Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,485,541 C→T G93D (GGT→GAT)  yhfA ← OsmC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,485,5410CT97.1% 112.7 / NA 34G93D (GGT→GAT) yhfAOsmC family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/16);  minor base G (0/1);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCAC  >  NC_000913/3485411‑3485676
                                                                                                                                  |                                                                                                                                       
aaGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGt                                                                                                                                     <  1:120906/135‑1 (MQ=255)
aaGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGt                                                                                                                                     >  2:120906/1‑135 (MQ=255)
aaGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACg                                                                                                                                 >  1:617457/1‑139 (MQ=255)
   tCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATa                                                                                                                              >  1:338806/1‑139 (MQ=255)
       gcgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAAt                                                                                                                          >  1:304270/1‑139 (MQ=255)
        cgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACg                                                                                                                                 >  1:116699/1‑131 (MQ=255)
        cgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACg                                                                                                                                 <  2:116699/131‑1 (MQ=255)
        cgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATg                                                                                                                         >  1:200701/1‑139 (MQ=255)
        cgGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACAATAAAATg                                                                                                                         >  2:585836/1‑139 (MQ=255)
                aCTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTaa                                                                                                                 <  1:183158/139‑1 (MQ=255)
                         gagtgagtAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTaa                                                                                                        >  2:614112/1‑139 (MQ=255)
                            tgagtAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACa                                                                                                     >  2:26140/1‑139 (MQ=255)
                            tgagtAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACa                                                                                                     >  1:366468/1‑139 (MQ=255)
                                tAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCg                                                                                                 <  2:617457/139‑1 (MQ=255)
                                     ttCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATa                                                                                                                              <  1:359941/105‑1 (MQ=255)
                                     ttCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATa                                                                                                                              >  2:359941/1‑105 (MQ=255)
                                               tttCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTcgcg                                                                                  <  1:77396/139‑1 (MQ=255)
                                                    aGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTcg                                                                                    >  1:355262/1‑132 (MQ=255)
                                                    aGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTcg                                                                                    <  2:355262/132‑1 (MQ=255)
                                                    aGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGtt                                                                             <  1:345939/139‑1 (MQ=255)
                                                           aCGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAAt                                                                                                                >  1:421678/1‑97 (MQ=255)
                                                           aCGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAAt                                                                                                                <  2:421678/97‑1 (MQ=255)
                                                                              ctcGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCg                                                   >  2:168404/1‑139 (MQ=255)
                                                                              ctcGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCg                                                   >  2:284476/1‑139 (MQ=255)
                                                                                cGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGAc                                                 <  2:338806/139‑1 (MQ=255)
                                                                                        agaTCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTaaa                                                                                                       >  1:500294/1‑77 (MQ=255)
                                                                                        agaTCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTaaa                                                                                                       <  2:500294/77‑1 (MQ=255)
                                                                                              aCCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGAGCGGTGACGATAATATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGTGGCGTTCAGAGGTCAATTTTACTTCACAAGCGACCACATCATTACGcc                                   <  1:617647/139‑1 (MQ=255)
                                                                                                  cACGCGCAACCGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCttt                               >  1:228154/1‑139 (MQ=255)
                                                                                                           ccGCTGCGTCTTTCAGGTCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGAt                      <  2:200701/139‑1 (MQ=255)
                                                                                                                    cTTTCAGGTCGCGATCGGTGACAATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACcac             <  1:585836/139‑1 (MQ=255)
                                                                                                                            tCGCGATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATgg     <  1:26140/139‑1 (MQ=255)
                                                                                                                              gcgATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCa   <  2:228154/139‑1 (MQ=255)
                                                                                                                               cgATCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCAc  <  2:304270/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
AAGTCACGCGGCAACCACTTCATACGAGTGAGTAATATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCAC  >  NC_000913/3485411‑3485676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑