634572
                                                                                                                                   |                                                                                                                           
ggTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAg                                                                                                                      >  1:628156/1‑139 (MQ=255)
           gcggcgAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGcaaca                                                                                                           >  2:311653/1‑139 (MQ=255)
           gcggcgAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGcaaca                                                                                                           >  1:447503/1‑139 (MQ=255)
                     ttCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGtt                                                                                                 <  2:336915/139‑1 (MQ=255)
                                  cAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTa                                                                                    <  1:536867/139‑1 (MQ=255)
                                                              cacTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCcaca                                                                                                                       <  1:128339/76‑1 (MQ=255)
                                                              cacTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCcaca                                                                                                                       >  2:128339/1‑76 (MQ=255)
                                                                  cAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACCCTCTACGAGCCGCGCGGCGACTACCAGATa                                                    <  1:351573/139‑1 (MQ=255)
                                                                   aaaGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATaa                                                   >  2:310731/1‑139 (MQ=255)
                                                                       acgacACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt                                               <  2:628156/139‑1 (MQ=255)
                                                                       acgacACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt                                               <  2:447503/139‑1 (MQ=255)
                                                                       acgacACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt                                               <  1:601863/139‑1 (MQ=255)
                                                                        cgacACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt                                               <  1:418490/138‑1 (MQ=255)
                                                                        cgacACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt                                               >  2:418490/1‑138 (MQ=255)
                                                                           cacCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTgag                                           <  2:563041/139‑1 (MQ=255)
                                                                           cacCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTgag                                           >  1:238040/1‑139 (MQ=255)
                                                                                     gTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAgc                                 >  2:315881/1‑139 (MQ=255)
                                                                                          cTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATggg                                                                                                                <  1:404011/55‑1 (MQ=255)
                                                                                          cTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATggg                                                                                                                >  2:404011/1‑55 (MQ=255)
                                                                                          cTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt                                               >  1:561595/1‑120 (MQ=255)
                                                                                          cTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt                                               <  2:561595/120‑1 (MQ=255)
                                                                                          cTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAgccggcc                            >  1:434330/1‑139 (MQ=255)
                                                                                           tGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCgcgc                                                                                            >  1:189961/1‑74 (MQ=255)
                                                                                           tGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCgcgc                                                                                            <  2:189961/74‑1 (MQ=255)
                                                                                             cgcgATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCgcggc                                                                <  1:402542/100‑1 (MQ=255)
                                                                                             cgcgATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCgcggc                                                                >  2:402542/1‑100 (MQ=255)
                                                                                               cgATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGa                       >  2:282663/1‑139 (MQ=255)
                                                                                                              gCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGAAGGGCTGCTGCAACa        <  1:282663/139‑1 (MQ=255)
                                                                                                              gCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGAAGGGCTGCTGCAACa        <  2:434330/139‑1 (MQ=255)
                                                                                                                    gccgGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGAAGGGCTGCTGCAACAGAAGTa  >  1:557551/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                           
GGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGAAGGGCTGCTGCAACAGAAGTA  >  NC_000913/2634318‑2634572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: