Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,931,839 T→C intergenic (+29/+26) ygdG → / ← fucO Ssb‑binding protein, misidentified as ExoIX/L‑1,2‑propanediol oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,931,8390TC100.0% 73.8 / NA 23intergenic (+29/+26)ygdG/fucOSsb‑binding protein, misidentified as ExoIX/L‑1,2‑propanediol oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/15);  total (8/15)

AAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATG  >  NC_000913/2931703‑2931970
                                                                                                                                        |                                                                                                                                   
aaaTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCAt                                                                                                                                   <  2:235592/139‑1 (MQ=255)
                aaGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATc                                                                                                                   <  2:367275/139‑1 (MQ=255)
                               tgtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAgg                                                                                                    <  2:136450/139‑1 (MQ=255)
                               tgtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAgg                                                                                                    <  1:429255/139‑1 (MQ=255)
                               tgtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAgg                                                                                                    <  1:472522/139‑1 (MQ=255)
                               tgtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAgg                                                                                                    <  1:490667/139‑1 (MQ=255)
                               tgtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCAGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAgg                                                                                                    <  2:136451/139‑1 (MQ=255)
                                              cccGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGct                                                                                     <  1:47173/139‑1 (MQ=255)
                                                   ttACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCCTTCGGCATTATCCCATCAGCGCATTTACCCGGCGGTATGGTGAAGCTCTACa                                                                                >  1:275764/1‑139 (MQ=255)
                                                   ttACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACa                                                                                >  2:508427/1‑139 (MQ=255)
                                                     aCAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAat                                                                              >  1:398653/1‑139 (MQ=255)
                                                                                          ttGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCgg                                                                                                 <  2:284161/83‑1 (MQ=255)
                                                                                          ttGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCgg                                                                                                 >  1:284161/1‑83 (MQ=255)
                                                                                           tGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTAc                                        <  1:518651/139‑1 (MQ=255)
                                                                                                    tACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTc                                                                        >  1:29050/1‑98 (MQ=255)
                                                                                                    tACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTc                                                                        <  2:29050/98‑1 (MQ=255)
                                                                                                          aaCGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTaa                                                                                         <  1:352404/75‑1 (MQ=255)
                                                                                                          aaCGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTaa                                                                                         >  2:352404/1‑75 (MQ=255)
                                                                                                          aaCGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTg                         <  1:508427/139‑1 (MQ=255)
                                                                                                            cGGCGAGCCGGATACGCCGCAAACGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGATCTACAATATCCTCAAACGTTGCTTCACGCGGGATGCCACCGGTACAAAAATCATCCAGTgcc                       >  1:183668/1‑139 (MQ=255)
                                                                                                                                 aaCGTCGCATCCGGCATTATCACATCAGCGCATTTACCAgg                                                                                                    <  1:479016/41‑1 (MQ=255)
                                                                                                                                 aaCGTCGCATCCGGCATTATCACATCAGCGCATTTACCAgg                                                                                                    >  2:479016/1‑41 (MQ=255)
                                                                                                                                 aaCGTCGCATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATg  <  2:275764/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
AAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATG  >  NC_000913/2931703‑2931970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: