Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,097,854	G→A	L196L (CTG→CTA)	yggW →	HemN family putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,097,854	0	G	A	100.0%	108.7 / NA	32	L196L (CTG→CTA)	yggW	HemN family putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/14);  total (18/14)

GATTTGATGCATGGGCTGCCGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCT  >  NC_000913/3097753‑3097986                                                                                                      |                                                                                                                                     gatttgatGCATGGGCTGCCGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGaccac                                                                                                 >  1:383779/1‑139 (MQ=255)            tGGGCTGCCGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCg                                                                                      >  2:47343/1‑139 (MQ=255)                    cGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATa                                                                                                                      <  2:167101/99‑1 (MQ=255)                    cGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATa                                                                                                                      >  1:167101/1‑99 (MQ=255)                    cGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGtg                                                                                            <  2:372012/125‑1 (MQ=255)                    cGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGtg                                                                                            >  1:372012/1‑125 (MQ=255)                                tGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGa                                                                  <  1:47343/139‑1 (MQ=255)                                  gAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAAC‑AACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGatat                                                               >  1:367522/1‑139 (MQ=255)                                          gcTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGaa                                                        >  1:574029/1‑139 (MQ=255)                                          gcTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGaa                                                        >  2:446420/1‑139 (MQ=255)                                                   tCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCAt                                               <  1:32928/139‑1 (MQ=255)                                                      aCGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAg                                            <  2:574029/139‑1 (MQ=255)                                                             gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGt                                                                                             <  2:154148/83‑1 (MQ=255)                                                             gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGAc                                                                             <  1:170302/99‑1 (MQ=255)                                                             gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGAc                                                                             >  2:170302/1‑99 (MQ=255)                                                             gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATAtt                                                            <  1:612595/116‑1 (MQ=255)                                                             gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATAtt                                                            >  2:612595/1‑116 (MQ=255)                                                             gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTaa                                     >  1:638346/1‑139 (MQ=255)                                                             gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTaa                                     >  1:557485/1‑139 (MQ=255)                                                             gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCCGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTaa                                     >  2:568032/1‑139 (MQ=255)                                                             gCCACTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGt                                                                                             >  1:154148/1‑83 (MQ=255)                                                                       tGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCggg                           >  1:42293/1‑139 (MQ=255)                                                                       tGAATCCGCCGCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCggg                           <  2:557485/139‑1 (MQ=255)                                                                            ccgccgCATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATc                      <  1:446420/139‑1 (MQ=255)                                                                              gccgcATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAg                    <  2:42293/139‑1 (MQ=255)                                                                                cgcATCTTTCCTGGTATCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCa                  >  1:146399/1‑139 (MQ=255)                                                                                               aTCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGAcgcg                                                                          <  1:56820/68‑1 (MQ=255)                                                                                               aTCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGAcgcg                                                                          >  2:56820/1‑68 (MQ=255)                                                                                                tCAACTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCt  >  1:535886/1‑139 (MQ=255)                                                                                                tCAACTAACCATCGAACCCAATACGCCGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCt  <  1:568032/139‑1 (MQ=255)                                                                                                  aaCTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCAt                                               <  1:560339/92‑1 (MQ=255)                                                                                                  aaCTAACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCAt                                               >  2:560339/1‑92 (MQ=255)                                                                                                      |                                                                                                                                     GATTTGATGCATGGGCTGCCGGATCAATCACTGGAAGAGGCGCTTGGCGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCT  >  NC_000913/3097753‑3097986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑