Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,216,824 C→T R16C (CGC→TGC)  yqjI → PadR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,216,8240CT100.0% 114.2 / NA 34R16C (CGC→TGC) yqjIPadR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

CGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTCAC  >  NC_000913/3216695‑3216958
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cGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAgg                                                                                                                               >  2:495889/1‑139 (MQ=255)
                        gCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAg                                                                                                       <  2:552121/139‑1 (MQ=255)
                                gCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAg                                                                                               <  1:528902/139‑1 (MQ=255)
                                            tataAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGTGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAg                                                                                                       <  2:515091/119‑1 (MQ=255)
                                            tataAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGACAGCCATGCCATGAGGGCTGATGCAAAGGTGAGAAGTCAg                                                                                                       >  1:515091/1‑119 (MQ=255)
                                              taAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGctgctg                                                                                                                        <  2:203196/100‑1 (MQ=255)
                                              taAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGctgctg                                                                                                                        >  1:203196/1‑100 (MQ=255)
                                              taAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACgg                                                                                 >  2:47856/1‑139 (MQ=255)
                                                aaTTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCa                                                                                                                      <  1:131492/100‑1 (MQ=255)
                                                aaTTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCa                                                                                                                      >  2:131492/1‑100 (MQ=255)
                                                     gATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGaca                                                                                    <  2:451416/129‑1 (MQ=255)
                                                     gATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGaca                                                                                    >  1:451416/1‑129 (MQ=255)
                                                          atCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGaca                                                                                    >  1:277084/1‑124 (MQ=255)
                                                          atCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGaca                                                                                    <  2:277084/124‑1 (MQ=255)
                                                           tCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGaa                                                                    >  2:233998/1‑139 (MQ=255)
                                                            cTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAac                                                                   <  1:233998/139‑1 (MQ=255)
                                                                ttAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACgg                                                               >  2:416662/1‑139 (MQ=255)
                                                                   aGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCa                                                            <  1:47856/139‑1 (MQ=255)
                                                                     cAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAAt                                                          >  1:495152/1‑139 (MQ=255)
                                                                         aggaggCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAAtgctg                                                      >  1:146973/1‑139 (MQ=255)
                                                                             ggCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCggt                                                  >  2:142553/1‑139 (MQ=255)
                                                                                tGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCggtggt                                               <  2:548499/139‑1 (MQ=255)
                                                                                            tcatcaCGAAGGGTGTTGTAAGCATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCAcc                                                                            <  1:470371/98‑1 (MQ=255)
                                                                                            tcatcaCGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCAcc                                                                            >  2:470371/1‑98 (MQ=255)
                                                                                            tcatcaCGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCAcc                                                                            >  2:290204/1‑98 (MQ=255)
                                                                                            tcatcaCGAAGGGTGTTGTAAACATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCAcc                                                                            <  1:290204/98‑1 (MQ=255)
                                                                                                                 aCATGAAGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAAcat              >  1:125776/1‑137 (MQ=255)
                                                                                                                      aaGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTc                                                                                                         <  2:185065/43‑1 (MQ=255)
                                                                                                                      aaGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTc                                                                                                         >  1:185065/1‑43 (MQ=255)
                                                                                                                      aaGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAacac                                                                 <  2:618011/83‑1 (MQ=255)
                                                                                                                      aaGGCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAacac                                                                 >  1:618011/1‑83 (MQ=255)
                                                                                                                        ggCCAGCCATGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTg       >  1:552184/1‑139 (MQ=255)
                                                                                                                          ccagccaTGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGt     <  2:146973/139‑1 (MQ=255)
                                                                                                                            agccaTGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTCa   <  2:552184/139‑1 (MQ=255)
                                                                                                                             gccatgccatGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTCAc  >  2:326649/1‑139 (MQ=255)
                                                                                                                             gccatgccatGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTCAc  >  2:200557/1‑139 (MQ=255)
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CGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTCAC  >  NC_000913/3216695‑3216958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑