Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,004	A→G	G114G (GGT→GGC)	tdh ←	L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,004	0	A	G	100.0%	62.2 / NA	18	G114G (GGT→GGC)	tdh	L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

ACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCT  >  NC_000913/3790868‑3791125                                                                                                                                         |                                                                                                                          aCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGcc                                                                                                                         >  2:92901/1‑139 (MQ=255)                gCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGtt                                                                                                          <  2:344273/139‑1 (MQ=255)                      cGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGca                                                                                                    >  1:596576/1‑139 (MQ=255)                      cGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGca                                                                                                    >  2:594675/1‑139 (MQ=255)                         tACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaa                                                                                                 >  2:41073/1‑139 (MQ=255)                                                            gAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAc                                                              <  2:568623/139‑1 (MQ=255)                                                                tatTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAAt                                                                 <  2:614401/132‑1 (MQ=255)                                                                tatTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAAt                                                                 >  1:614401/1‑132 (MQ=255)                                                                  ttGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAgg                                                        <  1:594675/139‑1 (MQ=255)                                                                       ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa                                                                   >  2:370345/1‑123 (MQ=255)                                                                       ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa                                                                   <  1:370345/123‑1 (MQ=255)                                                                       ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGata                                                   <  1:41073/139‑1 (MQ=255)                                                                                                 ggATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGcc                         <  2:596576/139‑1 (MQ=255)                                                                                                               tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGGGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGccgt           >  1:596555/1‑137 (MQ=255)                                                                                                                cGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCttt          <  1:92901/139‑1 (MQ=255)                                                                                                                 ggCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                         <  1:633666/91‑1 (MQ=255)                                                                                                                 ggCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                         >  2:633666/1‑91 (MQ=255)                                                                                                                        cagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCt  <  2:484254/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                          ACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCT  >  NC_000913/3790868‑3791125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑