Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,450,674 G→A P231S (CCA→TCA)  rplB ← 50S ribosomal subunit protein L2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,450,6740GA100.0% 76.5 / NA 23P231S (CCA→TCA) rplB50S ribosomal subunit protein L2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/8);  total (15/8)

GCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGGGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTG  >  NC_000913/3450549‑3450808
                                                                                                                             |                                                                                                                                      
gCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCggg                                                                                                                           <  1:346257/139‑1 (MQ=255)
                             tACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACg                                                                                              >  1:65346/1‑139 (MQ=255)
                              aCGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGa                                                                                             >  2:553337/1‑139 (MQ=255)
                               cGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGaa                                                                                            >  2:297774/1‑139 (MQ=255)
                                    gttgCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAAcacca                                                                                       >  2:580959/1‑139 (MQ=255)
                                           cGGGTCTTCTTACCTTTGGTCTGAATGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACg                                                                                     <  1:222252/134‑1 (MQ=255)
                                           cGGGTCTTCTTACCTTTGGTCTGAATGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACg                                                                                     >  2:222252/1‑134 (MQ=255)
                                                            ggTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAAcacca                                                                                       <  1:203215/115‑1 (MQ=255)
                                                            ggTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAAcacca                                                                                       >  2:203215/1‑115 (MQ=255)
                                                             gTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTg                                                                             <  1:611716/124‑1 (MQ=255)
                                                             gTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTg                                                                             >  2:611716/1‑124 (MQ=255)
                                                                       cccACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcgcg                                                    >  1:576777/1‑139 (MQ=255)
                                                                       cccACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcgcg                                                    >  2:157860/1‑139 (MQ=255)
                                                                           cGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCgcagc                                                <  1:580959/139‑1 (MQ=255)
                                                                                 tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGCACgcgc                                                   >  2:459011/1‑130 (MQ=255)
                                                                                 tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACgcgc                                                   <  1:459011/130‑1 (MQ=255)
                                                                                 tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGc                                          >  1:42778/1‑139 (MQ=255)
                                                                                 tACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGAGTGGTCTACCGGGTTCAGCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGc                                          >  1:146063/1‑139 (MQ=255)
                                                                                                                    caccaTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCa                                       <  2:280098/107‑1 (MQ=255)
                                                                                                                    caccaTGTGAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCa                                       >  1:280098/1‑107 (MQ=255)
                                                                                                                         tgtgAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcgcg                                                    <  2:222950/89‑1 (MQ=255)
                                                                                                                         tgtgAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAAcgcg                                                    >  1:222950/1‑89 (MQ=255)
                                                                                                                         tgtgAGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTg  >  2:445178/1‑139 (MQ=255)
                                                                                                                             |                                                                                                                                      
GCTACGGCGACGTACGATGAATTTATCAGTACGCTTGTTGCTGCGGGTCTTCTTACCTTTGGTCTGAACGCCCCACGGAGTTACCGGGTGCTTACCAAAGTTACGACCTTCACCACCACCATGTGGGTGGTCTACCGGGTTCATCGCGGTACCGCGAACGGTCGGACGAACACCACGCCAGCGTGCAGCACCTGCTTTACCCAGAACGCGCAGCATATGCTCAGCATTGCCAACTTCGCCCAGAGTTGCACGGCAGTCTG  >  NC_000913/3450549‑3450808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: