Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,347,929 T→C V272A (GTC→GCC)  yhbJ → adaptor protein for GlmZ/GlmY sRNA decay, glucosamine‑6‑phosphate‑regulated, NTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,347,9290TC100.0% 104.8 / NA 28V272A (GTC→GCC) yhbJadaptor protein for GlmZ/GlmY sRNA decay, glucosamine‑6‑phosphate‑regulated, NTPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/11);  total (17/11)

CTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCA  >  NC_000913/3347796‑3348048
                                                                                                                                     |                                                                                                                       
cTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGt                                                                                                                    >  1:140479/1‑139 (MQ=255)
                                       ttGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGt                                                                             >  1:430717/1‑139 (MQ=255)
                                       ttGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGt                                                                             >  1:365230/1‑139 (MQ=255)
                                             gTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCa                                                                       >  1:178727/1‑139 (MQ=255)
                                                      ggTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGa                                                              <  1:203405/139‑1 (MQ=255)
                                                            aCCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATcac                                                        >  2:396339/1‑139 (MQ=255)
                                                               ggcggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaa                                                     <  2:430717/139‑1 (MQ=255)
                                                               ggcggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaa                                                     <  2:365230/139‑1 (MQ=255)
                                                                                 gtgtATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAATCTGGGCatgcatgc                                   <  2:140479/139‑1 (MQ=255)
                                                                                  tgtATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGcc                                  <  2:178727/139‑1 (MQ=255)
                                                                                       atTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGcc                             >  2:240391/1‑139 (MQ=255)
                                                                                            agagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGc                                                                                                     >  2:327138/1‑62 (MQ=255)
                                                                                            agagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGc                                                                                                     <  1:327138/62‑1 (MQ=255)
                                                                                            agagCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCaa                        >  2:295839/1‑139 (MQ=255)
                                                                                              agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACg                                                                                                                <  1:215330/49‑1 (MQ=255)
                                                                                              agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACg                                                                                                                >  2:215330/1‑49 (MQ=255)
                                                                                              agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                      >  1:202214/1‑139 (MQ=255)
                                                                                              agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                      >  1:104769/1‑139 (MQ=255)
                                                                                              agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                      >  2:242881/1‑139 (MQ=255)
                                                                                                cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACCAACAAGCTGGGCATGCATGCCCGGCCTGCAATGaa                    >  2:151095/1‑139 (MQ=255)
                                                                                                cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGaa                    >  2:314854/1‑139 (MQ=255)
                                                                                                 aaCTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAg                   <  1:295839/139‑1 (MQ=255)
                                                                                                        aGACTACTTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTg            >  1:307974/1‑139 (MQ=255)
                                                                                                             actTCCGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTa       >  1:90723/1‑139 (MQ=255)
                                                                                                                  cGCTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCa  <  1:240391/139‑1 (MQ=255)
                                                                                                                    cTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                 <  1:578363/90‑1 (MQ=255)
                                                                                                                    cTCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                 >  2:578363/1‑90 (MQ=255)
                                                                                                                     tCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGaa          >  1:647906/1‑128 (MQ=255)
                                                                                                                     tCGCGCGGTAAAAACGCCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGaa          <  2:647906/128‑1 (MQ=255)
                                                                                                                                     |                                                                                                                       
CTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCA  >  NC_000913/3347796‑3348048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: