Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,899,230	T→C	V63V (GTA→GTG)	ygcU ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,899,230	0	T	C	100.0%	79.2 / NA	23	V63V (GTA→GTG)	ygcU	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/11);  total (12/11)

TGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACG  >  NC_000913/2899106‑2899359                                                                                                                             |                                                                                                                                  tGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGa                                                                                                                     >  2:187903/1‑139 (MQ=255)       aGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCCAg                                                                                                               >  1:438195/1‑139 (MQ=255)            tCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTa                                                                                                          >  1:296202/1‑139 (MQ=255)            tCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTa                                                                                                          >  2:286214/1‑139 (MQ=255)                gCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGAc                                                                                                      <  1:142434/139‑1 (MQ=255)                              tCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCg                                                                                        >  1:334142/1‑139 (MQ=255)                                 acaacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCa                                                                                     <  1:377527/139‑1 (MQ=255)                                     caGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGAc                                                                                                      <  2:572752/118‑1 (MQ=255)                                     caGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGAc                                                                                                      >  1:572752/1‑118 (MQ=255)                                        tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCg                                                                                                                 >  1:100900/1‑104 (MQ=255)                                        tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCg                                                                                                                 <  2:100900/104‑1 (MQ=255)                                        tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtata                                                                              >  1:163903/1‑139 (MQ=255)                                                         gTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCg                                                             >  1:619725/1‑139 (MQ=255)                                                                aaGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAAttt                                                      >  1:193259/1‑139 (MQ=255)                                                                    aCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGa                                                  >  2:655271/1‑139 (MQ=255)                                                                      cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAGTATCCGGAAATTTACGaaa                                                <  2:438195/139‑1 (MQ=255)                                                                      cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa                                                <  2:619725/139‑1 (MQ=255)                                                                           cgcgGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt                                           <  1:349400/139‑1 (MQ=255)                                                                                      gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                <  1:655271/139‑1 (MQ=255)                                                                                      gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                <  1:36959/139‑1 (MQ=255)                                                                                                 gcgcATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATAc                     >  2:638680/1‑139 (MQ=255)                                                                                                     aTTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGtt                 <  2:163903/139‑1 (MQ=255)                                                                                                                    cacaCGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTAcg  <  2:334142/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                  TGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACG  >  NC_000913/2899106‑2899359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑