Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,048	G→A	R202C (CGC→TGC)	yhaM ←	putative L‑serine dehydratase alpha chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,048	0	G	A	100.0%	64.4 / NA	20	R202C (CGC→TGC)	yhaM	putative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/10);  total (10/10)

ATGAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAT  >  NC_000913/3255913‑3256176                                                                                                                                        |                                                                                                                                 aTGAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGAt                                                                                                                               >  1:33787/1‑139 (MQ=255) aTGAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGAt                                                                                                                               >  2:315595/1‑139 (MQ=255)   gAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATcg                                                                                                                             >  1:533600/1‑139 (MQ=255)          gaTCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATGTGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCaaa                                                                                                                      >  2:444270/1‑139 (MQ=255)           aTCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAAc                                                                                                                     >  1:301703/1‑139 (MQ=255)            tCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACg                                                                                                                    >  2:161419/1‑139 (MQ=255)                        aaaCCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGa                                                                                                        <  2:195731/139‑1 (MQ=255)                          aCCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAATGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACg                                                                                                      <  1:428541/139‑1 (MQ=255)                            cgcgCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGaa                                                                                                    <  2:533600/139‑1 (MQ=255)                                                aGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGcg                                                                                <  1:444270/139‑1 (MQ=255)                                                   gTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGcgtcg                                                                             >  1:593715/1‑139 (MQ=255)                                                                       ccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGc                                                         >  1:222311/1‑139 (MQ=255)                                                                                 cTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACg                                                                                                      <  1:6219/84‑1 (MQ=255)                                                                                 cTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACg                                                                                                      >  2:6219/1‑84 (MQ=255)                                                                                        cTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGcc                                        <  2:28765/139‑1 (MQ=255)                                                                                                     cGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGcc                                        <  1:582341/126‑1 (MQ=255)                                                                                                     cGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGcc                                        >  2:582341/1‑126 (MQ=255)                                                                                                           aTTTAGCTTCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgc                     <  1:161419/139‑1 (MQ=255)                                                                                                                   tCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTaaa             <  2:222311/139‑1 (MQ=255)                                                                                                                   tCGCGGAATCGAGAATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTaaa             <  1:315595/139‑1 (MQ=255)                                                                                                                              agaATAAAGCAGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAt  >  1:34170/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                 ATGAAGAGAGATCTTTCGCCAGCAAACCGCGCTCGCACTGTTTTTCCAGCGTCGCGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAT  >  NC_000913/3255913‑3256176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑