Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,607,418 C→T A323V (GCC→GTC)  zntA → zinc, cobalt and lead efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,607,4180CT100.0% 96.5 / NA 29A323V (GCC→GTC) zntAzinc, cobalt and lead efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/13);  total (16/13)

TTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGTGACG  >  NC_000913/3607286‑3607548
                                                                                                                                    |                                                                                                                                  
ttGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGCCGTTGGAAGTGCTGTCAGAACCGGGAGTCAgcgc                                                                                                                              >  1:362523/1‑139 (MQ=255)
         ttGATGAAAGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCg                                                                                                                     <  1:178145/139‑1 (MQ=255)
                 aGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGa                                                                                                             >  2:364428/1‑139 (MQ=255)
                   cgcCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGaaa                                                                                                           <  1:350261/139‑1 (MQ=255)
                         gACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATc                                                                                                     >  2:493755/1‑139 (MQ=255)
                                             tGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCAt                                                                                                                           <  1:94173/97‑1 (MQ=255)
                                             tGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCAt                                                                                                                           >  2:94173/1‑97 (MQ=255)
                                                  cgcgcAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTc                                                                            >  2:571403/1‑139 (MQ=255)
                                                      cAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg                                                                               <  1:277936/132‑1 (MQ=255)
                                                      cAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg                                                                               >  2:277936/1‑132 (MQ=255)
                                                      cAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCAt                                                                        >  1:281376/1‑139 (MQ=255)
                                                           ggCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGc                                                                   <  2:20564/139‑1 (MQ=255)
                                                              gATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGt                                                                <  1:571403/139‑1 (MQ=255)
                                                                                          tAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATAc                                    >  1:571970/1‑139 (MQ=255)
                                                                                                 tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGa                                                         <  1:468877/111‑1 (MQ=255)
                                                                                                 tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGa                                                         >  2:468877/1‑111 (MQ=255)
                                                                                                 tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAg                                                >  2:489608/1‑120 (MQ=255)
                                                                                                 tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAg                                                <  1:489608/120‑1 (MQ=255)
                                                                                                 tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGccc                                >  2:435335/1‑136 (MQ=255)
                                                                                                 tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCcgcg                             >  2:539863/1‑139 (MQ=255)
                                                                                                 tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAACAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGccc                                <  1:435335/136‑1 (MQ=255)
                                                                                                  cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCgaag                                                                                                 >  1:424608/1‑70 (MQ=255)
                                                                                                  cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCgaag                                                                                                 <  2:424608/70‑1 (MQ=255)
                                                                                                         cGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGt                                            >  1:523735/1‑116 (MQ=255)
                                                                                                         cGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGt                                            <  2:523735/116‑1 (MQ=255)
                                                                                                                        cAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGt      >  2:283397/1‑139 (MQ=255)
                                                                                                                            aCCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGTGACg  <  1:364428/139‑1 (MQ=255)
                                                                                                                                 gAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGa                                                         >  1:557761/1‑79 (MQ=255)
                                                                                                                                 gAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGa                                                         <  2:557761/79‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                  
TTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGTGACG  >  NC_000913/3607286‑3607548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: