| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 337,217 | T→C | V147A (GTC→GCC) | yahF → | putative NAD(P)‑binding succinyl‑CoA synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 337,217 | 0 | T | C | 94.4% | 54.9 / ‑3.8 | 18 | V147A (GTC→GCC) | yahF | putative NAD(P)‑binding succinyl‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (6/11); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-01 | |||||||||||
GCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACAT > NC_000913/337081‑337352 | gCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcct < 2:439148/139‑1 (MQ=255) cTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGt < 2:325438/139‑1 (MQ=255) cgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGaa > 1:231648/1‑139 (MQ=255) ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg < 2:489355/139‑1 (MQ=255) ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCACTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg < 1:26644/139‑1 (MQ=255) gTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgatga < 1:587825/139‑1 (MQ=255) gTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgatga < 2:231648/139‑1 (MQ=255) aCGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcct < 1:302185/78‑1 (MQ=255) gCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGt > 1:557396/1‑71 (MQ=255) gCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGt < 2:557396/71‑1 (MQ=255) cgTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGccc < 1:528234/88‑1 (MQ=255) cgTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGccc > 2:528234/1‑88 (MQ=255) gCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAAcggcgcggcg < 2:506287/139‑1 (MQ=255) aaaaCGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGct < 1:217649/139‑1 (MQ=255) aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCGGACTGTGGCACgg < 1:506863/100‑1 (MQ=255) aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACgg > 2:506863/1‑100 (MQ=255) tGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGt > 2:639023/1‑139 (MQ=255) ttttCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAAcggc < 1:86585/108‑1 (MQ=255) ttttCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAAcggc > 2:86585/1‑108 (MQ=255) aCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACAt < 2:73718/139‑1 (MQ=255) | GCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACAT > NC_000913/337081‑337352 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |