Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,901,992 C→T A77T (GCC→ACC)  bglH ← carbohydrate‑specific outer membrane porin, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,901,9920CT100.0% 80.6 / NA 24A77T (GCC→ACC) bglHcarbohydrate‑specific outer membrane porin, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/11);  total (13/11)

ATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTAAC  >  NC_000913/3901858‑3902101
                                                                                                                                      |                                                                                                             
aTCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTGCTGGTCGGGAACGGATTGGTGGCa                                                                                                           <  1:370057/139‑1 (MQ=255)
aTCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCa                                                                                                           <  2:533297/139‑1 (MQ=255)
        caAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTg                                                                                                   <  2:429632/139‑1 (MQ=255)
                    aaTCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACt                                                                                       <  2:97079/139‑1 (MQ=255)
                      tCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTa                                                                                     <  1:567252/139‑1 (MQ=255)
                          tCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCt                                                                                 >  1:348925/1‑139 (MQ=255)
                                tCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGAtt                                                                           >  2:40799/1‑139 (MQ=255)
                                          aTAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCt                                                                 >  2:311765/1‑139 (MQ=255)
                                              aTCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCg                                                             <  2:205501/139‑1 (MQ=255)
                                                         ctcACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTttc                                                  <  2:348925/139‑1 (MQ=255)
                                                                                     aCCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGtt                      >  1:341388/1‑139 (MQ=255)
                                                                                          tACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCtt                 >  1:79829/1‑139 (MQ=255)
                                                                                               cATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGt            >  1:578047/1‑139 (MQ=255)
                                                                                                 tCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGttt          <  2:612855/139‑1 (MQ=255)
                                                                                                    ggTTTTGCGGCACTACTGGTCGGGAACGGATTggtggc                                                                                                            <  1:20741/38‑1 (MQ=255)
                                                                                                    ggTTTTGCGGCACTACTGGTCGGGAACGGATTggtggc                                                                                                            >  2:20741/1‑38 (MQ=255)
                                                                                                    ggTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTtctc       >  1:263101/1‑139 (MQ=255)
                                                                                                    ggTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTtctc       >  1:430047/1‑139 (MQ=255)
                                                                                                    ggTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTtctc       >  1:551367/1‑139 (MQ=255)
                                                                                                    ggTTTTGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTtctc       >  2:558095/1‑139 (MQ=255)
                                                                                                         tGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTAAc  <  2:341388/139‑1 (MQ=255)
                                                                                                         tGCGGCACTACTGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTAAc  >  1:396074/1‑139 (MQ=255)
                                                                                                                   ctGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCg                                                                                           <  2:523180/40‑1 (MQ=255)
                                                                                                                   ctGGTCGGGAACGGATTGGTGGCATACCCTTGATCATTCg                                                                                           >  1:523180/1‑40 (MQ=255)
                                                                                                                                gATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCg                                                                   >  1:31596/1‑51 (MQ=255)
                                                                                                                                gATTGGTGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCg                                                                   <  2:31596/51‑1 (MQ=255)
                                                                                                                                      |                                                                                                             
ATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTAAC  >  NC_000913/3901858‑3902101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: